BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,487,085	A→G	L904L (TTA→TTG)	evgS →	hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,487,085	0	A	G	100.0%	526.3 / NA	134	L904L (TTA→TTG)	evgS	hybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (58/76); total (58/76)

Only 100 of 135 total aligned reads displayed.

TCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTAATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGCAAAAGCAGAACAACCCAT  >  NC_000913/2486941‑2487231

tCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTgatgat                                                                                                                                               <  2:606130/150‑1 (MQ=255)

 cacaTTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATc                                                                                                                                              <  2:245085/150‑1 (MQ=255)

 cacaTTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCt                                                                                                                                             <  2:498320/151‑1 (MQ=255)

   caTTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGc                                                                                                                                           >  3:785598/1‑151 (MQ=255)

    aTTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGc                                                                                                                                           >  2:267229/1‑150 (MQ=255)

    aTTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCa                                                                                                                                          <  2:43180/151‑1 (MQ=255)

      tgatgaCAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCaa                                                                                                                                         <  1:139803/150‑1 (MQ=255)

       gatgaCAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAg                                                                                                                                       <  2:1103593/151‑1 (MQ=255)

         tgaCAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGACGAACAACAACGACTGATTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGCTCTGGTTTAGGCTTGATGATCTGCAAAGa                                                                                                                                      >  4:31280/1‑150 (MQ=255)

         tgaCAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGa                                                                                                                                      >  1:321962/1‑150 (MQ=255)

           aCAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGaatt                                                                                                                                   >  2:1331791/1‑151 (MQ=255)

              aCCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGaattaat                                                                                                                                <  3:356379/151‑1 (MQ=255)

              aCCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGaattaat                                                                                                                                <  3:25543/151‑1 (MQ=255)

                cACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGaattaatt                                                                                                                               >  3:678389/1‑150 (MQ=255)

                  cGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTaaa                                                                                                                            <  3:910478/151‑1 (MQ=255)

                   gCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTaaa                                                                                                                            >  4:301806/1‑150 (MQ=255)

                    cTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTaaaa                                                                                                                           <  2:503201/150‑1 (MQ=255)

                     tGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAat                                                                                                                         >  2:766333/1‑151 (MQ=255)

                      gTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAata                                                                                                                        <  4:160441/151‑1 (MQ=255)

                        tATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAatat                                                                                                                       <  2:540905/150‑1 (MQ=255)

                        tATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAatat                                                                                                                       <  2:97730/150‑1 (MQ=255)

                          tCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGc                                                                                                                     >  4:225005/1‑150 (MQ=255)

                           cAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAg                                                                                                                   <  1:380451/151‑1 (MQ=255)

                           cAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGACAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAg                                                                                                                   <  3:892510/151‑1 (MQ=255)

                            aaaaTGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAgg                                                                                                                  <  2:890703/151‑1 (MQ=255)

                                 gACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGa                                                                                                              <  3:820720/150‑1 (MQ=255)

                                 gACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGAt                                                                                                             <  3:875806/151‑1 (MQ=255)

                                  aCGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGAtt                                                                                                            >  4:221867/1‑151 (MQ=255)

                                   cGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGAttt                                                                                                           >  2:1399223/1‑151 (MQ=255)

                                   cGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGAttt                                                                                                           <  2:1300022/151‑1 (MQ=255)

                                   cGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGAttt                                                                                                           <  1:457624/151‑1 (MQ=255)

                                     aTTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGt                                                                                                         >  4:157823/1‑151 (MQ=255)

                                     aTTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGt                                                                                                         >  4:287579/1‑151 (MQ=255)

                                      ttATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTc                                                                                                        >  3:828639/1‑151 (MQ=255)

                                       tATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTc                                                                                                        >  3:686676/1‑150 (MQ=255)

                                        aTGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCa                                                                                                       <  2:1148212/150‑1 (MQ=255)

                                         tGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCAtt                                                                                                     >  4:86254/1‑151 (MQ=255)

                                          ggATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCAtt                                                                                                     >  1:112582/1‑150 (MQ=255)

                                            aTTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCAtt                                                                                                     >  2:792109/1‑148 (MQ=255)

                                            aTTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGa                                                                                                  <  2:1212640/151‑1 (MQ=255)

                                               cTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGaa                                                                                                 >  3:608239/1‑149 (MQ=255)

                                                tGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGt                                                                                              >  2:640337/1‑151 (MQ=255)

                                                 ggAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGaaa                                                                                                <  1:237736/148‑1 (MQ=255)

                                                 ggAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGt                                                                                              <  1:383905/150‑1 (MQ=255)

                                                 ggAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTc                                                                                             >  2:1293934/1‑151 (MQ=255)

                                                  gAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTc                                                                                             >  3:336935/1‑150 (MQ=255)

                                                  gAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCa                                                                                            <  4:460858/151‑1 (MQ=255)

                                                     gTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAATCAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCAt                                                                                           <  2:77709/149‑1 (MQ=255)

                                                     gTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATcc                                                                                         <  3:1200349/151‑1 (MQ=255)

                                                       ggATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCa                                                                                        <  2:584890/150‑1 (MQ=255)

                                                           tATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCAt                                                                                   >  3:189383/1‑151 (MQ=255)

                                                              cGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAg                                                                                 <  3:1231173/150‑1 (MQ=255)

                                                                 aGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGaac                                                                             <  1:10424/151‑1 (MQ=255)

                                                                  ggAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGaaca                                                                            <  1:312480/151‑1 (MQ=255)

                                                                     agaaCAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGaacaac                                                                          >  3:554270/1‑150 (MQ=255)

                                                                       aacaacaacaacTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACAtt                                                                       <  3:513717/151‑1 (MQ=255)

                                                                        acaacaacaacTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAgg                                                                                      <  1:248352/135‑1 (MQ=255)

                                                                        acaacaacaacTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAgg                                                                                      >  4:248349/1‑135 (MQ=255)

                                                                        acaacaacaacTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACAttt                                                                      <  2:997950/151‑1 (MQ=255)

                                                                        acaacaacaacTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACAttt                                                                      <  3:766368/151‑1 (MQ=255)

                                                                         caacaacaacTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACAttt                                                                      >  3:326916/1‑150 (MQ=255)

                                                                         caacaacaacTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTa                                                                     <  1:98793/151‑1 (MQ=255)

                                                                         caacaacaacTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTa                                                                     <  1:461739/151‑1 (MQ=255)

                                                                         caacaacaacTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTa                                                                     <  4:287271/151‑1 (MQ=255)

                                                                            caacaacTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGa                                                                  <  3:1090513/151‑1 (MQ=255)

                                                                                      ttAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATccc                                                        >  3:1026770/1‑151 (MQ=255)

                                                                                       tAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGG‑ACAACATTTACGATCACAATCCCgg                                                      >  1:480615/1‑151 (MQ=255)

                                                                                         aaCGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGt                                                     >  3:1116735/1‑151 (MQ=255)

                                                                                         aaCGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGt                                                     <  3:754247/151‑1 (MQ=255)

                                                                                          aCGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTa                                                    <  1:225007/151‑1 (MQ=255)

                                                                                           cGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGt                                                     >  4:352268/1‑149 (MQ=255)

                                                                                           cGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAg                                                   <  2:803911/151‑1 (MQ=255)

                                                                                            gCTACAGCCAAACAAGGGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCg                                                       >  4:430094/1‑146 (MQ=255)

                                                                                             cTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTa                                                                     >  3:1248241/1‑131 (MQ=255)

                                                                                               aCAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAAt                                               <  1:284341/151‑1 (MQ=255)

                                                                                               aCAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAAt                                               >  3:753230/1‑151 (MQ=255)

                                                                                               aCAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAAt                                               <  2:678365/151‑1 (MQ=255)

                                                                                                  gCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAg                                            <  3:133197/151‑1 (MQ=255)

                                                                                                   ccAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGc                                           >  3:776978/1‑151 (MQ=255)

                                                                                                        cAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCa                                      <  4:432956/151‑1 (MQ=255)

                                                                                                          aGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCa                                      >  3:330711/1‑149 (MQ=255)

                                                                                                           gTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGt                                   >  3:264230/1‑151 (MQ=255)

                                                                                                            tGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTg                                  >  2:1344603/1‑151 (MQ=255)

                                                                                                                 gtcgtcAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGAc                             >  2:1097343/1‑151 (MQ=255)

                                                                                                                     tcAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTc                         >  4:72709/1‑151 (MQ=255)

                                                                                                                     tcAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTc                         >  3:268652/1‑151 (MQ=255)

                                                                                                                      cAGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTc                         <  3:1331864/150‑1 (MQ=255)

                                                                                                                       aGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCg                        >  2:394808/1‑150 (MQ=255)

                                                                                                                       aGCAAACAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCg                        >  2:1303276/1‑150 (MQ=255)

                                                                                                                          aaaCAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGc                    >  3:457176/1‑151 (MQ=255)

                                                                                                                          aaaCAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGc                    >  3:350855/1‑151 (MQ=255)

                                                                                                                          aaaCAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGc                    >  4:335812/1‑151 (MQ=255)

                                                                                                                           aaCAGGTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGc                    >  1:372339/1‑150 (MQ=255)

                                                                                                                                gTTCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGCAAAAGc              >  2:809272/1‑151 (MQ=255)

                                                                                                                                 ttCTGGTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGCAAAAGc              >  3:50196/1‑150 (MQ=255)

                                                                                                                                     ggTTTAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGCAAAAGCAg            <  3:267255/148‑1 (MQ=255)

                                                                                                                                        ttAGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGCAAAAGCAGaacaa       <  2:686652/150‑1 (MQ=255)

                                                                                                                                          aGGCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGCAAAAGCAGAACAAccc    >  3:74508/1‑151 (MQ=255)

                                                                                                                                           ggCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGCAAAAGCAGAACAACCCa   <  3:130861/151‑1 (MQ=255)

                                                                                                                                            gCTTGATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGCAAAAGCAGAACAACCCAt  <  4:183853/151‑1 (MQ=255)

TCACATTGATGACAACCACGCTGTTATCAAAATGACGATTATGGATTCTGGAAGTGGATTATCGCAGGAAGAACAACAACAACTGTTTAAACGCTACAGCCAAACAAGTGCAGGTCGTCAGCAAACAGGTTCTGGTTTAGGCTTAATGATCTGCAAAGAATTAATTAAAAATATGCAGGGCGATTTGTCATTAGAAAGTCATCCAGGCATAGGAACAACATTTACGATCACAATCCCGGTAGAAATTAGCCAGCAAGTGGCGACTGTCGAGGCAAAAGCAGAACAACCCAT  >  NC_000913/2486941‑2487231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`