Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,967,082 T→C K119R (AAA→AGA)  cheY ← chemotaxis regulator transmitting signal to flagellar motor component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,967,0820TC100.0% 445.8 / NA 111K119R (AAA→AGA) cheYchemotaxis regulator transmitting signal to flagellar motor component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (59/52);  total (59/52)

Only 100 of 112 total aligned reads displayed.

AGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTT  >  NC_000913/1966934‑1967229
                                                                                                                                                    |                                                                                                                                                   
aGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGtctt                                                                                                                                                   <  4:90903/151‑1 (MQ=255)
   tCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTtcc                                                                                                                                                >  2:767201/1‑151 (MQ=255)
        cAAACTGTCGCGCAGCATACGCGTCGGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTtcctcc                                                                                                                                             >  2:610690/1‑149 (MQ=255)
          aaCTGTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCg                                                                                                                                         <  3:422602/151‑1 (MQ=255)
          aaCTGTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCg                                                                                                                                         >  3:289484/1‑151 (MQ=255)
          aaCTGTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCg                                                                                                                                         >  3:612456/1‑151 (MQ=255)
           aCTGTCGCGCAGCATACGCGTTAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGt                                                                                                                                        >  2:1055723/1‑151 (MQ=255)
           aCTGTCGAGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGt                                                                                                                                        >  4:206202/1‑151 (MQ=255)
             tGTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTcg                                                                                                                                      >  2:650158/1‑151 (MQ=255)
              gTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTcg                                                                                                                                      <  2:427237/150‑1 (MQ=255)
              gTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTc                                                                                                                                       <  3:707184/149‑1 (MQ=255)
                cgcgCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCgcgg                                                                                                                                   <  4:223884/151‑1 (MQ=255)
                 gcgcAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCgcggc                                                                                                                                  >  3:555288/1‑151 (MQ=255)
                  cgcAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCgcggcg                                                                                                                                 <  2:448277/151‑1 (MQ=255)
                      gcaTACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTa                                                                                                                              >  3:1127635/1‑150 (MQ=255)
                        aTACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAAt                                                                                                                           >  2:583116/1‑151 (MQ=255)
                          aCGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATg                                                                                                                          <  3:870338/150‑1 (MQ=255)
                            gcgTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCTTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGACt                                                                                                                       <  3:675571/151‑1 (MQ=255)
                            gcgTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCt                                                                                                                       >  2:1131966/1‑151 (MQ=255)
                                cAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTcac                                                                                                                   >  3:770610/1‑151 (MQ=255)
                                  ggCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTcacca                                                                                                                 <  2:821923/151‑1 (MQ=255)
                                  ggCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTcacc                                                                                                                  >  2:1297606/1‑150 (MQ=255)
                                   gCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTcaccac                                                                                                                <  1:74842/151‑1 (MQ=255)
                                    cTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTcaccac                                                                                                                >  2:299362/1‑150 (MQ=255)
                                     tGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACAt                                                                                                              >  4:28094/1‑151 (MQ=255)
                                      gCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATa                                                                                                             <  2:345245/151‑1 (MQ=255)
                                         gATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAAcc                                                                                                          >  4:295073/1‑151 (MQ=255)
                                          aTGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCa                                                                                                         <  2:785973/151‑1 (MQ=255)
                                           tGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGcc                                                                                                          <  2:243268/149‑1 (MQ=255)
                                           tGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGcc                                                                                                          >  3:243293/1‑149 (MQ=255)
                                            gcgcgcAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCAc                                                                                                        <  2:701661/150‑1 (MQ=255)
                                                 cAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGccc                                                                                                  >  3:341198/1‑151 (MQ=255)
                                                    tGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGccccc                                                                                                >  1:115184/1‑150 (MQ=255)
                                                    tGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGccccc                                                                                                >  3:827036/1‑150 (MQ=255)
                                                     gATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGccccc                                                                                                >  3:1279037/1‑149 (MQ=255)
                                                     gATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGc                                                                                              <  2:555257/151‑1 (MQ=255)
                                                        atCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCtt                                                                                            >  2:862860/1‑150 (MQ=255)
                                                        atCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCtt                                                                                            >  3:356073/1‑150 (MQ=255)
                                                         tCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTgc                                                                                          >  4:102893/1‑151 (MQ=255)
                                                             cAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGcgcc                                                                                       >  3:1273553/1‑150 (MQ=255)
                                                               gCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGTATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGcgccgc                                                                                     >  2:330242/1‑150 (MQ=255)
                                                               gCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCACCCCCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCGCCAGCGTCGCGGCGGTAAATGGCTTCTCCACATTGCCACTGGCCCCCGCTTGcgccgc                                                                                     <  3:1279875/150‑1 (MQ=255)
                                                                   aaTGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCgcagca                                                                                 >  2:1050798/1‑150 (MQ=255)
                                                                    aTGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAat                                                                               >  3:86121/1‑151 (MQ=255)
                                                                    aTGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAat                                                                               >  1:374343/1‑151 (MQ=255)
                                                                      gCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAatg                                                                              <  2:52503/150‑1 (MQ=255)
                                                                           tCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGtt                                                                         >  2:1117772/1‑150 (MQ=255)
                                                                            cagcagGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTtct                                                                       <  2:495137/151‑1 (MQ=255)
                                                                              gcagGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTctct                                                                     <  4:321672/151‑1 (MQ=255)
                                                                               cagGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCCGCAATGATGTTCtctt                                                                    >  3:1011754/1‑151 (MQ=255)
                                                                               cagGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCtctt                                                                    >  3:1150662/1‑151 (MQ=255)
                                                                                 ggTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCtctttc                                                                  <  3:975219/151‑1 (MQ=255)
                                                                                  gTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCtctttct                                                                 <  2:799779/151‑1 (MQ=255)
                                                                                     tgattgatGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCg                                                              >  3:689912/1‑151 (MQ=255)
                                                                                     tgattgatGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCg                                                              >  2:528280/1‑151 (MQ=255)
                                                                                      gattgatGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCg                                                              <  3:62035/150‑1 (MQ=255)
                                                                                       attgatGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCt                                                            <  2:1127580/151‑1 (MQ=255)
                                                                                       attgatGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCt                                                            >  2:416065/1‑151 (MQ=255)
                                                                                          gatGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCt                                                         <  4:366648/151‑1 (MQ=255)
                                                                                             ggTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGc                                                       <  4:475028/150‑1 (MQ=255)
                                                                                             ggTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGc                                                       <  2:1073995/150‑1 (MQ=255)
                                                                                                 gCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCa                                                  <  3:1244570/151‑1 (MQ=255)
                                                                                                 gCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCa                                                  <  4:199659/151‑1 (MQ=255)
                                                                                                 gCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCa                                                  <  4:134368/151‑1 (MQ=255)
                                                                                                  catcatAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCAc                                                 <  1:186722/151‑1 (MQ=255)
                                                                                                     catAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCAt                                              <  1:310894/151‑1 (MQ=255)
                                                                                                      atAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCAtt                                             <  1:72933/151‑1 (MQ=255)
                                                                                                       tAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCAtt                                             >  4:135306/1‑150 (MQ=255)
                                                                                                         gTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAac                                          <  2:199400/151‑1 (MQ=255)
                                                                                                             cATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTg                                      >  3:1329819/1‑151 (MQ=255)
                                                                                                              aTCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTgg                                     >  3:454598/1‑151 (MQ=255)
                                                                                                              aTCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTgg                                     >  3:65607/1‑151 (MQ=255)
                                                                                                               tCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGc                                    >  2:1055344/1‑151 (MQ=255)
                                                                                                               tCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGc                                    >  3:439206/1‑151 (MQ=255)
                                                                                                               tCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGc                                    >  1:31489/1‑151 (MQ=255)
                                                                                                                ccTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGc                                    >  1:245983/1‑150 (MQ=255)
                                                                                                                ccTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCa                                   <  2:628825/151‑1 (MQ=255)
                                                                                                                ccTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCa                                   <  2:356042/151‑1 (MQ=255)
                                                                                                                 cTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCa                                   >  2:401293/1‑150 (MQ=255)
                                                                                                                   cacaTGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCTCCATTAACACTGGCaa                                  <  4:18290/149‑1 (MQ=255)
                                                                                                                    acaTGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGc                               >  1:186802/1‑151 (MQ=255)
                                                                                                                       tGCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCg                             >  3:1170280/1‑150 (MQ=255)
                                                                                                                        gCCCAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGa                            <  2:341169/150‑1 (MQ=255)
                                                                                                                         cccAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACa                          <  2:31542/151‑1 (MQ=255)
                                                                                                                         cccAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACa                          <  2:1234370/151‑1 (MQ=255)
                                                                                                                          ccAGTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCCTCGCTTCTGCACTCACCATTAACACTGGCCATGCCGGCAt                         >  3:269639/1‑151 (MQ=255)
                                                                                                                             gTTTCTCAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATcgc                      >  3:465820/1‑151 (MQ=255)
                                                                                                                                tctcAAAGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCgcgc                    <  2:612429/150‑1 (MQ=255)
                                                                                                                                     aaGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCg              <  3:157743/151‑1 (MQ=255)
                                                                                                                                      aGATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGc             <  3:234013/151‑1 (MQ=255)
                                                                                                                                       gATTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGc             >  2:1307438/1‑150 (MQ=255)
                                                                                                                                        aTTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCAc           >  3:966836/1‑151 (MQ=255)
                                                                                                                                        aTTTTGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCAc           <  1:363503/151‑1 (MQ=255)
                                                                                                                                          tttGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACg          <  3:655923/150‑1 (MQ=255)
                                                                                                                                          tttGTTGAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACg          <  3:1281545/150‑1 (MQ=255)
                                                                                                                                              ttgAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTg     >  4:334200/1‑151 (MQ=255)
                                                                                                                                                gAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGtt   <  2:770575/151‑1 (MQ=255)
                                                                                                                                                gAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGtt   >  2:255351/1‑151 (MQ=255)
                                                                                                                                                gAGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGt    <  3:632/150‑1 (MQ=255)
                                                                                                                                                 aGTCTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGttt  <  1:116370/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGTTCCCGCAAACTGTCGCGCAGCATACGCGTCAGGCTGCCGATGCGCGCAATGATATCGCCAGCTGAATGCTCGTCAGCAGGTTTGATTGATGGTTGCATCATAGTCGCATCCTCACATGCCCAGTTTCTCAAAGATTTTGTTGAGTTTTTCCTCCAGCGTCGCGGCGGTAAATGGCTTCACCACATAGCCACTGGCCCCCGCTTGCGCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTT  >  NC_000913/1966934‑1967229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑