Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,068,545	A→G	V432A (GTT→GCT)	rutG ←	pyrimidine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,068,545	0	A	G	100.0%	337.6 / NA	99	V432A (GTT→GCT)	rutG	pyrimidine permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/49);  total (50/49)

TATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTA  >  NC_000913/1068398‑1068691                                                                                                                                                     |                                                                                                                                                   tatTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGGGGAGCg                                                                                                                                                  >  2:343198/1‑150 (MQ=255)    tGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTca                                                                                                                                               >  2:12845/1‑150 (MQ=255)        tttGCACTTTTTTGTAGGCCGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTcaacca                                                                                                                                           <  3:322530/150‑1 (MQ=255)        tttGCACTGTTTTGTAGGCCGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTcaacc                                                                                                                                            >  3:1328570/1‑149 (MQ=255)        tttGCACTGTTTTGTAGGCCGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCCCCTGATGAACAACTTCTGGCGGTGGAGCGGcaacc                                                                                                                                            >  3:1367083/1‑149 (MQ=255)         ttGCACTGTTTTGTAGGCCGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTcaaccaa                                                                                                                                          <  3:233052/150‑1 (MQ=255)            cACTGTTTTGTAGGCCGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCCACCAAAttt                                                                                                                                      >  2:806663/1‑151 (MQ=255)               ttttttGTAGCCGGAATAGGGCGTTT‑GCGCCGCATCCGGAAAAAACAATGCGTACGTTGTCAAAACTCTGCCCCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTcaaccaac                                                                                                                                         <  2:113551/143‑1 (MQ=255)                 ttttGTAGGCCGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCt                                                                                                                                 >  1:220290/1‑151 (MQ=255)                 ttttGTAGGCCGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAAGGCGTACGTTGTAAACAATCGGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTc                                                                                                                                     <  2:734519/147‑1 (MQ=255)                    tGTAGGCGGGATAAGGCGTTT‑CCGCCACATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTa                                                                                                                               <  2:1100780/150‑1 (MQ=255)                     gaaGGCAGGATAA‑GCGTTTAACGCCACATCCGGCATAAAAAAGGCTAACGTTATCAACAATCTGAACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTa                                                                                                                               <  2:816401/147‑1 (MQ=255)                        ggCCGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGACAACCAACTTTCTGCTTaacaa                                                                                                                           <  1:366107/150‑1 (MQ=255)                           cGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGc                                                                                                                        >  4:168779/1‑150 (MQ=255)                            ggATAAGGCGTTT‑CCGCCGCATCCGGCATGAACAATGCGTCCGTTGTCAACAATCTCCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCa                                                                                                                       <  3:82682/150‑1 (MQ=255)                            ggATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCAt                                                                                                                      >  2:367798/1‑151 (MQ=255)                            ggATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGGGGAGCGTCAACCAACTTTCTGCTTAACAACGc                                                                                                                        >  3:1342279/1‑149 (MQ=255)                            ggATAAGGCGTTT‑ACGCCGCATCCGGCATGAAAAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACg                                                                                                                         <  2:840362/148‑1 (MQ=255)                              aTAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAAAAACGCAtt                                                                                                                     >  4:283962/1‑150 (MQ=255)                                aaGGCGTTT‑CCGCCGCATCCGGAATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAg                                                                                                                  <  2:211525/151‑1 (MQ=255)                                aaGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACTATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTg                                                                                                                    >  2:1324632/1‑149 (MQ=255)                                     gTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGGGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTaaa                                                                                                              >  3:1377894/1‑150 (MQ=255)                                     gTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTaaa                                                                                                              >  4:11114/1‑150 (MQ=255)                                      ttt‑ACGCCGCGTCCGGCATGAACATGGCGTACGTTGTCAACATTCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTaaa                                                                                                              <  2:830277/149‑1 (MQ=255)                                      ttt‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTaaaa                                                                                                             >  2:1190490/1‑150 (MQ=255)                                       tt‑ACGCCGCAGCCGGCATAAACAATGCTTACTTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAAt                                                                                                            <  2:1302496/150‑1 (MQ=255)                                        t‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATcg                                                                                                          >  2:22715/1‑151 (MQ=255)                                         tccgccgcACCCGGCATGAACAATGCGTACTTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATc                                                                                                           <  2:1327175/148‑1 (MQ=255)                                           cgccgcATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATcgc                                                                                                         >  4:182108/1‑150 (MQ=255)                                           cgccgcACCCGGAATGAAAAATGCGTACGTTGTCAACAATCGGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATcg                                                                                                          <  1:270781/149‑1 (MQ=255)                                             ccgcATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATcgcg                                                                                                        <  3:281450/149‑1 (MQ=255)                                             ccgcATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGcc                                                                                                      <  1:168782/151‑1 (MQ=255)                                             ccgcACCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATcgcg                                                                                                        <  1:351395/149‑1 (MQ=255)                                              cgcATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGcc                                                                                                      >  3:781124/1‑150 (MQ=255)                                              cgcATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCGCCTGATGAACAACTTCTGGCGGGGGAGGGTCAACCAACTTTCTGCTTAACAACGCATTGGGTAAAATCGCGcc                                                                                                      >  3:180806/1‑150 (MQ=255)                                                cATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCaa                                                                                                    >  4:462364/1‑150 (MQ=255)                                                cATCCGCCATAAACAATGCGTACTTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCaa                                                                                                    <  3:1304382/150‑1 (MQ=255)                                                   ccGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAgg                                                                                                 >  2:1397211/1‑150 (MQ=255)                                                   ccGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGt                                                                                                <  3:680591/151‑1 (MQ=255)                                                   ccGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAAATTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAgg                                                                                                 >  3:636600/1‑150 (MQ=255)                                                     ggCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTg                                                                                              <  2:242984/151‑1 (MQ=255)                                                     ggCATGAACAATGCGTACGTTGTCAACAATCGGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTg                                                                                              <  3:226448/151‑1 (MQ=255)                                                       cATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCt                                                                                            >  1:453684/1‑151 (MQ=255)                                                           aaaaaTGCTTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTa                                                                                         <  3:22720/147‑1 (MQ=255)                                                              aaTGCGTCCGTTGTCAACAATCGGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCa                                                                                      <  2:1367014/150‑1 (MQ=255)                                                              aaTGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGGGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCa                                                                                      >  2:696047/1‑150 (MQ=255)                                                              aaTGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCa                                                                                      >  2:237943/1‑150 (MQ=255)                                                               aTGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAAt                                                                                    <  1:93275/151‑1 (MQ=255)                                                                 gCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTCTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAAACCACTTTCTGCTTAAAAAAGCATTGCGGAAAATCGAGCCAAAGGTTGCTGTACCCAt                                                                                    >  2:730563/1‑149 (MQ=255)                                                                   gTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAAtccc                                                                                 <  3:246398/150‑1 (MQ=255)                                                                   gTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAAtccc                                                                                 >  3:894151/1‑150 (MQ=255)                                                                     aCGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAAtccctc                                                                               <  2:1373745/150‑1 (MQ=255)                                                                      cGTGGTCAACAATCGCCCCCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAAtccctc                                                                               <  3:1326575/149‑1 (MQ=255)                                                                        ttGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGGTGAACAACTTCTGGCGGGGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCa                                                                            >  4:336912/1‑150 (MQ=255)                                                                        ttGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAAtccctccc                                                                             <  1:423927/149‑1 (MQ=255)                                                                        ttGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAAtccctccc                                                                             <  2:726335/149‑1 (MQ=255)                                                                          gTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAAACAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCaa                                                                           >  4:155758/1‑149 (MQ=255)                                                                            caacaaTCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGGTGCTGTACCAATCCCTCCCAACg                                                                         >  3:508977/1‑149 (MQ=255)                                                                             aacaaTCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTa                                                                       <  3:12849/150‑1 (MQ=255)                                                                             aacaaGCTGAGCCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCaa                                                                           <  1:308267/146‑1 (MQ=255)                                                                              acaaTCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTaa                                                                      >  1:224876/1‑150 (MQ=255)                                                                                aaTCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGCACAACTTCTGGCGGTGGGGCGTCAACCCACTTTCTGCTTAACAAAGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCCAACCCTCCCAACGTaaaa                                                                    >  4:128430/1‑150 (MQ=255)                                                                                 aTCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAAcc                                                                  >  3:373139/1‑151 (MQ=255)                                                                                 aTCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAAcc                                                                  >  3:1216122/1‑151 (MQ=255)                                                                                    tGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGGTGCTGTACCAATCCCTCCCAACGTAAAACCGcc                                                               >  2:1091532/1‑151 (MQ=255)                                                                                      cACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGccc                                                              <  2:1089646/150‑1 (MQ=255)                                                                                       aCCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCa                                                             <  2:197987/150‑1 (MQ=255)                                                                                        ccgccgGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCa                                                             >  3:550419/1‑149 (MQ=255)                                                                                        ccgccgGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGc                                                           <  3:686432/151‑1 (MQ=255)                                                                                          gccgGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGt                                                         >  1:371423/1‑151 (MQ=255)                                                                                            cgGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGGGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTg                                                        >  2:772482/1‑150 (MQ=255)                                                                                                   cccGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCaaa                                                <  3:293031/151‑1 (MQ=255)                                                                                                    ccGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCaaaa                                               <  3:1059312/151‑1 (MQ=255)                                                                                                      ggcggTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATc                                             >  3:1180754/1‑151 (MQ=255)                                                                                                          gTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCAccc                                         >  3:877871/1‑151 (MQ=255)                                                                                                             ttCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCcgc                                       <  1:7694/150‑1 (MQ=255)                                                                                                                tGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGccc                                   >  3:1076286/1‑151 (MQ=255)                                                                                                                   ttATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAg                                 <  2:361590/150‑1 (MQ=255)                                                                                                                   ttATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAg                                 <  2:291985/150‑1 (MQ=255)                                                                                                                   ttATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGc                                >  2:472098/1‑151 (MQ=255)                                                                                                                    tATGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGc                                >  2:797969/1‑150 (MQ=255)                                                                                                                     aTGGCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCAc                              >  2:248919/1‑151 (MQ=255)                                                                                                                       ggCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACt                             >  3:681317/1‑150 (MQ=255)                                                                                                                       ggCTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTa                            <  1:209238/151‑1 (MQ=255)                                                                                                                         cTCCTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTa                            >  1:47585/1‑149 (MQ=255)                                                                                                                           ccTGATGAACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGt                        <  3:806698/151‑1 (MQ=255)                                                                                                                             tgatgaACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGtt                       >  4:298254/1‑150 (MQ=255)                                                                                                                              gatgaACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTa                      <  2:504190/150‑1 (MQ=255)                                                                                                                               atgaACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACt                    >  2:330297/1‑151 (MQ=255)                                                                                                                                 gaACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTg                   <  3:367830/150‑1 (MQ=255)                                                                                                                                 gaACAACTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGc                  >  2:422073/1‑151 (MQ=255)                                                                                                                                     aacTTCTGGCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGAcc              >  3:1260903/1‑151 (MQ=255)                                                                                                                                            ggCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATaa          <  2:825466/148‑1 (MQ=255)                                                                                                                                            ggCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAAt         <  3:1397282/149‑1 (MQ=255)                                                                                                                                            ggCGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCa       <  2:1328499/151‑1 (MQ=255)                                                                                                                                              cGGTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCaaa     <  3:1324702/151‑1 (MQ=255)                                                                                                                                                gTGGAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAAtt   >  1:177591/1‑151 (MQ=255)                                                                                                                                                  ggAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTa  <  2:921559/150‑1 (MQ=255)                                                                                                                                                  ggAGCGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTa  <  3:1190547/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTT‑ACGCCGCATCCGGCATGAACAATGCGTACGTTGTCAACAATCTGCACCGCCGGTAACCCCGGCGGTTTTCTGTTTATGGCTCCTGATGAACAACTTCTGGCGGTGGAACGTCAACCAACTTTCTGCTTAACAACGCATTGAGTAAAATCGCGCCAAAGGTTGCTGTACCAATCCCTCCCAACGTAAAACCGCCCAGCGTGAGAGCAAAATCACCCGCGCCCAGCACTAAGGTTACTGCGACCATAATCAAATTA  >  NC_000913/1068398‑1068691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑