BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	704,128	C→T	S62F (TCC→TTC)	nagE →	N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	704,128	0	C	T	100.0%	440.3 / NA	110	S62F (TCC→TTC)	nagE	N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (54/56); total (54/56)

Only 100 of 110 total aligned reads displayed.

CGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCATTACCGGTCTGGTTGGTGGCGCAG  >  NC_000913/703981‑704274

 gTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGc                                                                                                                                                       >  2:1017148/1‑151 (MQ=255)

  ttACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCt                                                                                                                                                      >  3:23895/1‑151 (MQ=255)

   tACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCt                                                                                                                                                      <  2:41946/150‑1 (MQ=255)

    aCAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTg                                                                                                                                                     <  2:1221737/150‑1 (MQ=255)

              aTCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACa                                                                                                                                          <  2:1000116/151‑1 (MQ=255)

                cgcgGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAgc                                                                                                                                        >  3:924807/1‑151 (MQ=255)

                   ggTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCt                                                                                                                                     <  2:1398191/151‑1 (MQ=255)

                   ggTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCt                                                                                                                                     <  2:1248287/151‑1 (MQ=255)

                   ggTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCt                                                                                                                                     <  2:643344/151‑1 (MQ=255)

                      gctgcCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTgg                                                                                                                                   <  3:518219/150‑1 (MQ=255)

                         gcCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTgcg                                                                                                                               >  2:1311744/1‑151 (MQ=255)

                         gcCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTgc                                                                                                                                <  4:191967/150‑1 (MQ=255)

                         gcCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTg                                                                                                                                 <  2:1396279/149‑1 (MQ=255)

                         gcCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTg                                                                                                                                 <  4:215881/149‑1 (MQ=255)

                          ccGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTgcgg                                                                                                                              >  3:1143864/1‑151 (MQ=255)

                          ccGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTgcgg                                                                                                                              >  1:119077/1‑151 (MQ=255)

                           cGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTgcggc                                                                                                                             <  4:347025/151‑1 (MQ=255)

                            ggTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTgcggcg                                                                                                                            >  1:75419/1‑151 (MQ=255)

                             gTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTgcggcgg                                                                                                                           <  2:662323/151‑1 (MQ=255)

                               ggcggcACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTgcggcggc                                                                                                                          <  2:274058/150‑1 (MQ=255)

                                 cggcACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCt                                                                                                                       <  2:42027/151‑1 (MQ=255)

                                  ggcACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTg                                                                                                                      <  3:508558/151‑1 (MQ=255)

                                   gcACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTgg                                                                                                                     >  2:194049/1‑151 (MQ=255)

                                   gcACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTgg                                                                                                                     <  2:185102/151‑1 (MQ=255)

                                    cACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTgg                                                                                                                     >  3:908439/1‑150 (MQ=255)

                                    cACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTgg                                                                                                                     >  1:211203/1‑150 (MQ=255)

                                    cACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGc                                                                                                                    <  3:138495/151‑1 (MQ=255)

                                          tgctgcGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCgggggg                                                                                                              >  3:155319/1‑148 (MQ=255)

                                           gctgcGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCggggg                                                                                                               >  2:674218/1‑147 (MQ=255)

                                           gctgcGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGc                                                                                                              <  2:875963/150‑1 (MQ=255)

                                           gctgcGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑g                                                                                                            <  2:84607/151‑1 (MQ=255)

                                              gcGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCgggggggg                                                                                                            >  2:728904/1‑144 (MQ=255)

                                                gATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑gg                                                                                                      <  1:22881/151‑1 (MQ=255)

                                                  ttCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCgggggggggggggt                                                                                                      >  2:49277/1‑140 (MQ=255)

                                                        cAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑ttt                                                                                              <  4:46952/150‑1 (MQ=255)

                                                        cAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGGG‑GGTTTC‑tttt                                                                                             >  3:323938/1‑150 (MQ=255)

                                                          gCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGC‑gggggggttcttttt                                                                                              >  3:453590/1‑137 (MQ=255)

                                                             aGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTa                                                                                       <  4:291900/151‑1 (MQ=255)

                                                              gATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTaa                                                                                      <  3:632077/151‑1 (MQ=255)

                                                               aTTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAAc                                                                                     <  2:1297972/151‑1 (MQ=255)

                                                               aTTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGC‑GGGA‑GGGTAC‑TTTGTG‑ttta                                                                                      >  2:718029/1‑148 (MQ=255)

                                                                 ttACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGTG‑GGGTAC‑ATTTTG‑tt                                                                                        >  3:313754/1‑146 (MQ=255)

                                                                  tACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGG‑GGGA‑GGGTAC‑TTTGTG‑TTAAcc                                                                                    >  2:420409/1‑149 (MQ=255)

                                                                  tACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGGG‑GGGTTC‑TTTTTG‑TTaaac                                                                                    >  1:359005/1‑147 (MQ=255)

                                                                   aCTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCaaa                                                                                 <  4:395701/151‑1 (MQ=255)

                                                                    cTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGC‑GGGG‑GGGTTC‑CTTTTG‑TTAACCaaa                                                                                 >  2:1140609/1‑150 (MQ=255)

                                                                     ttAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGTA‑GGGTAC‑TTTGTG‑TTaaaccaa                                                                                 >  2:711582/1‑144 (MQ=255)

                                                                      tAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAg                                                                                <  4:220854/149‑1 (MQ=255)

                                                                      tAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAg                                                                                <  3:1403898/149‑1 (MQ=255)

                                                                       aaCGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGa                                                                             <  4:139405/151‑1 (MQ=255)

                                                                       aaCGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGGG‑GGGTTC‑TTTTTG‑TTTAACaaaa                                                                                >  3:1176590/1‑147 (MQ=255)

                                                                       aaCGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGACGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCg                                                                              >  1:27366/1‑150 (MQ=255)

                                                                        aCGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGG‑GGGG‑GGTTAT‑TTTTTT‑TTTAACaaa                                                                                 >  3:135241/1‑146 (MQ=255)

                                                                                tttATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGGAGGGTTAT‑TTTTGT‑TTAACCaaaaggagg                                                                           >  2:1154074/1‑139 (MQ=255)

                                                                                  tATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCAt                                                                   <  2:112401/150‑1 (MQ=255)

                                                                                  tATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGGA‑GGGTGA‑TTTTTT‑GTAACCAAAACGGTgggggacccc                                                                  >  3:69632/1‑143 (MQ=255)

                                                                                  tATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGGA‑GGGTAC‑TTTGTG‑TTAAACAAAACGATGGGGACCa                                                                    >  1:423643/1‑149 (MQ=255)

                                                                                   aTTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑a                                                                <  4:43011/151‑1 (MQ=255)

                                                                                    ttGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGGG‑GGGTTC‑TTTTTGTTTAACCAAA‑AGATGGTGGcc                                                                     >  3:862199/1‑146 (MQ=255)

                                                                                     tGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGGA‑GGGTAA‑TTTTTG‑TTAAACAAAAGGATGGGGGCCATC‑aa                                                               >  2:2553/1‑150 (MQ=255)

                                                                                        ccAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCa                                                          <  2:508514/151‑1 (MQ=255)

                                                                                         cAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAg                                                         <  1:174885/151‑1 (MQ=255)

                                                                                             cgggcggTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAAt                                                    <  2:1089102/151‑1 (MQ=255)

                                                                                              gggcggTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGG‑GGGT‑GGGTAC‑TTTGTG‑TTAACCAAAACGATGGGGACCATC‑AA‑CCCAG‑aaaat                                                   >  1:383388/1‑149 (MQ=255)

                                                                                              gggcggTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAAtt                                                   <  2:178873/151‑1 (MQ=255)

                                                                                                   gTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACAt                                              <  3:1311814/151‑1 (MQ=255)

                                                                                                      cGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGGGG‑GGTTAC‑TTT‑TG‑TTTAACAAAACGGTGGTGACCCTC‑CA‑CCCAG‑AAATTAACATggg                                           >  4:257354/1‑151 (MQ=255)

                                                                                                       gATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGgt                                          <  2:323908/151‑1 (MQ=255)

                                                                                                        aTTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGgtg                                         <  3:82061/151‑1 (MQ=255)

                                                                                                        aTTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGgtg                                         <  3:409617/151‑1 (MQ=255)

                                                                                                            ttGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGC‑GGGA‑GGGTTA‑TTTTTG‑TTTAACAAAAAGATGGGGACCCTCAAA‑CCCAC‑AAATTAAAAggggggaat                                      >  3:1204790/1‑146 (MQ=255)

                                                                                                            ttGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGC‑GGGA‑GGGTAC‑TTTTTG‑GTTAACAAAACGGAGGGGACCATC‑CA‑ACCCG‑AAATTAAAATggggg                                         >  3:561662/1‑145 (MQ=255)

                                                                                                             tGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGC‑GGGA‑GGTTAC‑TTTGTGTTTACCCAAAAGCAGGGGGACC‑TC‑AA‑CCCCGAAAATTACCATgggg                                          >  3:659734/1‑145 (MQ=255)

                                                                                                                 aaCCTCGCATTAATCTTCGCCATCGGTGTGGCCTTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGTC‑GGGTGC‑TTTTTGTTTAACCAAAG‑GACAGTGTGCCTC‑CA‑ACCCG‑AAATTAACaaaaggggaccatcgg                                >  3:1226579/1‑136 (MQ=255)

                                                                                                                   ccTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGGTAC‑TTTGTG‑TTTACCAAAACGATGGTGACCATC‑AA‑CCCAG‑AAATTAAAATGGGTGTACTGGCgggg                              >  1:323908/1‑150 (MQ=255)

                                                                                                                    cTCGCATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGGA‑GGGTTC‑TTTTTGTTTAACCAAA‑CGGAGGTGACCCTC‑AA‑CCCAG‑AAAATAACATGGGGGGACTGGCgggg                              >  2:531668/1‑149 (MQ=255)

                                                                                                                        cATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGGA‑GGGTACTTTTTTT‑TTAACAAAAGGGAGGGGGACAACA‑AA‑CCCAA‑AAATTAAAATGGG‑GGACCGGCggggg                             >  3:52643/1‑145 (MQ=255)

                                                                                                                        cATTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGC‑GGGA‑GGGTAC‑TTTGTG‑TTAACCAAAGCGATGGGGGACAAC‑AA‑ACCAG‑AAAATAACATGGGGGTAATGGCggggttta                          >  1:370253/1‑145 (MQ=255)

                                                                                                                         aTTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCAtt                        <  3:551793/151‑1 (MQ=255)

                                                                                                                         aTTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGGTAC‑TTTTTG‑GTAACCAAAACGGGGGGGGGCATC‑AA‑CCCAG‑AAAAAAACATGGGTGTAATgggggg                               >  3:397298/1‑144 (MQ=255)

                                                                                                                         aTTAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGC‑GGGG‑GGTTAC‑TTTTTG‑TTTACCAAAACGGTGGTGGCCCTC‑CA‑CCCAC‑AAAATAAAATGGGGGGAGCGGCgggg                              >  2:415725/1‑144 (MQ=255)

                                                                                                                           tAATCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCATTa                       <  3:1366846/150‑1 (MQ=255)

                                                                                                                              tCTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGG‑GGGG‑GGGTAC‑TTTTTG‑TTTAACAAAACGGTGGGGGACATC‑AACCCCGG‑AAATAAAAATGGG‑GGACTggggggggggtattt                      >  3:832717/1‑139 (MQ=255)

                                                                                                                               cTTCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGGA‑GGGTAC‑TTTTTG‑TTTAACAAAGCGATGGGGACCCTC‑AA‑CCCCG‑AAATTAAAATTGGTGTACTGGCgggggttaatt                       >  2:1279163/1‑138 (MQ=255)

                                                                                                                                ttCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGC‑GGGA‑GGGTAA‑TTTTTG‑TTAAACAAAGCGATGGGGGCCATC‑AA‑CCCAG‑AAATTAAAATGGGGGTACTGGCgggggggattttac                    >  4:325376/1‑137 (MQ=255)

                                                                                                                                 tCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCATTACCg                    <  4:466224/147‑1 (MQ=255)

                                                                                                                                 tCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCATTACCg                    <  1:460335/147‑1 (MQ=255)

                                                                                                                                 tCGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGC‑GGGA‑GGGGTA‑TTTTTG‑TTTAACAAAACGGTGGGGACCAAC‑CA‑ACCAG‑AAATTAAAAAAGGGGGACTggggggggtgcattccc                    >  3:257128/1‑136 (MQ=255)

                                                                                                                                  cGCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCATTACCg                    <  3:243244/146‑1 (MQ=255)

                                                                                                                                   gCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGG‑GGGA‑GGGTAC‑TTTGTG‑TTTAACAAAAAGATGGGGGCCATC‑AA‑CCCCG‑AAATTAAAATGGGGGGAATGGCgggggggttttcac                    >  4:153648/1‑134 (MQ=255)

                                                                                                                                   gCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCATTACCGGTCtgg              <  3:739380/151‑1 (MQ=255)

                                                                                                                                   gCCATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGGTAC‑TTTTTG‑TTTACCAAAACGATGGGGACCATC‑AA‑CCCCG‑AAAATAACATTGGGGTACTGGCgggggtaactttcc                    >  1:453981/1‑134 (MQ=255)

                                                                                                                                    ccATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCATTACCGGTCtggt             <  3:728935/151‑1 (MQ=255)

                                                                                                                                     cATCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCATTACCGGTCtggtt            <  2:453556/151‑1 (MQ=255)

                                                                                                                                      aTCGGTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGTT‑GGGTTC‑ATTTTG‑TTAAACAAAACGGAGGGGGCCAAC‑CA‑ACCCG‑AAAATAAAACGGGGGGACTCGCggggagtagctccc                    >  4:334328/1‑131 (MQ=255)

                                                                                                                                       tCGGTGTGGCATTCAGCTGGGCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGGGG‑GGTTAC‑TTT‑TG‑TTAAACAAAACGGTGGGGGGCATC‑CA‑CCCAC‑AAAATAAAATGGGTGGATTTGCggggggttgcttacacc                 >  3:1306142/1‑130 (MQ=255)

                                                                                                                                         ggTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCATTACCGGTCTGGTtggtg        <  3:1068092/151‑1 (MQ=255)

                                                                                                                                         ggTGTGGCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGGGG‑GGGA‑GGGTAC‑TTTTTG‑TTAAACAAAGCGGTGGTGGCCCTC‑CA‑CCCAG‑AAATTTACAATGGGGTACTggggggggtgatttatcc                   >  4:4260/1‑128 (MQ=255)

                                                                                                                                              ggCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCATTACCGGTCTGGTTGGTGGCGCa   <  2:908405/151‑1 (MQ=255)

                                                                                                                                               gCATTCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCATTACCGGTCTGGTTGGTGGCGCAg  <  1:326631/151‑1 (MQ=255)

CGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGC‑GGTA‑GGTTAC‑TTTGTG‑TTAACCAAAGCGATGGTGACCATC‑AA‑CCCAG‑AAATTAACATGGGTGTACTGGCGGGTATCATTACCGGTCTGGTTGGTGGCGCAG  >  NC_000913/703981‑704274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`