BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,496,777	T→C	intergenic (+146/‑79)	ydcJ → / → opgD	putative metalloenzyme/OPG biosynthetic periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,496,777	0	T	C	100.0%	441.8 / NA	112	intergenic (+146/‑79)	ydcJ/opgD	putative metalloenzyme/OPG biosynthetic periplasmic protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (61/51); total (61/51)

Only 100 of 112 total aligned reads displayed.

AAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTGCT  >  NC_000913/1496630‑1496924

aaaaTCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCa                                                                                                                                                   <  3:945088/150‑1 (MQ=255)

 aaaTCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCata                                                                                                                                                 <  3:408414/151‑1 (MQ=255)

  aaTCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCataa                                                                                                                                                <  3:939362/151‑1 (MQ=255)

  aaTCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCata                                                                                                                                                 >  3:634589/1‑150 (MQ=255)

       gACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCataaata                                                                                                                                             <  2:1369599/149‑1 (MQ=255)

           aTCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACt                                                                                                                                       >  4:416171/1‑151 (MQ=255)

           aTCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACt                                                                                                                                       >  3:802336/1‑151 (MQ=255)

              cGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTc                                                                                                                                    <  2:431944/151‑1 (MQ=255)

                ccTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGCTTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCtt                                                                                                                                  >  3:400158/1‑151 (MQ=255)

                  tttGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGc                                                                                                                                >  2:1075362/1‑151 (MQ=255)

                          aaTTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGaaa                                                                                                                        <  2:1104693/151‑1 (MQ=255)

                           aTTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGaaa                                                                                                                        >  3:79131/1‑150 (MQ=255)

                             ttGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAAc                                                                                                                      <  3:1019614/150‑1 (MQ=255)

                              tGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACg                                                                                                                     >  3:641589/1‑150 (MQ=255)

                                ccTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGAcc                                                                                                                  <  2:1271543/151‑1 (MQ=255)

                                ccTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGAcc                                                                                                                  <  3:634011/151‑1 (MQ=255)

                                  tGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGAcc                                                                                                                  >  1:308970/1‑149 (MQ=255)

                                    aTTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAgcg                                                                                                              >  3:1143904/1‑151 (MQ=255)

                                     ttttACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAgcgc                                                                                                             >  3:586343/1‑151 (MQ=255)

                                         aCAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCggg                                                                                                          >  4:327043/1‑150 (MQ=255)

                                         aCAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGa                                                                                                         <  2:951503/151‑1 (MQ=255)

                                          cAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGAc                                                                                                        >  3:320633/1‑151 (MQ=255)

                                            aaCGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGAcc                                                                                                       >  3:813277/1‑150 (MQ=255)

                                            aaCGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGAcc                                                                                                       >  3:451119/1‑150 (MQ=255)

                                              cGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCAtt                                                                                                    <  2:787015/151‑1 (MQ=255)

                                               gAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTc                                                                                                   >  2:1181346/1‑151 (MQ=255)

                                                    aGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCacaac                                                                                              >  2:120377/1‑151 (MQ=255)

                                                    aGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCacaac                                                                                              <  1:388668/151‑1 (MQ=255)

                                                       cTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACAcc                                                                                           >  4:407952/1‑151 (MQ=255)

                                                         cATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCa                                                                                          <  3:1168056/150‑1 (MQ=255)

                                                         cATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCa                                                                                          <  3:610096/150‑1 (MQ=255)

                                                         cATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCa                                                                                          <  2:1368266/150‑1 (MQ=255)

                                                         cATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAg                                                                                         <  2:62571/151‑1 (MQ=255)

                                                             ccATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAgg                                                                                     <  4:316768/151‑1 (MQ=255)

                                                              cATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGa                                                                                    <  2:813243/151‑1 (MQ=255)

                                                               aTCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGAc                                                                                   <  1:255008/151‑1 (MQ=255)

                                                               aTCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGAc                                                                                   <  2:800855/151‑1 (MQ=255)

                                                               aTCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGAc                                                                                   <  2:1200149/151‑1 (MQ=255)

                                                                     aTAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACtt                                                                             <  1:150613/151‑1 (MQ=255)

                                                                     aTAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACtt                                                                             >  2:1226028/1‑151 (MQ=255)

                                                                      tAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACttt                                                                            >  1:360849/1‑151 (MQ=255)

                                                                       aaaaaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTc                                                                           >  2:1058831/1‑151 (MQ=255)

                                                                        aaaaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCa                                                                          <  3:307179/151‑1 (MQ=255)

                                                                          aaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAgg                                                                        >  3:292254/1‑151 (MQ=255)

                                                                           aaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGt                                                                       >  2:363256/1‑151 (MQ=255)

                                                                            aaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGt                                                                       >  2:1309601/1‑150 (MQ=255)

                                                                             aaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTAt                                                                     >  1:255346/1‑151 (MQ=255)

                                                                             aaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTCCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTAt                                                                     <  4:160915/151‑1 (MQ=255)

                                                                              aTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATg                                                                    <  2:832654/151‑1 (MQ=255)

                                                                              aTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATg                                                                    <  3:1126131/151‑1 (MQ=255)

                                                                              aTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATg                                                                    <  3:1223252/151‑1 (MQ=255)

                                                                                     tttATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGt                                                              >  3:396554/1‑150 (MQ=255)

                                                                                      ttATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGt                                                              >  2:494772/1‑149 (MQ=255)

                                                                                       tATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGt                                                              >  1:83411/1‑148 (MQ=255)

                                                                                       tATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAg                                                            <  2:1390416/150‑1 (MQ=255)

                                                                                        aTGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGAc                                                          <  4:213436/151‑1 (MQ=255)

                                                                                           cATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGAt                                                       <  3:947613/151‑1 (MQ=255)

                                                                                                tctctcAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATttatt                                                  >  3:1191473/1‑151 (MQ=255)

                                                                                                  tctcAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATttatta                                                 >  2:159491/1‑150 (MQ=255)

                                                                                                    tcAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTaa                                                >  4:285773/1‑149 (MQ=255)

                                                                                                    tcAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTaa                                                >  3:210089/1‑149 (MQ=255)

                                                                                                     cAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAgg                                             >  2:1356875/1‑151 (MQ=255)

                                                                                                     cAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAgg                                             >  3:333968/1‑151 (MQ=255)

                                                                                                     cAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAg                                              <  3:1226089/150‑1 (MQ=255)

                                                                                                       gTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGtt                                           >  3:489619/1‑151 (MQ=255)

                                                                                                           aacaaTTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAAt                                       >  2:646072/1‑151 (MQ=255)

                                                                                                            acaaTTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATg                                      >  2:52614/1‑151 (MQ=255)

                                                                                                             caaTTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATgg                                     <  2:1247655/151‑1 (MQ=255)

                                                                                                               aTTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCt                                   >  2:369611/1‑151 (MQ=255)

                                                                                                                 tGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTAt                                 >  2:897774/1‑151 (MQ=255)

                                                                                                                 tGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTAt                                 >  2:326027/1‑151 (MQ=255)

                                                                                                                  ggATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTAt                                 <  2:325468/150‑1 (MQ=255)

                                                                                                                  ggATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATg                                >  2:900954/1‑151 (MQ=255)

                                                                                                                    attattAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGgc                              <  2:34220/151‑1 (MQ=255)

                                                                                                                     ttattaATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGgcc                             >  2:807376/1‑151 (MQ=255)

                                                                                                                     ttattaATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGgcc                             >  3:506593/1‑151 (MQ=255)

                                                                                                                      tattaATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGgccg                            >  2:417060/1‑151 (MQ=255)

                                                                                                                       attaATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGgccgc                           >  2:1389979/1‑151 (MQ=255)

                                                                                                                        ttaATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGgccgcc                          >  3:327041/1‑151 (MQ=255)

                                                                                                                           ataaataTTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCgtg                       <  3:1373140/151‑1 (MQ=255)

                                                                                                                            taaataTTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCgtgt                      >  2:1283004/1‑151 (MQ=255)

                                                                                                                               atatTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCg                   >  1:238419/1‑151 (MQ=255)

                                                                                                                               atatTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCg                   <  4:244603/151‑1 (MQ=255)

                                                                                                                                tatTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCgg                  >  1:453271/1‑151 (MQ=255)

                                                                                                                                 atTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCgg                  >  3:55027/1‑150 (MQ=255)

                                                                                                                                 atTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGt                 >  3:391446/1‑151 (MQ=255)

                                                                                                                                 atTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGt                 >  2:1191690/1‑151 (MQ=255)

                                                                                                                                   tGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTa                <  3:1117518/150‑1 (MQ=255)

                                                                                                                                   tGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTAc               >  3:402939/1‑151 (MQ=255)

                                                                                                                                        agagTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCa             <  4:256286/148‑1 (MQ=255)

                                                                                                                                        agagTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAg            <  3:840822/149‑1 (MQ=255)

                                                                                                                                        agagTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGc           <  2:292228/150‑1 (MQ=255)

                                                                                                                                        agagTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCg          <  3:1309671/151‑1 (MQ=255)

                                                                                                                                        agagTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCg          >  2:1215079/1‑151 (MQ=255)

                                                                                                                                           gTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCa       <  2:641564/151‑1 (MQ=255)

                                                                                                                                            tGAGCGGCCATAAATAAGCCCTTTCTTGCCGCTGAAAACGCCCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCa       <  3:1253242/150‑1 (MQ=255)

                                                                                                                                              aGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTg    <  4:337558/151‑1 (MQ=255)

                                                                                                                                              aGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGCGTGCGGTACCAGCGGCAtt     >  3:50598/1‑150 (MQ=255)

                                                                                                                                               gCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTGc   >  3:431245/1‑151 (MQ=255)

                                                                                                                                                cGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTGCt  <  3:1191747/151‑1 (MQ=255)

AAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTGCT  >  NC_000913/1496630‑1496924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`