Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,464,068 T→C L177P (CTT→CCT)  paaX → transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,464,0680TC100.0% 407.0 / NA 107L177P (CTT→CCT) paaXtranscriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/60);  total (47/60)

Only 100 of 107 total aligned reads displayed.

CAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATT  >  NC_000913/1463923‑1464215
                                                                                                                                                 |                                                                                                                                                   
cAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTtctc                                                                                                                                                <  2:363355/151‑1 (MQ=255)
 aGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTtctc                                                                                                                                                >  1:440938/1‑150 (MQ=255)
 aGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTcg                                                                                                                                               <  2:641747/151‑1 (MQ=255)
 aGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTcg                                                                                                                                               <  2:324283/151‑1 (MQ=255)
    tGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTcgcg                                                                                                                                             <  3:819983/150‑1 (MQ=255)
     gATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGTAGCGCAAATACCACAGGCGCCTTCTcg                                                                                                                                               >  2:166439/1‑147 (MQ=255)
     gATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTcgc                                                                                                                                              >  4:154756/1‑148 (MQ=255)
      aTCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGGGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCgca                                                                                                                                           >  3:1216826/1‑150 (MQ=255)
       tCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGAAAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCgcag                                                                                                                                          >  3:1366511/1‑150 (MQ=255)
          ggCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCAc                                                                                                                                       <  3:166463/150‑1 (MQ=255)
              aGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCg                                                                                                                                   >  1:218209/1‑150 (MQ=255)
                    tGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGcc                                                                                                                               >  2:1175175/1‑148 (MQ=255)
                     ggCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCaga                                                                                                                            <  3:305220/150‑1 (MQ=255)
                       cgcACTGGCACCCAGCTTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTa                                                                                                                         <  1:289385/151‑1 (MQ=255)
                        gcACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTag                                                                                                                        <  1:124817/151‑1 (MQ=255)
                        gcACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTag                                                                                                                        <  4:241501/151‑1 (MQ=255)
                         cACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTaga                                                                                                                       <  3:1164429/151‑1 (MQ=255)
                         cACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTaga                                                                                                                       <  3:88267/151‑1 (MQ=255)
                           cTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTaga                                                                                                                       >  3:333492/1‑149 (MQ=255)
                            tGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTaga                                                                                                                       >  2:307595/1‑148 (MQ=255)
                             ggCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAagag                                                                                                                   <  2:109277/151‑1 (MQ=255)
                               cACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTg                                                                                                                 >  3:800462/1‑151 (MQ=255)
                               cACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTg                                                                                                                 >  3:1103111/1‑151 (MQ=255)
                                    aGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGc                                                                                                            <  2:628976/151‑1 (MQ=255)
                                    aGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGGGCTgg                                                                                                             >  3:596663/1‑150 (MQ=255)
                                       cTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCAtt                                                                                                         >  4:299529/1‑151 (MQ=255)
                                         gATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTa                                                                                                       <  2:120802/151‑1 (MQ=255)
                                            ggCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACt                                                                                                    <  2:995254/151‑1 (MQ=255)
                                            ggCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACt                                                                                                    <  3:437601/151‑1 (MQ=255)
                                                 cGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAAAGTGATTTGTTTTGAAGCGCAAATACCAATGGCGCCTTCTCGCGCAGCACTGCGTGGCAGAGGAGAAGAATGCTGGCATTTAACTGaa                                                                                                 >  3:860341/1‑149 (MQ=255)
                                                  gCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGaa                                                                                                 <  2:49625/148‑1 (MQ=255)
                                                  gCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGGACa                                                                                               >  2:909852/1‑150 (MQ=255)
                                                  gCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGCGGTTTGTTTTGAAACGCAAATACCACTGGCGCCTTCTAGCGGCACACTGCGTGACAGAGGAGAAGAGTGGTGGCATTTAACTGAACa                                                                                               >  2:857065/1‑150 (MQ=255)
                                                   ccGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGCTTTGTTTTGAAGCGCAAATACCACTGGGGCCTTCTCGCGCAGCACTGCGGGGCAGAGTAGAAAAGGGCCGGCATTTAACTGAAc                                                                                                >  4:279590/1‑148 (MQ=255)
                                                   ccGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACaaa                                                                                             <  4:218207/151‑1 (MQ=255)
                                                   ccGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACaaa                                                                                             <  1:211260/151‑1 (MQ=255)
                                                    cGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACaaaa                                                                                            <  2:1301427/151‑1 (MQ=255)
                                                     gTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACaaaa                                                                                            >  1:227243/1‑150 (MQ=255)
                                                        gCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGAGATTTTTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCCGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGcc                                                                                        >  4:303236/1‑151 (MQ=255)
                                                         cAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCa                                                                                       <  2:111935/151‑1 (MQ=255)
                                                          aaaaaCTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCAt                                                                                      >  3:256649/1‑151 (MQ=255)
                                                           aaaaCTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCAt                                                                                      >  3:110770/1‑150 (MQ=255)
                                                           aaaaCTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATg                                                                                     <  3:808141/151‑1 (MQ=255)
                                                            aaaCTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGt                                                                                    <  3:679666/151‑1 (MQ=255)
                                                              aCTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTAc                                                                                  <  1:98908/151‑1 (MQ=255)
                                                                 ggCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCCGAGGAGAAGAGTGCTGGCATTTAAATGAACAAAATGCCATGTa                                                                                   >  3:473965/1‑147 (MQ=255)
                                                                     gATGTACAGACACTTTTGCATGAAGCGGGCGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCCTGTACGAAAcc                                                                            >  2:918947/1‑150 (MQ=255)
                                                                      aTGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAAAGTGATTTGTTTTGAAGCGCAAAAACCACTGGGGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCtt                                                                          >  1:301966/1‑151 (MQ=255)
                                                                       tGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCttt                                                                         >  1:201313/1‑151 (MQ=255)
                                                                        gTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCttt                                                                         <  1:163258/150‑1 (MQ=255)
                                                                        gTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCttt                                                                         >  3:87379/1‑150 (MQ=255)
                                                                        gTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCttt                                                                         >  2:123712/1‑150 (MQ=255)
                                                                        gTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTa                                                                        <  3:554531/151‑1 (MQ=255)
                                                                        gTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTa                                                                        <  4:276033/151‑1 (MQ=255)
                                                                        gTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTa                                                                        <  2:1103061/151‑1 (MQ=255)
                                                                         tACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTAt                                                                       >  1:237854/1‑151 (MQ=255)
                                                                          aCAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTGCGAAACCTTTAtt                                                                      <  2:651325/151‑1 (MQ=255)
                                                                          aCAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTAt                                                                       <  2:824947/150‑1 (MQ=255)
                                                                           cAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTc                                                                     <  3:680614/151‑1 (MQ=255)
                                                                              acacTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGt                                                                  <  3:444707/151‑1 (MQ=255)
                                                                                acTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTc                                                                 >  2:158024/1‑150 (MQ=255)
                                                                                       cATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTccgcc                                                         >  2:179026/1‑151 (MQ=255)
                                                                                       cATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTccgcc                                                         <  1:73504/151‑1 (MQ=255)
                                                                                        aTGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTccgccc                                                        <  3:750089/151‑1 (MQ=255)
                                                                                          gAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCGGGCATTTAAATGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTccgcccgc                                                      >  1:422160/1‑151 (MQ=255)
                                                                                           aaGCGGGTGTGGCGGATAACGTGGTTTGTTTTGAAGCGCAAATACCACTGGCGCCCTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAAATGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTtcgcccgc                                                      >  3:97618/1‑150 (MQ=255)
                                                                                                ggTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGc                                                <  2:359072/151‑1 (MQ=255)
                                                                                                     ggCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCtt                                           <  2:100165/151‑1 (MQ=255)
                                                                                                       cGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCtttt                                         >  2:572802/1‑151 (MQ=255)
                                                                                                       cGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCtttt                                         >  4:403947/1‑151 (MQ=255)
                                                                                                            aaCGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTaaaa                                     <  4:43395/150‑1 (MQ=255)
                                                                                                             aCGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTaaa                                      >  4:233274/1‑148 (MQ=255)
                                                                                                              cGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAag                                    >  3:984932/1‑149 (MQ=255)
                                                                                                               gTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAagag                                  <  3:1176742/150‑1 (MQ=255)
                                                                                                               gTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAgg                                 <  3:440700/151‑1 (MQ=255)
                                                                                                               gTGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAgg                                 >  2:1215427/1‑151 (MQ=255)
                                                                                                                tGATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAggc                                >  3:971451/1‑151 (MQ=255)
                                                                                                                 gATTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAggcg                               <  3:1079154/151‑1 (MQ=255)
                                                                                                                  aTTTGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAggcgg                              <  3:844618/151‑1 (MQ=255)
                                                                                                                   tttGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAggcggc                             >  4:375145/1‑151 (MQ=255)
                                                                                                                    ttGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAggcggc                             >  3:731203/1‑150 (MQ=255)
                                                                                                                    ttGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAggcggc                             <  4:337250/150‑1 (MQ=255)
                                                                                                                    ttGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAggcggc                             >  3:1207218/1‑150 (MQ=255)
                                                                                                                    ttGTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGATTTTAAAAGAggcggc                             <  1:108994/150‑1 (MQ=255)
                                                                                                                      gTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAg                           <  2:1216765/150‑1 (MQ=255)
                                                                                                                      gTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAg                           <  1:399823/150‑1 (MQ=255)
                                                                                                                      gTTTTGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGa                          <  2:97603/151‑1 (MQ=255)
                                                                                                                        tttGAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGAc                         <  3:123727/150‑1 (MQ=255)
                                                                                                                           gAAGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAg                      <  4:311052/150‑1 (MQ=255)
                                                                                                                             aGCGCAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTa                   <  3:239067/151‑1 (MQ=255)
                                                                                                                              gcgcAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTaa                  <  2:1337909/151‑1 (MQ=255)
                                                                                                                                 cAAATACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCACGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAAccc               >  4:314031/1‑151 (MQ=255)
                                                                                                                                  aaaTACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAAcccc              <  1:467619/151‑1 (MQ=255)
                                                                                                                                  aaaTACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAAcccc              >  1:131808/1‑151 (MQ=255)
                                                                                                                                   aaTACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCg             >  1:159291/1‑151 (MQ=255)
                                                                                                                                     tACCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCgg            >  3:1060178/1‑150 (MQ=255)
                                                                                                                                      aCCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGa           <  2:87367/150‑1 (MQ=255)
                                                                                                                                      aCCACTGGCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGa           <  2:333464/150‑1 (MQ=255)
                                                                                                                                            ggCGCCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCa    <  3:1262035/151‑1 (MQ=255)
                                                                                                                                              cgcCTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCAtt  <  2:731172/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CAGTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCAGCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTTTGCATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGCAAATACCACTGGCGCTTTCTCGCGCAGCACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTATTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATT  >  NC_000913/1463923‑1464215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑