BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	96,614	A→G	T205A (ACC→GCC)	mraY →	phospho‑N‑acetylmuramoyl‑pentapeptide transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	96,614	0	A	G	100.0%	427.7 / NA	115	T205A (ACC→GCC)	mraY	phospho‑N‑acetylmuramoyl‑pentapeptide transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (47/68); total (47/68)

Only 100 of 117 total aligned reads displayed.

GCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCC  >  NC_000913/96466‑96761

   cGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGt                                                                                                                                                 >  2:277549/1‑150 (MQ=255)

      aaCGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTAttt                                                                                                                                             <  3:836253/151‑1 (MQ=255)

       aCGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTg                                                                                                                                            >  2:1252161/1‑151 (MQ=255)

        cGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGt                                                                                                                                           <  2:1190232/151‑1 (MQ=255)

         gCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTc                                                                                                                                          >  4:229716/1‑151 (MQ=255)

          cAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCg                                                                                                                                         <  3:1202894/151‑1 (MQ=255)

           aGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCg                                                                                                                                         >  1:92284/1‑150 (MQ=255)

             cTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCg                                                                                                                                      >  2:565665/1‑151 (MQ=255)

               ggtggtCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCgg                                                                                                                                     <  2:1293162/150‑1 (MQ=255)

               ggtggtCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCg                                                                                                                                      <  2:470285/149‑1 (MQ=255)

                gtggtCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCggtg                                                                                                                                   <  3:543261/151‑1 (MQ=255)

                  ggtCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCggtgg                                                                                                                                  <  4:187067/150‑1 (MQ=255)

                     cccATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGtttt                                                                                                                              <  4:424393/151‑1 (MQ=255)

                     cccATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGtttt                                                                                                                              <  3:313349/151‑1 (MQ=255)

                     cccATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGttt                                                                                                                               <  3:141500/150‑1 (MQ=255)

                       cATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTgc                                                                                                                            <  2:528265/151‑1 (MQ=255)

                       cATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTgc                                                                                                                            <  2:386513/151‑1 (MQ=255)

                       cATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTgc                                                                                                                            <  1:163031/151‑1 (MQ=255)

                        aTTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTgcg                                                                                                                           >  2:1314822/1‑151 (MQ=255)

                        aTTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTgcg                                                                                                                           >  2:574552/1‑151 (MQ=255)

                         ttCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTgcg                                                                                                                           >  2:818155/1‑150 (MQ=255)

                             ttAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCtg                                                                                                                        >  4:371710/1‑149 (MQ=255)

                             ttAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCtg                                                                                                                        <  3:14322/149‑1 (MQ=255)

                              tAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCtggtg                                                                                                                     >  2:1232027/1‑151 (MQ=255)

                               aaaGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCtggtg                                                                                                                     <  2:149987/150‑1 (MQ=255)

                                aaGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGc                                                                                                                   <  1:51432/151‑1 (MQ=255)

                                    tgtgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTg                                                                                                                <  1:102675/150‑1 (MQ=255)

                                     gtgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTgg                                                                                                               <  3:452256/150‑1 (MQ=255)

                                     gtgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTgg                                                                                                               <  2:643088/150‑1 (MQ=255)

                                        aTGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCg                                                                                                            >  1:114791/1‑150 (MQ=255)

                                        aTGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGa                                                                                                           <  2:660776/151‑1 (MQ=255)

                                            cGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCgg                                                                                                       <  2:39531/151‑1 (MQ=255)

                                            cGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACGTCGTCAATGTGGGTACTGGCAACGCGGTAAACCTGACCGTTGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGTTTTTGCGCTGGTGGCGTGGGCGAc                                                                                                          <  4:438762/148‑1 (MQ=255)

                                               aGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCaa                                                                                                    <  3:954906/151‑1 (MQ=255)

                                                gCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAat                                                                                                   <  3:693940/151‑1 (MQ=255)

                                                   ggggCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATg                                                                                                <  3:524539/151‑1 (MQ=255)

                                                    gggCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGa                                                                                               <  1:412019/151‑1 (MQ=255)

                                                     ggCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGaa                                                                                              <  2:1390499/151‑1 (MQ=255)

                                                         gTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACt                                                                                            <  3:1051771/149‑1 (MQ=255)

                                                           tCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTg                                                                                         <  3:11896/150‑1 (MQ=255)

                                                             tACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGcc                                                                                       >  4:195718/1‑150 (MQ=255)

                                                              aCATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAg                                                                                     <  3:1281675/151‑1 (MQ=255)

                                                                aTTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCt                                                                                   <  4:426696/151‑1 (MQ=255)

                                                                   ctgctgGCTTACTTCATCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACt                                                                                >  2:1147339/1‑151 (MQ=255)

                                                                     gctgGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACtt                                                                               >  3:1282023/1‑150 (MQ=255)

                                                                     gctgGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTg                                                                              >  1:111080/1‑151 (MQ=255)

                                                                     gctgGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTg                                                                              >  2:344615/1‑151 (MQ=255)

                                                                        ggCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGc                                                                             <  3:1314892/149‑1 (MQ=255)

                                                                        ggCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCat                                                                           <  3:384325/151‑1 (MQ=255)

                                                                        ggCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCa                                                                            <  3:565696/150‑1 (MQ=255)

                                                                        ggCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCa                                                                            <  3:1343440/150‑1 (MQ=255)

                                                                         gCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCata                                                                          <  1:198482/151‑1 (MQ=255)

                                                                         gCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCata                                                                          <  1:69784/151‑1 (MQ=255)

                                                                          cTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCatat                                                                         >  2:828989/1‑151 (MQ=255)

                                                                            tACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGATCTTTGCCAGCTACTTGCataga                                                                        <  4:480210/150‑3 (MQ=255)

                                                                            tACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCAtata                                                                        <  3:1228477/150‑1 (MQ=255)

                                                                              cTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCg                                                                     >  2:1232268/1‑151 (MQ=255)

                                                                                tCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGt                                                                    >  2:819138/1‑150 (MQ=255)

                                                                                tCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGt                                                                    >  2:520454/1‑150 (MQ=255)

                                                                                tCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGt                                                                    >  2:194013/1‑150 (MQ=255)

                                                                                 cGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTa                                                                   <  3:1157607/150‑1 (MQ=255)

                                                                                  gTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATc                                                                 >  2:1326233/1‑151 (MQ=255)

                                                                                  gTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATc                                                                 >  2:1252739/1‑151 (MQ=255)

                                                                                   tCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCt                                                                >  3:96228/1‑151 (MQ=255)

                                                                                       tgtgGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGca                                                             >  2:89031/1‑149 (MQ=255)

                                                                                       tgtgGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCg                                                             >  2:363599/1‑150 (MQ=255)

                                                                                              aCTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGcc                                                      <  2:5019/150‑1 (MQ=255)

                                                                                                 ggCAACGCGGTAAACCTGACCGATGTTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCgggg                                                  <  3:818189/151‑1 (MQ=255)

                                                                                                 ggCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCgggg                                                  >  2:1270127/1‑151 (MQ=255)

                                                                                                 ggCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCggg                                                   <  3:277576/150‑1 (MQ=255)

                                                                                                 ggCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCggg                                                   <  3:385365/150‑1 (MQ=255)

                                                                                                   cAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGaa                                                <  2:1239835/151‑1 (MQ=255)

                                                                                                     aCGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACt                                              >  3:961836/1‑151 (MQ=255)

                                                                                                     aCGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACt                                              >  3:795776/1‑151 (MQ=255)

                                                                                                     aCGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACt                                              >  2:137862/1‑151 (MQ=255)

                                                                                                      cgcgGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTg                                             >  3:809656/1‑151 (MQ=255)

                                                                                                          gTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGtt                                          >  2:207527/1‑150 (MQ=255)

                                                                                                                 tGACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCt                                   >  3:1330117/1‑150 (MQ=255)

                                                                                                                  gACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCt                                   <  2:1023349/149‑1 (MQ=255)

                                                                                                                  gACCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGt                                 >  3:959879/1‑151 (MQ=255)

                                                                                                                   aCCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGt                                 >  4:280506/1‑150 (MQ=255)

                                                                                                                   aCCGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTa                                <  1:229718/151‑1 (MQ=255)

                                                                                                                     cGATGGTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTAcc                              <  3:940942/151‑1 (MQ=255)

                                                                                                                         ggTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACcgcg                           >  4:220847/1‑150 (MQ=255)

                                                                                                                          gTCTCGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGAt                         <  3:868355/151‑1 (MQ=255)

                                                                                                                              cGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATa                        <  4:182053/148‑1 (MQ=255)

                                                                                                                              cGACGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGt                      <  2:1298054/150‑1 (MQ=255)

                                                                                                                                 cGGCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCggg                  <  3:137879/151‑1 (MQ=255)

                                                                                                                                  ggCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCgggg                 >  3:98416/1‑151 (MQ=255)

                                                                                                                                   gCCTGGCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGc                <  2:96214/151‑1 (MQ=255)

                                                                                                                                        gCAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGAc           >  3:284009/1‑151 (MQ=255)

                                                                                                                                         cAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACt          <  3:1232086/151‑1 (MQ=255)

                                                                                                                                         cAATTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACt          <  3:1252223/151‑1 (MQ=255)

                                                                                                                                          aaTTATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTg         >  3:1025735/1‑151 (MQ=255)

                                                                                                                                             tATGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGc      >  1:355751/1‑151 (MQ=255)

                                                                                                                                              aTGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTGCTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGc      <  2:531854/150‑1 (MQ=255)

                                                                                                                                              aTGCCGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCt     >  2:1200821/1‑151 (MQ=255)

                                                                                                                                                gccggccgTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTc   <  3:683591/151‑1 (MQ=255)

                                                                                                                                                 ccGGCCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTcc  >  3:845324/1‑151 (MQ=255)

                                                                                                                                                 ccGGCCGTATTTGTCGCCGGCGGTTTTGCGCTGGTCGCGCGGGCGACCGGCAATATGAACTTTTCCAGCTACTCGCATATACTGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGCCGGGGCCGGACTggg       >  3:751527/1‑146 (MQ=255)

GCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACCGTATCTGCGACACGCCGGGGAACTGGTTATTGTCTGTACCGCGATAGTCGGGGCAGGACTGGGCTTCC  >  NC_000913/96466‑96761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`