Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 134,458 T→C V24A (GTC→GCC)  yacL → UPF0231 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913134,4580TC99.2% 469.7 / ‑4.0 128V24A (GTC→GCC) yacLUPF0231 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (58/69);  total (58/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 128 total aligned reads displayed.

TTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGAT  >  NC_000913/134310‑134605
                                                                                                                                                    |                                                                                                                                                   
ttCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCg                                                                                                                                                   >  4:403830/1‑151 (MQ=255)
 tctcCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCgg                                                                                                                                                  >  2:17618/1‑151 (MQ=255)
    ccGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCggg                                                                                                                                                 <  4:394855/149‑1 (MQ=255)
     cGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCAc                                                                                                                                              >  2:282089/1‑151 (MQ=255)
      ggTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACt                                                                                                                                             <  2:293342/151‑1 (MQ=255)
       gTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTg                                                                                                                                            >  2:1332368/1‑151 (MQ=255)
        tAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTgg                                                                                                                                           >  3:1183521/1‑151 (MQ=255)
         aaGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGt                                                                                                                                          >  2:231537/1‑151 (MQ=255)
         aaGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGt                                                                                                                                          >  1:402109/1‑151 (MQ=255)
         aaGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGt                                                                                                                                          >  3:153217/1‑151 (MQ=255)
          aGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGtt                                                                                                                                         <  2:1155793/151‑1 (MQ=255)
               gCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATg                                                                                                                                    <  3:937213/151‑1 (MQ=255)
               gCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATg                                                                                                                                    >  3:1262599/1‑151 (MQ=255)
               gCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATg                                                                                                                                    <  3:193769/151‑1 (MQ=255)
                cGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATg                                                                                                                                    >  3:1021447/1‑150 (MQ=255)
                cGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGa                                                                                                                                   <  1:141485/151‑1 (MQ=255)
                cGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGa                                                                                                                                   <  4:338955/151‑1 (MQ=255)
                  aTAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAag                                                                                                                                 <  2:384946/151‑1 (MQ=255)
                     aTTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAgg                                                                                                                              >  2:618018/1‑151 (MQ=255)
                     aTTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAgg                                                                                                                              <  3:972439/151‑1 (MQ=255)
                        aCATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTg                                                                                                                            <  2:12385/150‑1 (MQ=255)
                            cAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTgaa                                                                                                                          >  2:46330/1‑148 (MQ=255)
                               tGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGGGGTgaaagaaa                                                                                                                     >  3:23227/1‑150 (MQ=255)
                                ggCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGaaaa                                                                                                                    <  3:1139490/150‑1 (MQ=255)
                                ggCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGaaaa                                                                                                                    <  2:134155/150‑1 (MQ=255)
                                ggCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAAc                                                                                                                   <  3:1153327/151‑1 (MQ=255)
                                ggCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAAc                                                                                                                   <  3:639276/151‑1 (MQ=255)
                                 gcgcAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAAcc                                                                                                                  <  2:91036/151‑1 (MQ=255)
                                  cgcAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCt                                                                                                                 <  3:357150/151‑1 (MQ=255)
                                   gcAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTg                                                                                                                >  3:520108/1‑151 (MQ=255)
                                   gcAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTg                                                                                                                <  3:794082/151‑1 (MQ=255)
                                        gCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCctt                                                                                                           <  3:441191/151‑1 (MQ=255)
                                        gCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCctt                                                                                                           <  2:1239694/151‑1 (MQ=255)
                                          gATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttgc                                                                                                         <  3:898122/151‑1 (MQ=255)
                                             ttCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttgctt                                                                                                       <  1:358155/150‑1 (MQ=255)
                                             ttCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGAGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttgctt                                                                                                       >  1:240573/1‑150 (MQ=255)
                                               cGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtga                                                                                                     <  2:1087132/150‑1 (MQ=255)
                                                   gCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtgatga                                                                                                  >  4:411306/1‑149 (MQ=255)
                                                     aTTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTg                                                                                              >  3:23037/1‑151 (MQ=255)
                                                     aTTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTg                                                                                              >  3:1254814/1‑151 (MQ=255)
                                                          cGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGAGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAAc                                                                                          >  2:1015249/1‑150 (MQ=255)
                                                           gggAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACaa                                                                                        <  1:185374/151‑1 (MQ=255)
                                                             gAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAagc                                                                                      >  2:199117/1‑151 (MQ=255)
                                                                cAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAagcagc                                                                                   >  3:273646/1‑151 (MQ=255)
                                                                cAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAagcagc                                                                                   <  1:475261/151‑1 (MQ=255)
                                                                 agagGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAgcg                                                                                  >  4:332425/1‑151 (MQ=255)
                                                                  gagGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAgcgc                                                                                 <  2:559582/151‑1 (MQ=255)
                                                                   agGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgca                                                                                <  2:344022/151‑1 (MQ=255)
                                                                     gAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgcacg                                                                              <  2:947/151‑1 (MQ=255)
                                                                     gAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgcacg                                                                              <  3:607800/151‑1 (MQ=255)
                                                                      aaCACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgcacgc                                                                             <  3:817795/151‑1 (MQ=255)
                                                                       acacacTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgcacgca                                                                            <  2:305839/151‑1 (MQ=255)
                                                                       acacacTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgcacgc                                                                             >  4:365874/1‑150 (MQ=255)
                                                                         acacTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACt                                                                          >  1:9032/1‑151 (MQ=255)
                                                                             tATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGaa                                                                       >  3:659918/1‑150 (MQ=255)
                                                                                ggATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGt                                                                   <  1:266771/151‑1 (MQ=255)
                                                                                 gATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTa                                                                  <  2:420543/151‑1 (MQ=255)
                                                                                    tACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCg                                                               >  1:154689/1‑151 (MQ=255)
                                                                                     aCGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCg                                                               >  3:624701/1‑150 (MQ=255)
                                                                                       gAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAAc                                                            <  1:117870/151‑1 (MQ=255)
                                                                                       gAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAAc                                                            <  2:520073/151‑1 (MQ=255)
                                                                                        aaTTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACg                                                           >  3:1257937/1‑151 (MQ=255)
                                                                                         aTTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCAAGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACgg                                                          >  2:1086817/1‑151 (MQ=255)
                                                                                           ttCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTc                                                        >  3:1182491/1‑151 (MQ=255)
                                                                                             cTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCt                                                      >  2:1334864/1‑151 (MQ=255)
                                                                                               gcgcGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTcc                                                       <  3:1357343/148‑1 (MQ=255)
                                                                                                cgcgATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGc                                                   >  2:536192/1‑151 (MQ=255)
                                                                                                cgcgATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGc                                                   <  1:469534/151‑1 (MQ=255)
                                                                                                    atatTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACg                                               >  3:1353205/1‑151 (MQ=255)
                                                                                                        tACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggc                                            <  3:17624/150‑1 (MQ=255)
                                                                                                          ccGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcag                                          <  3:29215/150‑1 (MQ=255)
                                                                                                             gAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcagggca                                      <  3:46341/151‑1 (MQ=255)
                                                                                                                 ggTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGa                                   >  3:229993/1‑150 (MQ=255)
                                                                                                                  gTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGaa                                  >  3:1391003/1‑150 (MQ=255)
                                                                                                                    aaaGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATa                                <  3:1015355/150‑1 (MQ=255)
                                                                                                                    aaaGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATa                                <  2:1183465/150‑1 (MQ=255)
                                                                                                                     aaGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATac                               >  3:398795/1‑150 (MQ=255)
                                                                                                                        gTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACAccc                           <  3:1201319/151‑1 (MQ=255)
                                                                                                                          gCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCtg                         >  2:1155457/1‑151 (MQ=255)
                                                                                                                          gCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCtg                         >  3:652777/1‑151 (MQ=255)
                                                                                                                           cGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCtgt                        <  2:1281513/151‑1 (MQ=255)
                                                                                                                              aTGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtgga                     <  2:1021403/151‑1 (MQ=255)
                                                                                                                              aTGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtgg                      >  1:241587/1‑150 (MQ=255)
                                                                                                                                gTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggat                    <  2:1262533/150‑1 (MQ=255)
                                                                                                                                gTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggat                    <  2:1390933/150‑1 (MQ=255)
                                                                                                                                 tCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgg                  >  3:1342358/1‑151 (MQ=255)
                                                                                                                                  ccATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgga                 <  2:23222/151‑1 (MQ=255)
                                                                                                                                  ccATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgg                  >  2:943768/1‑150 (MQ=255)
                                                                                                                                  ccATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgg                  >  2:1077682/1‑150 (MQ=255)
                                                                                                                                  ccATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgg                  <  3:282115/150‑1 (MQ=255)
                                                                                                                                   cATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatgga                 <  2:181318/150‑1 (MQ=255)
                                                                                                                                      ggggCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACgg              <  3:442134/150‑1 (MQ=255)
                                                                                                                                      ggggCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACgg              >  2:1032666/1‑150 (MQ=255)
                                                                                                                                      ggggCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCACACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGt             <  4:378899/151‑1 (MQ=255)
                                                                                                                                       gggCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTg            >  3:94962/1‑151 (MQ=255)
                                                                                                                                           aTGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGa           >  2:140746/1‑148 (MQ=255)
                                                                                                                                           aTGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGa           >  2:1185467/1‑148 (MQ=255)
                                                                                                                                             gAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAagag       <  3:884577/150‑1 (MQ=255)
                                                                                                                                               aGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTg    >  3:112507/1‑151 (MQ=255)
                                                                                                                                               aGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTg    <  3:1332441/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGAT  >  NC_000913/134310‑134605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑