Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,809,534 T→C intergenic (+41/‑83) ygaY → / → ygaZ pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein/putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,809,5340TC98.2% 402.0 / ‑4.1 111intergenic (+41/‑83)ygaY/ygaZpseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein/putative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (47/62);  total (48/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAG  >  NC_000913/2809387‑2809681
                                                                                                                                                   |                                                                                                                                                   
ggCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGtt                                                                                                                                                  <  2:1255873/151‑1 (MQ=255)
ggCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGtt                                                                                                                                                  <  1:395311/151‑1 (MQ=255)
  cAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTaa                                                                                                                                                <  2:1040623/151‑1 (MQ=255)
  cAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGGCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTTCACTTATAAGGGCGTTa                                                                                                                                                 >  2:581670/1‑150 (MQ=255)
   aaCATGGCGGTTGGGCTGGCGGTTTTCTGGCTGGCGCGACGGATTCCCTGGTTAACTTACTGGGCTGGTGGGGGGGGTTTCATTGTCAGGGAACCGCCAATTAAGCAATCAGCCACCGGTTTCGTGGCTTTACACTTATAAAGGCGTTaa                                                                                                                                                >  3:650807/1‑150 (MQ=255)
    aCATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTa                                                                                                                                                 >  2:932994/1‑148 (MQ=255)
     cATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTaagaa                                                                                                                                             <  1:167664/151‑1 (MQ=255)
              tGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATc                                                                                                                                      >  3:1118482/1‑149 (MQ=255)
                ggCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTc                                                                                                                                  <  3:1367108/151‑1 (MQ=255)
                  cTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTg                                                                                                                                >  3:484827/1‑151 (MQ=255)
                     gCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGAt                                                                                                                              >  2:1311194/1‑150 (MQ=255)
                       gTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATa                                                                                                                             >  2:1277501/1‑149 (MQ=255)
                       gTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAg                                                                                                                           <  3:114627/151‑1 (MQ=255)
                        tttGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAgg                                                                                                                          >  2:654351/1‑151 (MQ=255)
                         ttGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGt                                                                                                                         >  3:404094/1‑151 (MQ=255)
                          tGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGt                                                                                                                         <  2:541103/150‑1 (MQ=255)
                           gTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGtt                                                                                                                        <  3:470454/150‑1 (MQ=255)
                           gTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTa                                                                                                                       <  2:47694/151‑1 (MQ=255)
                             ctggctggCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTa                                                                                                                       >  4:46317/1‑149 (MQ=255)
                               ggctggcGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGTTTAAGa                                                                                                                    <  3:832913/150‑1 (MQ=255)
                               ggctggcGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTaa                                                                                                                      <  4:222787/148‑1 (MQ=255)
                               ggctggcGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGat                                                                                                                   <  2:1039455/151‑1 (MQ=255)
                               ggctggcGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGa                                                                                                                    <  3:182805/150‑1 (MQ=255)
                               ggctggcGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGa                                                                                                                    <  2:291259/150‑1 (MQ=255)
                                gctggcGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGata                                                                                                                  <  2:622647/151‑1 (MQ=255)
                                  tggcGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATatt                                                                                                                >  3:578165/1‑151 (MQ=255)
                                  tggcGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATatt                                                                                                                >  3:1183648/1‑151 (MQ=255)
                                   ggcGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGatat                                                                                                                 <  3:184455/149‑1 (MQ=255)
                                   ggcGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATattc                                                                                                               <  4:405315/151‑1 (MQ=255)
                                     cgcgACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATattcat                                                                                                             >  4:120145/1‑151 (MQ=255)
                                      gcgACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATattcatt                                                                                                            <  3:337877/151‑1 (MQ=255)
                                       cgacgaTTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATattcattc                                                                                                           <  3:548560/151‑1 (MQ=255)
                                        gacgaTTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATattcattc                                                                                                           >  1:258487/1‑150 (MQ=255)
                                          cgaTTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGt                                                                                                        <  2:188147/151‑1 (MQ=255)
                                           gaTTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTc                                                                                                       >  3:738809/1‑151 (MQ=255)
                                            aTTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCt                                                                                                      <  2:1216000/151‑1 (MQ=255)
                                                  cTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTtat                                                                                                >  2:1211923/1‑151 (MQ=255)
                                                   tGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTtat                                                                                                >  1:306253/1‑150 (MQ=255)
                                                    ggTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTtata                                                                                               <  2:484793/150‑1 (MQ=255)
                                                    ggTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTtata                                                                                               <  2:1140979/150‑1 (MQ=255)
                                                    ggTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTataa                                                                                              <  2:593349/151‑1 (MQ=255)
                                                     gTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCGGTCTATTTataat                                                                                             <  2:795530/151‑1 (MQ=255)
                                                      ttAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTataat                                                                                             >  3:29429/1‑150 (MQ=255)
                                                          cTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTa                                                                                         >  3:301427/1‑150 (MQ=255)
                                                                ggtctggtGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAAt                                                                                    <  2:948952/149‑1 (MQ=255)
                                                                 gtctggtGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGt                                                                                 <  4:102042/151‑1 (MQ=255)
                                                                 gtctggtGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGt                                                                                 <  2:853456/151‑1 (MQ=255)
                                                                   ctggtGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGtt                                                                                >  1:281177/1‑150 (MQ=255)
                                                                     ggtggCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTa                                                                               <  3:1093765/149‑1 (MQ=255)
                                                                     ggtggCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTa                                                                               >  2:1192591/1‑149 (MQ=255)
                                                                     ggtggCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTa                                                                               <  2:904624/149‑1 (MQ=255)
                                                                     ggtggCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTa                                                                               <  4:281094/149‑1 (MQ=255)
                                                                     ggtggCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTa                                                                               <  4:306250/149‑1 (MQ=255)
                                                                     ggtggCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAg                                                                             <  3:802583/151‑1 (MQ=255)
                                                                      gtggCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGc                                                                            <  2:650782/151‑1 (MQ=255)
                                                                       tggCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCg                                                                           >  3:767112/1‑151 (MQ=255)
                                                                         gCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTa                                                                         <  3:1278905/151‑1 (MQ=255)
                                                                           gAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTac                                                                        >  4:329728/1‑150 (MQ=255)
                                                                             ggTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACAct                                                                     <  3:124193/151‑1 (MQ=255)
                                                                                  tCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTAt                                                                 <  3:581701/150‑1 (MQ=255)
                                                                                  tCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTAt                                                                 <  3:710109/150‑1 (MQ=255)
                                                                                   cATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATgg                                                               >  2:1232775/1‑151 (MQ=255)
                                                                                   cATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATgg                                                               >  2:652433/1‑151 (MQ=255)
                                                                                   cATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATg                                                                <  2:939264/150‑1 (MQ=255)
                                                                                      cGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGaa                                                             >  2:721669/1‑150 (MQ=255)
                                                                                       gTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAg                                                           <  2:642068/151‑1 (MQ=255)
                                                                                          aGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCAACAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGccc                                                        >  3:163315/1‑151 (MQ=255)
                                                                                             aaGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTAc                                                     >  1:451659/1‑151 (MQ=255)
                                                                                              aGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACt                                                    >  2:77258/1‑151 (MQ=255)
                                                                                                    aaaTTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCcac                                                >  1:456213/1‑149 (MQ=255)
                                                                                                    aaaTTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCcac                                                >  2:576177/1‑149 (MQ=255)
                                                                                                        tAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTg                                          >  2:581847/1‑151 (MQ=255)
                                                                                                          aGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCt                                        >  2:911997/1‑151 (MQ=255)
                                                                                                            cAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTcc                                      <  3:457000/151‑1 (MQ=255)
                                                                                                            cAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTcc                                      <  2:282097/151‑1 (MQ=255)
                                                                                                            cAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTcc                                      >  2:881806/1‑151 (MQ=255)
                                                                                                              aTCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTg                                    >  2:1371350/1‑151 (MQ=255)
                                                                                                                cAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGt                                  >  1:214105/1‑151 (MQ=255)
                                                                                                                cAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGt                                  <  2:404063/151‑1 (MQ=255)
                                                                                                                    aaCCGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCgg                              >  3:793504/1‑151 (MQ=255)
                                                                                                                      ccGTTTTCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGc                             <  2:434774/150‑1 (MQ=255)
                                                                                                                         ttttCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGAcc                         >  2:965692/1‑151 (MQ=255)
                                                                                                                           ttCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCtt                       >  3:730348/1‑151 (MQ=255)
                                                                                                                           ttCGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCt                        <  3:419445/150‑1 (MQ=255)
                                                                                                                             cGTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTc                      <  2:773053/150‑1 (MQ=255)
                                                                                                                              gTGGCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCAt                    >  3:663634/1‑151 (MQ=255)
                                                                                                                                ggCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATgg                  >  3:822014/1‑151 (MQ=255)
                                                                                                                                ggCTTTACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATg                   <  3:1192648/150‑1 (MQ=255)
                                                                                                                                   tttACACTTATAAGGGTGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGa                 >  3:1267524/1‑149 (MQ=255)
                                                                                                                                   tttACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGa                 >  3:1377107/1‑149 (MQ=255)
                                                                                                                                    ttACACTTATAAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAg               <  4:214103/150‑1 (MQ=255)
                                                                                                                                           tataAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCaa        <  2:239082/150‑1 (MQ=255)
                                                                                                                                           tataAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCaa        <  3:1277567/150‑1 (MQ=255)
                                                                                                                                           tataAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCa         <  2:1105599/149‑1 (MQ=255)
                                                                                                                                            ataAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCaaa       <  2:738777/150‑1 (MQ=255)
                                                                                                                                            ataAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCaaa       <  2:1022856/150‑1 (MQ=255)
                                                                                                                                            ataAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCaa        >  1:467377/1‑149 (MQ=255)
                                                                                                                                            ataAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCaa        >  1:174837/1‑149 (MQ=255)
                                                                                                                                             taAGGGCGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCaa        >  2:308253/1‑148 (MQ=255)
                                                                                                                                                gggTGTTAAGAAGCGCATCAGTATGATAAGGTTAAGATATTCATTCAGTCTATTTATAAGATTAACAATCGTTTATTGTTCAATCTATGGACAGCCCCCCTCCTCAGCCTGCTAGTGGTTCCGCTTCCCCCATCGAAGGCTGctcagatac  <  3:110129/151‑9 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAG  >  NC_000913/2809387‑2809681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: