Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,449,092 A→G L753P (CTG→CCG)  tynA ← tyramine oxidase, copper‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,449,0920AG99.0% 368.3 / NA 97L753P (CTG→CCG) tynAtyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (53/43);  total (54/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTCC  >  NC_000913/1448946‑1449238
                                                                                                                                                  |                                                                                                                                                  
aaCGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGgcgc                                                                                                                                                <  2:1101636/151‑1 (MQ=255)
   gggCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGcccc                                                                                                                                             >  2:740213/1‑151 (MQ=255)
      ccGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCt                                                                                                                                            >  1:170097/1‑149 (MQ=255)
          gATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGt                                                                                                                                       <  4:304403/150‑1 (MQ=255)
           aTTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTg                                                                                                                                     <  2:791674/151‑1 (MQ=255)
           aTTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTg                                                                                                                                     >  2:475660/1‑151 (MQ=255)
              ttGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGc                                                                                                                                   <  3:334030/150‑1 (MQ=255)
              ttGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCg                                                                                                                                  >  2:87840/1‑151 (MQ=255)
               tGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCg                                                                                                                                  <  2:27194/150‑1 (MQ=255)
               tGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGt                                                                                                                                 >  3:654679/1‑151 (MQ=255)
                ggCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGtt                                                                                                                                <  3:351481/151‑1 (MQ=255)
                 gCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAGCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAAGCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGttt                                                                                                                               <  1:194897/151‑1 (MQ=255)
                 gCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGttt                                                                                                                               <  3:410151/151‑1 (MQ=255)
                  ccccGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTc                                                                                                                              >  1:314962/1‑151 (MQ=255)
                   cccGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCg                                                                                                                             >  3:1007159/1‑151 (MQ=255)
                   cccGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCg                                                                                                                             >  3:772696/1‑151 (MQ=255)
                    ccGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGt                                                                                                                            <  3:368925/151‑1 (MQ=255)
                      gACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCa                                                                                                                          <  3:1081979/151‑1 (MQ=255)
                       aCTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTc                                                                                                                           >  2:1873/1‑149 (MQ=255)
                       aCTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCa                                                                                                                          >  3:459777/1‑150 (MQ=255)
                       aCTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCa                                                                                                                          >  1:445421/1‑150 (MQ=255)
                         tggctggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCaa                                                                                                                         >  3:133952/1‑149 (MQ=255)
                          ggctggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCaaa                                                                                                                        <  2:1221784/149‑1 (MQ=255)
                           gctggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTCAAAAACGGCTCCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAaagaa                                                                                                                     <  3:150499/151‑1 (MQ=255)
                             tggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGt                                                                                                                   >  2:95906/1‑151 (MQ=255)
                              ggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGtt                                                                                                                  <  2:414054/151‑1 (MQ=255)
                              ggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGtt                                                                                                                  <  2:882408/150‑1 (MQ=255)
                                  ggTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCAt                                                                                                              <  2:1325272/151‑1 (MQ=255)
                                  ggTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCAt                                                                                                              >  3:312991/1‑151 (MQ=255)
                                  ggTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCAt                                                                                                              >  1:103010/1‑151 (MQ=255)
                                   gTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATg                                                                                                             <  4:55727/151‑1 (MQ=255)
                                     tGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGt                                                                                                           >  1:155421/1‑151 (MQ=255)
                                      ggTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGtt                                                                                                          <  2:698287/151‑1 (MQ=255)
                                      ggTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGt                                                                                                           <  4:415785/150‑1 (MQ=255)
                                         gtgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTc                                                                                                        <  3:218933/150‑1 (MQ=255)
                                              aCTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCaga                                                                                                  <  2:759967/151‑1 (MQ=255)
                                                 aaGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGt                                                                                                <  3:1873/150‑1 (MQ=255)
                                                    tCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGt                                                                                            >  2:625914/1‑151 (MQ=255)
                                                     cGGTGTGTGTACGGTCTTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGt                                                                                            >  4:47395/1‑150 (MQ=255)
                                                     cGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGt                                                                                            <  3:535336/150‑1 (MQ=255)
                                                         gtgtgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCa                                                                                       <  1:477603/151‑1 (MQ=255)
                                                             gtACGGTATTAATCTGATTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTACGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATAGTTTCAGCAGAGTATGTACCCATtcg                                                                                   <  3:1400010/151‑1 (MQ=255)
                                                              tACTGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCAtt                                                                                     <  4:85995/148‑1 (MQ=255)
                                                               aCGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcga                                                                                  <  4:252102/150‑2 (MQ=255)
                                                                cGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcggtc                                                                                <  2:606420/151‑1 (MQ=255)
                                                                  gTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcggtcgg                                                                              >  1:204568/1‑151 (MQ=255)
                                                                   tATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcagc                                                                                                     <  1:309533/127‑1 (MQ=255)
                                                                   tATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcagc                                                                                                     >  4:309530/1‑127 (MQ=255)
                                                                   tATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcggtcg                                                                               >  4:82470/1‑149 (MQ=255)
                                                                    aTTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGc                                                                             >  2:462904/1‑150 (MQ=255)
                                                                    aTTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCa                                                                            <  3:384116/151‑1 (MQ=255)
                                                                       aaTCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATa                                                                          <  2:1145476/150‑1 (MQ=255)
                                                                            ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATc                                                                       <  2:434555/148‑1 (MQ=255)
                                                                            ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCgg                                                                     <  1:76971/150‑1 (MQ=255)
                                                                            ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCg                                                                      <  2:185791/149‑1 (MQ=255)
                                                                            ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGc                                                                    >  4:304803/1‑151 (MQ=255)
                                                                              ttCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTCTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGcc                                                                   >  2:659764/1‑150 (MQ=255)
                                                                                cGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCAct                                                                >  1:413631/1‑151 (MQ=255)
                                                                                   tCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCtt                                                             >  2:864226/1‑151 (MQ=255)
                                                                                    cATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTc                                                            <  4:42909/151‑1 (MQ=255)
                                                                                    cATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTc                                                            >  3:1259507/1‑151 (MQ=255)
                                                                                     aTAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCg                                                           >  2:979332/1‑151 (MQ=255)
                                                                                      tAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCgg                                                          >  2:1244017/1‑151 (MQ=255)
                                                                                       aaGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGgc                                                         >  4:218201/1‑151 (MQ=255)
                                                                                          tAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGcgcg                                                      >  4:79840/1‑151 (MQ=255)
                                                                                          tAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGcgcg                                                      >  3:680745/1‑151 (MQ=255)
                                                                                             aaaaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGc                                                   >  2:952524/1‑151 (MQ=255)
                                                                                              aaaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGcc                                                  >  3:1312902/1‑151 (MQ=255)
                                                                                               aaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCca                                                 <  1:322037/151‑1 (MQ=255)
                                                                                                   gCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACAtg                                             <  2:459742/151‑1 (MQ=255)
                                                                                                    cACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACAtgt                                            >  3:144766/1‑151 (MQ=255)
                                                                                                     aCCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACAtgtg                                           >  2:109630/1‑151 (MQ=255)
                                                                                                     aCCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACAtgtg                                           >  3:175207/1‑151 (MQ=255)
                                                                                                         ggTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACAtgtgtg                                         <  2:1007117/149‑1 (MQ=255)
                                                                                                         ggTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTgtggt                                       <  4:162516/151‑1 (MQ=255)
                                                                                                           tGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGGGGTGc                                     >  2:383334/1‑151 (MQ=255)
                                                                                                              cGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCgg                                  >  3:1084386/1‑151 (MQ=255)
                                                                                                              cGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCgg                                  >  4:460961/1‑151 (MQ=255)
                                                                                                              cGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCgg                                  >  2:454808/1‑151 (MQ=255)
                                                                                                               gTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCgg                                  >  2:1206835/1‑150 (MQ=255)
                                                                                                                 ttttttGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCggtg                                <  2:582753/150‑1 (MQ=255)
                                                                                                                  tttttGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCggtggt                              >  2:585792/1‑151 (MQ=255)
                                                                                                                      tGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATc                          >  4:181496/1‑151 (MQ=255)
                                                                                                                      tGTCTGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATc                          >  3:600953/1‑151 (MQ=255)
                                                                                                                          tGAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATcc                         >  2:991487/1‑148 (MQ=255)
                                                                                                                           gAAACAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTTGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCa                        <  2:997574/148‑1 (MQ=255)
                                                                                                                             aaCAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACa                    >  3:126392/1‑150 (MQ=255)
                                                                                                                             aaCAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACa                    >  3:916686/1‑150 (MQ=255)
                                                                                                                               cAATCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACACTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACg                 >  4:353069/1‑151 (MQ=255)
                                                                                                                                aaTCACTTATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACgg                >  3:981332/1‑151 (MQ=255)
                                                                                                                                       tATCTTTCTTCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCg           <  2:1281611/149‑1 (MQ=255)
                                                                                                                                          ctttcttCGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGtgtt      >  3:949762/1‑151 (MQ=255)
                                                                                                                                            ttcttcGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGgtg        <  2:1079289/147‑1 (MQ=255)
                                                                                                                                            ttcttcGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGtgttgt    >  3:368800/1‑151 (MQ=255)
                                                                                                                                             tcttcGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGtgttgt    <  3:106301/150‑1 (MQ=255)
                                                                                                                                              cttcGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTcc  >  3:578400/1‑151 (MQ=255)
                                                                                                                                               ttcGGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTcc  >  2:567130/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTGTGGTGCCGGTGGTCATCCAGACAACGGCGTCGGTGTTGTCC  >  NC_000913/1448946‑1449238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: