Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 657,377 T→C V29A (GTG→GCG)  cspE → constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913657,3770TC100.0% 533.3 / NA 144V29A (GTG→GCG) cspEconstitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (72/72);  total (72/72)

Only 100 of 144 total aligned reads displayed.

CAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAA  >  NC_000913/657230‑657522
                                                                                                                                                   |                                                                                                                                                 
caATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGtt                                                                                                                                                <  3:490070/151‑1 (MQ=255)
 aaTTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTc                                                                                                                                               <  3:672955/151‑1 (MQ=255)
 aaTTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTc                                                                                                                                               <  3:1172594/151‑1 (MQ=255)
    tGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGt                                                                                                                                             <  3:634565/150‑1 (MQ=255)
     ggTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTac                                                                                                                                           >  3:241127/1‑151 (MQ=255)
     ggTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTa                                                                                                                                            <  2:278215/150‑1 (MQ=255)
     ggTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTa                                                                                                                                            >  1:49231/1‑150 (MQ=255)
        gtACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACt                                                                                                                                        >  2:859405/1‑151 (MQ=255)
          aCTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACtt                                                                                                                                       <  3:1215780/150‑1 (MQ=255)
          aCTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTtc                                                                                                                                      <  2:760350/151‑1 (MQ=255)
           cTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTtct                                                                                                                                     >  2:448335/1‑151 (MQ=255)
             ggTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTtct                                                                                                                                     <  2:631508/149‑1 (MQ=255)
               tAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGc                                                                                                                                 >  1:389958/1‑151 (MQ=255)
               tAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGc                                                                                                                                 >  2:731069/1‑151 (MQ=255)
                  ccGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAAt                                                                                                                              >  3:674543/1‑151 (MQ=255)
                   cGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATc                                                                                                                             <  4:166861/151‑1 (MQ=255)
                    gACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATcc                                                                                                                            >  3:273536/1‑151 (MQ=255)
                       acaGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATcc                                                                                                                            >  2:622088/1‑148 (MQ=255)
                         aGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACt                                                                                                                       >  2:748707/1‑151 (MQ=255)
                         aGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACt                                                                                                                       <  3:988648/151‑1 (MQ=255)
                            aTTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAAt                                                                                                                    <  3:627256/151‑1 (MQ=255)
                              ttGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATg                                                                                                                   <  3:1394719/150‑1 (MQ=255)
                                   tCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGtttt                                                                                                              >  2:173308/1‑150 (MQ=255)
                                   tCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGtttt                                                                                                              >  2:466785/1‑150 (MQ=255)
                                     tATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTaa                                                                                                            >  2:61195/1‑150 (MQ=255)
                                      aTTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTaaa                                                                                                           >  4:2688/1‑150 (MQ=255)
                                      aTTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTaaa                                                                                                           <  4:380755/150‑1 (MQ=255)
                                      aTTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTaaa                                                                                                           <  3:72439/150‑1 (MQ=255)
                                      aTTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTa                                                                                                             <  2:93834/148‑1 (MQ=255)
                                        ttttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAAct                                                                                                        >  1:321002/1‑151 (MQ=255)
                                         tttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAActc                                                                                                       >  3:1371100/1‑151 (MQ=255)
                                            cATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTg                                                                                                    <  3:571700/151‑1 (MQ=255)
                                               gTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGca                                                                                                  <  3:718945/150‑2 (MQ=255)
                                                tAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTg                                                                                                 >  2:667116/1‑150 (MQ=255)
                                                 aaaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGa                                                                                                <  2:241102/150‑1 (MQ=255)
                                                 aaaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGa                                                                                                >  1:86849/1‑150 (MQ=255)
                                                  aaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGa                                                                                                >  1:321060/1‑149 (MQ=255)
                                                    ggTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGt                                                                                            <  2:73638/151‑1 (MQ=255)
                                                     gTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTc                                                                                           <  4:472647/151‑1 (MQ=255)
                                                      tAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGt                                                                                            >  3:1338251/1‑149 (MQ=255)
                                                      tAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTc                                                                                           >  2:1081368/1‑150 (MQ=255)
                                                       aaTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTc                                                                                           >  3:134870/1‑149 (MQ=255)
                                                       aaTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTc                                                                                           >  3:1076592/1‑149 (MQ=255)
                                                       aaTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCa                                                                                          <  3:2851/150‑1 (MQ=255)
                                                        aTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAgc                                                                                        <  3:473716/151‑1 (MQ=255)
                                                          tttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACGCTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAgcgc                                                                                      >  3:1238606/1‑151 (MQ=255)
                                                          tttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAgcgc                                                                                      >  3:1007399/1‑151 (MQ=255)
                                                          tttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAgcg                                                                                       <  2:843681/150‑1 (MQ=255)
                                                          tttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAgcg                                                                                       <  4:123523/150‑1 (MQ=255)
                                                             gATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGt                                                                                    >  3:1244402/1‑150 (MQ=255)
                                                             gATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGt                                                                                    >  2:1044507/1‑150 (MQ=255)
                                                                gTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTaga                                                                                 <  4:151564/150‑1 (MQ=255)
                                                                      gATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGa                                                                           >  2:654873/1‑150 (MQ=255)
                                                                       aTTAAAGGAAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGaa                                                                          >  2:692495/1‑150 (MQ=255)
                                                                         tAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATc                                                                       >  2:1090473/1‑151 (MQ=255)
                                                                          aaaGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATc                                                                       <  2:1202482/150‑1 (MQ=255)
                                                                           aaGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCAc                                                                     >  2:442449/1‑151 (MQ=255)
                                                                            aGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACt                                                                    >  1:245953/1‑151 (MQ=255)
                                                                            aGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACt                                                                    >  2:793114/1‑151 (MQ=255)
                                                                                aaCGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAAc                                                                 <  3:1358964/150‑1 (MQ=255)
                                                                                aaCGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACg                                                                <  2:109983/151‑1 (MQ=255)
                                                                                      aaGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGcc                                                           >  3:967077/1‑150 (MQ=255)
                                                                                        gTGGTTTAATGAGACCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCaaa                                                        <  3:775571/151‑1 (MQ=255)
                                                                                          ggTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAgg                                                      <  3:448369/151‑1 (MQ=255)
                                                                                             ttAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGcc                                                    <  3:145305/150‑1 (MQ=255)
                                                                                              tAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCt                                                  >  1:37076/1‑151 (MQ=255)
                                                                                              tAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCt                                                  >  2:969156/1‑151 (MQ=255)
                                                                                                  gAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctg                                              <  3:523027/151‑1 (MQ=255)
                                                                                                  gAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctg                                              <  2:134853/151‑1 (MQ=255)
                                                                                                  gAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTca                                               <  3:800836/150‑2 (MQ=255)
                                                                                                   aGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctgc                                             >  2:74320/1‑151 (MQ=255)
                                                                                                        aaaGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCaa                                        <  3:1176593/151‑1 (MQ=255)
                                                                                                        aaaGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCa                                         >  3:706906/1‑150 (MQ=255)
                                                                                                           ggATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACg                                     >  2:779262/1‑151 (MQ=255)
                                                                                                             aTTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGt                                    >  3:850710/1‑150 (MQ=255)
                                                                                                               tCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTaa                                  <  3:1039320/150‑1 (MQ=255)
                                                                                                               tCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAAt                                 >  1:379639/1‑151 (MQ=255)
                                                                                                                 ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGTAAACGTAATCg                               >  3:560013/1‑151 (MQ=255)
                                                                                                                 ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                               <  4:76293/151‑1 (MQ=255)
                                                                                                                 ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                               >  3:22105/1‑151 (MQ=255)
                                                                                                                 ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                               <  2:273509/151‑1 (MQ=255)
                                                                                                                  gTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGc                              >  2:1223559/1‑151 (MQ=255)
                                                                                                                    ttCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGct                             <  4:35602/150‑1 (MQ=255)
                                                                                                                    ttCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGct                             <  2:654590/150‑1 (MQ=255)
                                                                                                                      cATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTg                          <  1:108007/151‑1 (MQ=255)
                                                                                                                      cATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTg                          >  2:311565/1‑151 (MQ=255)
                                                                                                                         tACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTa                        >  1:48317/1‑150 (MQ=255)
                                                                                                                             ccGGAAGACGGCAGAAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGAGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGa                     <  2:168711/149‑1 (MQ=255)
                                                                                                                                 aaGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTc               >  3:377785/1‑151 (MQ=255)
                                                                                                                                   gACGGCAGCAAAGACGCGTTCGTACGCTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAg             <  2:1238547/151‑1 (MQ=255)
                                                                                                                                   gACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTc               >  2:1139270/1‑149 (MQ=255)
                                                                                                                                   gACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAg             <  3:125247/151‑1 (MQ=255)
                                                                                                                                   gACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAg             <  3:567033/151‑1 (MQ=255)
                                                                                                                                     cGGCAGCAAAGACGCGTTCGTACACTACTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAAGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCa           <  3:849555/151‑1 (MQ=255)
                                                                                                                                       gcagcaAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAg         <  1:401309/151‑1 (MQ=255)
                                                                                                                                        cagcaAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGa        <  2:1371031/151‑1 (MQ=255)
                                                                                                                                          gcaAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAt      <  1:144493/151‑1 (MQ=255)
                                                                                                                                           caAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAtt     <  3:1220743/151‑1 (MQ=255)
                                                                                                                                             aaGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTc    >  1:361819/1‑150 (MQ=255)
                                                                                                                                              aGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCa   >  4:189327/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAA  >  NC_000913/657230‑657522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑