Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,536,495 G→A A248V (GCG→GTG)  pdxK ← pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,536,4950GA100.0% 460.8 / NA 121A248V (GCG→GTG) pdxKpyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (64/57);  total (64/57)

Only 100 of 121 total aligned reads displayed.

TTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCATTAC  >  NC_000913/2536347‑2536642
                                                                                                                                                    |                                                                                                                                                   
 tGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACAcc                                                                                                                                                   <  2:154655/150‑1 (MQ=255)
  gCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCtt                                                                                                                                                 <  3:839753/151‑1 (MQ=255)
   ccGCAGTGAAAAATGGCGCCCATCGGCGCCATTGTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTc                                                                                                                                                <  2:25986/151‑1 (MQ=255)
    cGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTcc                                                                                                                                               <  3:1362/151‑1 (MQ=255)
     gCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTccc                                                                                                                                              >  3:216604/1‑151 (MQ=255)
       aGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCtt                                                                                                                                            >  4:72403/1‑151 (MQ=255)
             aaaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGc                                                                                                                                       >  2:1045624/1‑150 (MQ=255)
              aaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCa                                                                                                                                      >  3:719512/1‑150 (MQ=255)
                    gcCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCCTCGGTTAACACCTTCCCTTTCAGCAAGCCAc                                                                                                                                >  3:812484/1‑150 (MQ=255)
                     cccATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtg                                                                                                                              >  2:650478/1‑151 (MQ=255)
                     cccATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtg                                                                                                                              <  2:1265693/151‑1 (MQ=255)
                      ccATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtg                                                                                                                              >  1:179031/1‑150 (MQ=255)
                       cATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtgat                                                                                                                            <  2:406459/151‑1 (MQ=255)
                           ggCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCGCTGAtgagc                                                                                                                        >  2:862055/1‑151 (MQ=255)
                            gcgcCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagct                                                                                                                       <  2:836677/151‑1 (MQ=255)
                            gcgcCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagct                                                                                                                       >  3:242487/1‑151 (MQ=255)
                             cgcCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagctg                                                                                                                      >  2:61212/1‑151 (MQ=255)
                             cgcCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACCGAtgagctg                                                                                                                      <  3:644435/151‑1 (MQ=255)
                              gcCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagctga                                                                                                                     <  2:586790/151‑1 (MQ=255)
                               ccATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagctgag                                                                                                                    <  2:968595/151‑1 (MQ=255)
                                cATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagctgagc                                                                                                                   <  2:150053/151‑1 (MQ=255)
                                  tttttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGcac                                                                                                                 >  3:1057565/1‑151 (MQ=255)
                                   ttttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGcac                                                                                                                 <  3:61223/150‑1 (MQ=255)
                                   ttttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGcac                                                                                                                 <  1:465829/150‑1 (MQ=255)
                                   ttttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGcac                                                                                                                 >  4:19548/1‑150 (MQ=255)
                                     ttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACaa                                                                                                               >  2:973600/1‑150 (MQ=255)
                                     ttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACaa                                                                                                               >  3:1255391/1‑150 (MQ=255)
                                        tATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAAc                                                                                                            >  3:446893/1‑150 (MQ=255)
                                        tATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAAc                                                                                                            >  1:453867/1‑150 (MQ=255)
                                        tATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAAc                                                                                                            >  2:878693/1‑150 (MQ=255)
                                          tGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAgg                                                                                                         >  3:395473/1‑151 (MQ=255)
                                            cTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTc                                                                                                       >  3:137545/1‑151 (MQ=255)
                                                cGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCa                                                                                                   <  2:332070/151‑1 (MQ=255)
                                                 gCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGcc                                                                                                    >  2:736800/1‑149 (MQ=255)
                                                  ccAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGt                                                                                                 >  2:650868/1‑151 (MQ=255)
                                                   cAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTc                                                                                                >  2:876990/1‑151 (MQ=255)
                                                   cAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTc                                                                                                >  2:780166/1‑151 (MQ=255)
                                                    aGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTcc                                                                                               <  3:1054776/151‑1 (MQ=255)
                                                     gcggcgGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAGCCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTc                                                                                                <  2:1054753/149‑1 (MQ=255)
                                                     gcggcgGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTccc                                                                                              <  2:1008685/151‑1 (MQ=255)
                                                       ggcggcAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCtt                                                                                            >  3:1347829/1‑151 (MQ=255)
                                                       ggcggcAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCtt                                                                                            >  3:279215/1‑151 (MQ=255)
                                                         cggcAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTc                                                                                          >  2:1305290/1‑151 (MQ=255)
                                                          ggcAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCa                                                                                         <  3:932270/151‑1 (MQ=255)
                                                             aaaaTCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGt                                                                                      <  4:376880/151‑1 (MQ=255)
                                                             aaaaTCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGt                                                                                      >  3:11111/1‑151 (MQ=255)
                                                              aaaTCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTc                                                                                     >  1:180090/1‑151 (MQ=255)
                                                               aaTCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCg                                                                                    <  1:40840/151‑1 (MQ=255)
                                                               aaTCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCg                                                                                    >  3:1123187/1‑151 (MQ=255)
                                                                aTCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCgg                                                                                   <  2:367408/151‑1 (MQ=255)
                                                                aTCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCgg                                                                                   <  2:137529/151‑1 (MQ=255)
                                                                  cAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGt                                                                                  <  2:348920/150‑1 (MQ=255)
                                                                   aaTTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGttt                                                                                >  3:1301201/1‑151 (MQ=255)
                                                                       cATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTAc                                                                            <  1:72403/151‑1 (MQ=255)
                                                                        aTCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTAcc                                                                           >  2:196687/1‑151 (MQ=255)
                                                                          cGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCg                                                                         <  3:641886/151‑1 (MQ=255)
                                                                           gCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCg                                                                         >  3:171550/1‑150 (MQ=255)
                                                                           gCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGt                                                                        <  2:768739/151‑1 (MQ=255)
                                                                             tctcATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCGCTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTg                                                                       >  3:466796/1‑150 (MQ=255)
                                                                                cATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAAt                                                                    <  2:242462/150‑1 (MQ=255)
                                                                                 aTGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATgg                                                                  <  3:428665/151‑1 (MQ=255)
                                                                                 aTGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATgg                                                                  >  2:2216/1‑151 (MQ=255)
                                                                                      gctgGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGaaa                                                              <  2:1014477/150‑1 (MQ=255)
                                                                                      gctgGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAAt                                                             <  3:844824/151‑1 (MQ=255)
                                                                                      gctgGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAAt                                                             <  1:152854/151‑1 (MQ=255)
                                                                                      gctgGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAAt                                                             >  3:564627/1‑151 (MQ=255)
                                                                                      gctgGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAAt                                                             <  2:842501/151‑1 (MQ=255)
                                                                                       ctgGGTGTAGCGCATCCCTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCTGCAAGCCACTGATGAGCTGAGCCTAAAACCGGTCGCCAGTCCCTTTCAGGTCTGTTTTTACCCGTGAATGGGaa                                                               <  3:1388039/148‑1 (MQ=255)
                                                                                        tgGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATg                                                            >  4:85754/1‑150 (MQ=255)
                                                                                        tgGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATg                                                            >  3:1289535/1‑150 (MQ=255)
                                                                                         gggTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGAc                                                          >  4:453337/1‑151 (MQ=255)
                                                                                          ggTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACa                                                         <  2:137187/151‑1 (MQ=255)
                                                                                           gtgtAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACAt                                                        >  2:435762/1‑151 (MQ=255)
                                                                                               cgcgcATCACTTCCAGTACGCGCAACCCCGCCCGGCGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCAc                                                    >  4:479693/2‑151 (MQ=255)
                                                                                                   cATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTg                                                <  1:109823/151‑1 (MQ=255)
                                                                                                    aTCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGt                                               >  4:399573/1‑151 (MQ=255)
                                                                                                    aTCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGt                                               >  2:159073/1‑151 (MQ=255)
                                                                                                       aCTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCgg                                            >  4:322369/1‑151 (MQ=255)
                                                                                                        cTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGc                                           >  1:181391/1‑151 (MQ=255)
                                                                                                          tCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGc                                           <  4:181389/149‑1 (MQ=255)
                                                                                                           ccAGTACGCGCAACCCCGCTCGGAGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGCCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGt                                        >  4:56713/1‑151 (MQ=255)
                                                                                                             aGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTg                                       >  3:236697/1‑150 (MQ=255)
                                                                                                             aGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGa                                      <  3:736831/151‑1 (MQ=255)
                                                                                                              gTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGac                                     <  2:252237/151‑1 (MQ=255)
                                                                                                                aCGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGacca                                   <  3:1388417/151‑1 (MQ=255)
                                                                                                                      aaCCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACaa                             <  2:247657/151‑1 (MQ=255)
                                                                                                                          ccGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTg                         >  4:301127/1‑151 (MQ=255)
                                                                                                                            gCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGc                        >  3:1356671/1‑150 (MQ=255)
                                                                                                                               cGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCAtct                    <  3:180911/151‑1 (MQ=255)
                                                                                                                               cGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCAtct                    >  4:244533/1‑151 (MQ=255)
                                                                                                                                  tGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCt                 >  3:1389786/1‑151 (MQ=255)
                                                                                                                                    cACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGa               <  3:1149157/151‑1 (MQ=255)
                                                                                                                                      cTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCGGTGACCACCACAACCTGCATCTCCTGAt              <  2:812450/150‑1 (MQ=255)
                                                                                                                                         cATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTttc          >  1:65717/1‑151 (MQ=255)
                                                                                                                                             ggTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCa      <  2:1389716/151‑1 (MQ=255)
                                                                                                                                             ggTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCa      <  3:780201/151‑1 (MQ=255)
                                                                                                                                                tAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCAtt    >  3:1027000/1‑150 (MQ=255)
                                                                                                                                                tAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCAtt    >  3:114203/1‑150 (MQ=255)
                                                                                                                                                 aaCACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCATTa   <  1:314853/150‑1 (MQ=255)
                                                                                                                                                 aaCACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCATTAc  >  2:664098/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCATTAC  >  NC_000913/2536347‑2536642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑