Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,223,301 T→C F373L (TTC→CTC)  dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,223,3010TC99.1% 420.7 / ‑4.1 112F373L (TTC→CTC) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (53/57);  total (54/58)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01

Only 100 of 112 total aligned reads displayed.

CACCTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGTTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTC  >  NC_000913/2223156‑2223447
                                                                                                                                                 |                                                                                                                                                  
cacCTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTcccc                                                                                                                                               >  3:195322/1‑151 (MQ=255)
 acCTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTcccc                                                                                                                                               >  3:804344/1‑150 (MQ=255)
       cTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCAtt                                                                                                                                        >  1:226384/1‑151 (MQ=255)
        tgatgaTTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCAttt                                                                                                                                       >  3:311487/1‑151 (MQ=255)
         gatgatTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCAttt                                                                                                                                       <  2:303866/150‑1 (MQ=255)
         gatgatTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCAttt                                                                                                                                       >  2:1283625/1‑150 (MQ=255)
           tgattgatAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTAcc                                                                                                                                    >  4:381332/1‑151 (MQ=255)
            gattgatAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTAcc                                                                                                                                    <  2:928181/150‑1 (MQ=255)
               tgatAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTAccgccg                                                                                                                                >  3:1076236/1‑151 (MQ=255)
               tgatAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTAccgccg                                                                                                                                >  2:709639/1‑151 (MQ=255)
                gatAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTAccgccg                                                                                                                                <  2:1273982/150‑1 (MQ=255)
                  tAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCgcgc                                                                                                                             >  3:544025/1‑151 (MQ=255)
                   aaGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCgcgc                                                                                                                             <  2:299039/150‑1 (MQ=255)
                    aGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCAt                                                                                                                           >  2:872331/1‑151 (MQ=255)
                    aGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCAt                                                                                                                           >  1:193860/1‑151 (MQ=255)
                      ttAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATg                                                                                                                          >  2:629762/1‑150 (MQ=255)
                      ttAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATg                                                                                                                          >  2:366924/1‑150 (MQ=255)
                        aGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGaa                                                                                                                        >  3:515358/1‑150 (MQ=255)
                         ggCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGaaaa                                                                                                                      <  3:1060984/151‑1 (MQ=255)
                         ggCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGaaaa                                                                                                                      <  2:980613/151‑1 (MQ=255)
                            aCCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACt                                                                                                                   >  3:18260/1‑151 (MQ=255)
                             ccGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTg                                                                                                                  <  2:170362/151‑1 (MQ=255)
                             ccGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTg                                                                                                                  <  2:293025/151‑1 (MQ=255)
                                aCAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGgcg                                                                                                               <  3:209147/151‑1 (MQ=255)
                                 cAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGgcgc                                                                                                              <  2:155082/151‑1 (MQ=255)
                                   aGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGcgcg                                                                                                             >  3:487257/1‑150 (MQ=255)
                                   aGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGa                                                                                                            <  3:53627/151‑1 (MQ=255)
                                      tGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATa                                                                                                          >  2:706101/1‑150 (MQ=255)
                                       gCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATaaa                                                                                                        <  4:344997/151‑1 (MQ=255)
                                        ccGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATaaa                                                                                                        <  3:262658/150‑1 (MQ=255)
                                        ccGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATaaa                                                                                                        >  2:262629/1‑150 (MQ=255)
                                        ccGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAAt                                                                                                       <  1:246242/151‑1 (MQ=255)
                                        ccGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAAt                                                                                                       <  3:652579/151‑1 (MQ=255)
                                         cGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAAt                                                                                                       >  3:1331413/1‑150 (MQ=255)
                                          gTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATAc                                                                                                     <  4:247794/151‑1 (MQ=255)
                                           ttcttcTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACg                                                                                                    >  1:313755/1‑151 (MQ=255)
                                            tcttctTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACg                                                                                                    <  3:1074865/150‑1 (MQ=255)
                                             cttcttTAATCTCAAGGGATGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACg                                                                                                    >  4:316441/1‑149 (MQ=255)
                                               tcttTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAg                                                                                                  <  2:1130972/149‑1 (MQ=255)
                                               tcttTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAg                                                                                                  <  2:769628/149‑1 (MQ=255)
                                                cttTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGcat                                                                                               >  2:418981/1‑151 (MQ=255)
                                                  ttAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGcatc                                                                                              >  1:317731/1‑150 (MQ=255)
                                                   tAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGcatcat                                                                                            >  3:706637/1‑151 (MQ=255)
                                                    aaTCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGcatcat                                                                                            <  1:47721/150‑1 (MQ=255)
                                                         cAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATct                                                                                          <  1:280744/147‑1 (MQ=255)
                                                          aaGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGt                                                                                     >  3:964199/1‑151 (MQ=255)
                                                          aaGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGt                                                                                     >  3:845184/1‑151 (MQ=255)
                                                           aGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGtt                                                                                    <  2:521770/151‑1 (MQ=255)
                                                           aGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGtt                                                                                    >  2:51718/1‑151 (MQ=255)
                                                           aGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGtt                                                                                    >  4:252145/1‑151 (MQ=255)
                                                             ggACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTaa                                                                                  <  3:1052064/151‑1 (MQ=255)
                                                             ggACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTaa                                                                                  <  2:1041712/151‑1 (MQ=255)
                                                              gACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTaaa                                                                                 <  3:1360520/151‑1 (MQ=255)
                                                              gACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTaaa                                                                                 <  2:557373/151‑1 (MQ=255)
                                                                  cACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATg                                                                             <  2:878611/151‑1 (MQ=255)
                                                                     cgatgcgatgCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcg                                                                          >  1:7300/1‑151 (MQ=255)
                                                                      gatgcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGATCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcgg                                                                         <  3:1167666/151‑1 (MQ=255)
                                                                       atgcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcggg                                                                        <  2:921689/151‑1 (MQ=255)
                                                                         gcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcgggcg                                                                      >  2:488654/1‑151 (MQ=255)
                                                                         gcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcgggc                                                                       <  2:1176947/150‑1 (MQ=255)
                                                                         gcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcgggc                                                                       <  2:311458/150‑1 (MQ=255)
                                                                              gcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGc                                                                 <  2:17768/151‑1 (MQ=255)
                                                                                               tcttcCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAAt                                                 <  2:804309/150‑1 (MQ=255)
                                                                                               tcttcCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAAt                                                 <  2:747896/150‑1 (MQ=255)
                                                                                                 ttcCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGt                                              >  2:932027/1‑151 (MQ=255)
                                                                                                  tccgtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAAtag                                               <  2:1076190/149‑3 (MQ=255)
                                                                                                  tccgtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCg                                               <  3:419011/149‑1 (MQ=255)
                                                                                                   ccgtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTgg                                            <  2:18254/151‑1 (MQ=255)
                                                                                                   ccgtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTg                                             <  2:764969/150‑1 (MQ=255)
                                                                                                     gtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCt                                          <  3:1092654/151‑1 (MQ=255)
                                                                                                     gtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCt                                          >  1:36097/1‑151 (MQ=255)
                                                                                                       ccgCATTTTACTGACTGTGCGATGCAAAAATTCGGTCACCTGTTACCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCACAGGCGAACCCCAAtacggggggg                                        >  3:595592/1‑142 (MQ=255)
                                                                                                       ccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtgg                                        <  3:48804/151‑1 (MQ=255)
                                                                                                        cgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggt                                       <  4:286898/151‑1 (MQ=255)
                                                                                                          cATTTTACTGACCGTGCTATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggtgg                                     <  2:195293/151‑1 (MQ=255)
                                                                                                           aTTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggtgg                                     >  1:274618/1‑150 (MQ=255)
                                                                                                              ttACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGgtt                                  >  4:403004/1‑150 (MQ=255)
                                                                                                              ttACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGatt                                  >  3:971278/1‑150 (MQ=255)
                                                                                                                   gACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTAttt                              <  1:285514/149‑1 (MQ=255)
                                                                                                                    aCCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTAttt                              >  3:1146968/1‑148 (MQ=255)
                                                                                                                     ccGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCaaa                          <  3:1283691/151‑1 (MQ=255)
                                                                                                                      cGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAAc                         <  4:31325/151‑1 (MQ=255)
                                                                                                                         gCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAg                       <  2:568699/150‑1 (MQ=255)
                                                                                                                         gCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAg                       <  2:225248/150‑1 (MQ=255)
                                                                                                                          cGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGc                     >  2:1329896/1‑151 (MQ=255)
                                                                                                                           gATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGcc                    <  2:862377/151‑1 (MQ=255)
                                                                                                                            aTGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCg                   <  3:1035873/151‑1 (MQ=255)
                                                                                                                                aaaaaTTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAgg               <  2:1004514/151‑1 (MQ=255)
                                                                                                                                aaaaaTTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAgg               <  3:51729/151‑1 (MQ=255)
                                                                                                                                  aaaTTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCg             >  2:625782/1‑151 (MQ=255)
                                                                                                                                   aaTTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGa            <  2:543991/151‑1 (MQ=255)
                                                                                                                                      tCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGAtt          <  2:865555/150‑1 (MQ=255)
                                                                                                                                      tCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTATTTTAAACAGGGCCAAGGCGAttt         >  3:1010653/1‑151 (MQ=255)
                                                                                                                                        ggTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCt       >  2:1082058/1‑151 (MQ=255)
                                                                                                                                         gTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCtt      <  2:487223/151‑1 (MQ=255)
                                                                                                                                         gTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGGTTATTTTAAAAAGGCCGGAGGCGATTTCtt      >  3:826893/1‑151 (MQ=255)
                                                                                                                                          tCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCttt     >  1:97609/1‑151 (MQ=255)
                                                                                                                                           cACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCttt     >  2:146444/1‑150 (MQ=255)
                                                                                                                                            aCCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGt   >  2:660826/1‑151 (MQ=255)
                                                                                                                                              cTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAAGCGATTTCTTTGTc  >  2:910708/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CACCTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGTTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGTC  >  NC_000913/2223156‑2223447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑