BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,140,009	T→C	D535G (GAC→GGC)	asmA ←	suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,140,009	0	T	C	100.0%	415.9 / NA	114	D535G (GAC→GGC)	asmA	suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (51/63); total (51/63)

Only 100 of 114 total aligned reads displayed.

TTGCGCAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAA  >  NC_000913/2139862‑2140154

ttGCGCAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCg                                                                                                                                                 <  2:776084/150‑1 (MQ=255)

ttGCGCAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCg                                                                                                                                                 >  3:771489/1‑150 (MQ=255)

ttGCGCAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCg                                                                                                                                                 <  3:416045/150‑1 (MQ=255)

    gcAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGt                                                                                                                                            <  3:175732/151‑1 (MQ=255)

     cAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGtt                                                                                                                                           <  2:368503/151‑1 (MQ=255)

     cAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGtt                                                                                                                                           <  4:404/151‑1 (MQ=255)

     cAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGtt                                                                                                                                           <  2:1188190/151‑1 (MQ=255)

      aGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGttt                                                                                                                                          <  3:109585/151‑1 (MQ=255)

      aGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGttt                                                                                                                                          <  2:1260415/151‑1 (MQ=255)

      aGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGttt                                                                                                                                          <  3:548220/151‑1 (MQ=255)

          aCTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATc                                                                                                                                      <  2:743897/151‑1 (MQ=255)

             gATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCt                                                                                                                                     <  3:674473/149‑1 (MQ=255)

             gATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACACGAATATCAAACTGGGCGCCGCAGGTTTGATCTg                                                                                                                                    >  3:1191420/1‑150 (MQ=255)

                ccACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCa                                                                                                                                 <  3:147525/150‑1 (MQ=255)

                ccACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCa                                                                                                                                 <  3:162546/150‑1 (MQ=255)

                 cACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCATGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGTTGCCGCAGGTTTGATCTGCCAGa                                                                                                                               <  1:102354/151‑1 (MQ=255)

                 cACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGa                                                                                                                               <  3:605632/151‑1 (MQ=255)

                   cTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGAtt                                                                                                                             >  4:157476/1‑151 (MQ=255)

                     tGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGAtt                                                                                                                             <  2:1297072/149‑1 (MQ=255)

                     tGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTc                                                                                                                            >  2:1010087/1‑150 (MQ=255)

                        aGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAAc                                                                                                                         >  2:901208/1‑150 (MQ=255)

                        aGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAAc                                                                                                                         >  1:320211/1‑150 (MQ=255)

                         ggCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACAt                                                                                                                       >  2:848759/1‑151 (MQ=255)

                           cTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGc                                                                                                                     >  3:76490/1‑151 (MQ=255)

                             gTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGcc                                                                                                                    <  2:321695/150‑1 (MQ=255)

                             gTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCt                                                                                                                   <  3:458826/151‑1 (MQ=255)

                              tAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCtt                                                                                                                  >  2:1061829/1‑151 (MQ=255)

                               aaTTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCACCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGCTTGATCTGCCAGATTCAACATGCCTTc                                                                                                                 >  3:666535/1‑151 (MQ=255)

                                 ttGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTccc                                                                                                               >  4:382429/1‑151 (MQ=255)

                                   gAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCt                                                                                                             <  3:671722/151‑1 (MQ=255)

                                   gAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCt                                                                                                             <  3:611015/151‑1 (MQ=255)

                                    aGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTg                                                                                                            <  2:1233341/151‑1 (MQ=255)

                                     gTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGt                                                                                                           <  4:372004/151‑1 (MQ=255)

                                       tgctgcCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTc                                                                                                          <  1:109049/150‑1 (MQ=255)

                                        gctgcCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTc                                                                                                          >  1:429731/1‑149 (MQ=255)

                                           gcCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAgcgc                                                                                                     >  3:457955/1‑151 (MQ=255)

                                            ccAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGcc                                                                                                    >  2:1243414/1‑151 (MQ=255)

                                             cAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGcc                                                                                                    >  1:278122/1‑150 (MQ=255)

                                              aGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGcc                                                                                                    >  4:450550/1‑149 (MQ=255)

                                              aGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATAAGTTTGCTTTCCCCGTTCCAGCCACCCACGACACGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGcc                                                                                                    >  4:263325/1‑149 (MQ=255)

                                                    cATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAgg                                                                                            <  2:622598/151‑1 (MQ=255)

                                                     aTAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGc                                                                                           >  2:1369552/1‑151 (MQ=255)

                                                     aTAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGc                                                                                           <  2:824436/151‑1 (MQ=255)

                                                       aaaCCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCg                                                                                          >  4:451764/1‑150 (MQ=255)

                                                       aaaCCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCg                                                                                          <  3:470214/150‑1 (MQ=255)

                                                       aaaCCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCg                                                                                          <  1:370390/150‑1 (MQ=255)

                                                        aaCCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGAt                                                                                        >  2:719274/1‑151 (MQ=255)

                                                         aCCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGAtt                                                                                       <  1:450554/151‑1 (MQ=255)

                                                          cccGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTg                                                                                      <  3:442194/151‑1 (MQ=255)

                                                               aGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCtt                                                                                 <  1:305128/151‑1 (MQ=255)

                                                                gCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTg                                                                                <  3:38333/151‑1 (MQ=255)

                                                                 cGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGc                                                                               >  3:274228/1‑151 (MQ=255)

                                                                   gTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCAt                                                                             >  1:241259/1‑151 (MQ=255)

                                                                        ggCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTc                                                                          <  3:1117479/149‑1 (MQ=255)

                                                                          cGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGttt                                                                      <  3:529267/151‑1 (MQ=255)

                                                                          cGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGttt                                                                      >  3:1255366/1‑151 (MQ=255)

                                                                            tttctttcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGGGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGttt                                                                      >  3:1307865/1‑149 (MQ=255)

                                                                             ttctttcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAAc                                                                   >  1:398222/1‑151 (MQ=255)

                                                                             ttctttcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAAc                                                                   >  1:215949/1‑151 (MQ=255)

                                                                              tctttcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGTATGTCGTTTAAc                                                                   <  2:76480/150‑1 (MQ=255)

                                                                              tctttcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAAc                                                                   <  2:892761/150‑1 (MQ=255)

                                                                                 ttcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTc                                                                >  3:225943/1‑150 (MQ=255)

                                                                                 ttcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTc                                                                >  3:95447/1‑150 (MQ=255)

                                                                                 ttcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTc                                                                <  2:1314523/150‑1 (MQ=255)

                                                                                   cAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCacg                                                             >  2:492188/1‑151 (MQ=255)

                                                                                   cAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCacg                                                             <  2:388566/151‑1 (MQ=255)

                                                                                      gAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCacgacg                                                          >  1:72001/1‑151 (MQ=255)

                                                                                       aaaTCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGc                                                         <  2:1272557/151‑1 (MQ=255)

                                                                                        aatcaatcAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGACTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGcc                                                        <  2:1381522/151‑1 (MQ=255)

                                                                                         atcaatcaGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGcc                                                        >  2:477003/1‑150 (MQ=255)

                                                                                         atcaatcaGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCa                                                       <  2:723263/151‑1 (MQ=255)

                                                                                         atcaatcaGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCa                                                       <  2:771454/151‑1 (MQ=255)

                                                                                                aGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTc                                                 >  2:1230715/1‑150 (MQ=255)

                                                                                                   ttGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAAc                                              <  1:160611/150‑1 (MQ=255)

                                                                                                   ttGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAAc                                              <  2:257713/150‑1 (MQ=255)

                                                                                                    tGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGt                                            >  2:1084345/1‑151 (MQ=255)

                                                                                                     gCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTc                                           >  2:545030/1‑151 (MQ=255)

                                                                                                       tttCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCa                                          >  2:1173949/1‑150 (MQ=255)

                                                                                                       tttCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCa                                          >  2:1001309/1‑150 (MQ=255)

                                                                                                        ttCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCaa                                         >  4:406089/1‑150 (MQ=255)

                                                                                                          ccccGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAAt                                       <  2:456370/150‑1 (MQ=255)

                                                                                                              gTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCggtg                                  <  2:1296748/151‑1 (MQ=255)

                                                                                                              gTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCggtg                                  >  3:893776/1‑151 (MQ=255)

                                                                                                              gTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCggtg                                  <  3:38724/151‑1 (MQ=255)

                                                                                                              gTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCggtg                                  >  2:673837/1‑151 (MQ=255)

                                                                                                                 ccAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCggtggt                                >  3:1387851/1‑150 (MQ=255)

                                                                                                                   aGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTaaa                             <  3:1066612/151‑1 (MQ=255)

                                                                                                                   aGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTaaa                             <  2:274201/151‑1 (MQ=255)

                                                                                                                      cacccacGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCg                          >  2:652849/1‑151 (MQ=255)

                                                                                                                       acccacGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCgg                         >  3:729870/1‑151 (MQ=255)

                                                                                                                            cGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAg                    <  2:797648/151‑1 (MQ=255)

                                                                                                                             gACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGc                   >  2:570717/1‑151 (MQ=255)

                                                                                                                               cccGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCcg                 <  3:835977/151‑1 (MQ=255)

                                                                                                                                 cGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCcgcg               <  3:1061874/151‑1 (MQ=255)

                                                                                                                                 cGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCcgcg               >  2:337935/1‑151 (MQ=255)

                                                                                                                                        cAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGtt        >  2:436682/1‑151 (MQ=255)

                                                                                                                                        cAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGtt        <  3:1016042/151‑1 (MQ=255)

                                                                                                                                          aaCTGGGGGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTAt      >  3:1182009/1‑151 (MQ=255)

                                                                                                                                              gggTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCaaa  <  2:729839/151‑1 (MQ=255)

                                                                                                                                              gggTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCa    <  2:95433/149‑1 (MQ=255)

TTGCGCAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAA  >  NC_000913/2139862‑2140154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`