Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,825,128 A→G V150A (GTA→GCA)  mltB ← membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,825,1280AG100.0% 390.2 / NA 106V150A (GTA→GCA) mltBmembrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/56);  total (50/56)

Only 100 of 106 total aligned reads displayed.

GAAGGTTTCCAGCTCGCCAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGG  >  NC_000913/2824980‑2825275
                                                                                                                                                    |                                                                                                                                                   
gAAGGTTTCCAGCTCGCCAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCt                                                                                                                                                   <  3:93510/151‑1 (MQ=255)
 aaGGTTTCCAGCTCGCCAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTc                                                                                                                                                  >  4:442148/1‑151 (MQ=255)
    gTTTCCAGCTCGCCAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCAt                                                                                                                                               <  1:13949/151‑1 (MQ=255)
    gTTTCCAGCTCGCCAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCAt                                                                                                                                               <  3:980539/151‑1 (MQ=255)
        ccAGCTCGCCAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATaca                                                                                                                                            <  3:790319/150‑1 (MQ=255)
         cAGCTCGCCAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACAcc                                                                                                                                          <  3:951114/151‑1 (MQ=255)
          aGCTCGCCAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCt                                                                                                                                         <  2:607896/151‑1 (MQ=255)
           gCTCGCCAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTg                                                                                                                                        <  1:130895/151‑1 (MQ=255)
           gCTCGCCAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTg                                                                                                                                        >  3:1279691/1‑151 (MQ=255)
            cTCGCCAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGc                                                                                                                                       >  2:562334/1‑151 (MQ=255)
                ccAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCAcg                                                                                                                                   >  3:408626/1‑151 (MQ=255)
                 cAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCAcgc                                                                                                                                  >  1:472626/1‑151 (MQ=255)
                  agagAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCAcgcg                                                                                                                                 >  3:878840/1‑151 (MQ=255)
                   gagaAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCAcgcgc                                                                                                                                <  2:655987/151‑1 (MQ=255)
                   gagaAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCAcgcgc                                                                                                                                <  2:448749/151‑1 (MQ=255)
                    agaAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCAcgcgcg                                                                                                                               >  3:1111429/1‑151 (MQ=255)
                    agaAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCAcgcgcg                                                                                                                               <  2:615084/151‑1 (MQ=255)
                     gaAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGa                                                                                                                              <  1:147730/151‑1 (MQ=255)
                      aaaTACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGAt                                                                                                                             >  1:211080/1‑151 (MQ=255)
                      aaaTACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGAt                                                                                                                             >  1:445284/1‑151 (MQ=255)
                       aaTACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGAtt                                                                                                                            >  2:830976/1‑151 (MQ=255)
                          aCTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGAtt                                                                                                                            >  2:1092923/1‑148 (MQ=255)
                          aCTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCa                                                                                                                          <  3:442008/150‑1 (MQ=255)
                                cgcgGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCAt                                                                                                                    >  3:1232039/1‑150 (MQ=255)
                                cgcgGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATc                                                                                                                   >  2:626414/1‑151 (MQ=255)
                                cgcgGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATc                                                                                                                   >  1:205369/1‑151 (MQ=255)
                                 gcggcgTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCt                                                                                                                  <  2:1371331/151‑1 (MQ=255)
                                 gcggcgTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCt                                                                                                                  <  2:315134/151‑1 (MQ=255)
                                    gcgTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCa                                                                                                               <  3:1072615/151‑1 (MQ=255)
                                      gTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATa                                                                                                             <  3:573063/151‑1 (MQ=255)
                                      gTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATa                                                                                                             <  3:628191/151‑1 (MQ=255)
                                        gggTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCt                                                                                                                                         >  3:1001979/1‑121 (MQ=255)
                                            aGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGAtt                                                                                                       <  2:994592/151‑1 (MQ=255)
                                              ttAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTcc                                                                                                     >  1:106614/1‑151 (MQ=255)
                                               tAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTcc                                                                                                     <  2:481246/150‑1 (MQ=255)
                                                aaaTGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCa                                                                                                    <  4:360794/150‑1 (MQ=255)
                                                  aTGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGaa                                                                                                 <  3:175093/151‑1 (MQ=255)
                                                   tGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGaa                                                                                                 >  1:421343/1‑150 (MQ=255)
                                                    gACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGaaa                                                                                                >  2:685834/1‑150 (MQ=255)
                                                      cAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAAcc                                                                                             <  3:1378857/151‑1 (MQ=255)
                                                       aGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACca                                                                                            <  4:415089/151‑1 (MQ=255)
                                                        gCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGCAAACcac                                                                                           >  4:57925/1‑151 (MQ=255)
                                                        gCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACcac                                                                                           >  3:1064378/1‑151 (MQ=255)
                                                        gCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACcac                                                                                           <  1:171454/151‑1 (MQ=255)
                                                         cGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACcaca                                                                                          <  1:57087/151‑1 (MQ=255)
                                                            tGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCg                                                                                       >  1:417912/1‑151 (MQ=255)
                                                            tGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCg                                                                                       >  3:404967/1‑151 (MQ=255)
                                                            tGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGCGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCg                                                                                       >  3:365270/1‑151 (MQ=255)
                                                              ccAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGtt                                                                                     <  2:578673/151‑1 (MQ=255)
                                                              ccAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGtt                                                                                     <  3:766484/151‑1 (MQ=255)
                                                                aGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCt                                                                                   <  3:722159/151‑1 (MQ=255)
                                                                 gcgcATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTg                                                                                  <  3:583393/151‑1 (MQ=255)
                                                                 gcgcATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTg                                                                                  >  2:639124/1‑151 (MQ=255)
                                                                     aTCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACg                                                                              <  1:157085/151‑1 (MQ=255)
                                                                       cGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGtt                                                                            >  2:1280077/1‑151 (MQ=255)
                                                                        gAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTg                                                                           <  2:1111376/151‑1 (MQ=255)
                                                                            aTGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCg                                                                       <  2:1279625/151‑1 (MQ=255)
                                                                            aTGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCAGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCg                                                                       <  2:14758/151‑1 (MQ=255)
                                                                              gCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGc                                                                     >  1:78871/1‑151 (MQ=255)
                                                                               cGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCg                                                                    <  2:461204/151‑1 (MQ=255)
                                                                                  gTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGtaa                                                                 <  2:781101/151‑1 (MQ=255)
                                                                                     ttCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGtaata                                                               >  2:1375261/1‑150 (MQ=255)
                                                                                      tCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGtaataa                                                              >  2:487308/1‑150 (MQ=255)
                                                                                       ccccATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATaaa                                                             <  4:416166/150‑1 (MQ=255)
                                                                                       ccccATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAAt                                                            >  3:303343/1‑151 (MQ=255)
                                                                                        cccATCCCGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACCCCTGCCACGCGCGATTCAACGCATCTTCCTACTGATTTCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAAtt                                                           <  3:875900/151‑1 (MQ=255)
                                                                                        cccATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAAtt                                                           >  2:1305485/1‑151 (MQ=255)
                                                                                         ccATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAAttt                                                          <  2:297794/151‑1 (MQ=255)
                                                                                           aTCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAAttttt                                                        <  3:702222/151‑1 (MQ=255)
                                                                                           aTCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAAttttt                                                        <  1:373469/151‑1 (MQ=255)
                                                                                            tCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAAttt                                                          <  2:655691/148‑1 (MQ=255)
                                                                                              aCGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGc                                                     >  3:111651/1‑151 (MQ=255)
                                                                                              aCGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGc                                                     >  2:278036/1‑151 (MQ=255)
                                                                                               cgcgCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCg                                                    <  3:1066196/151‑1 (MQ=255)
                                                                                               cgcgCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGAGAATTTCCGGCGGTGCTCCATACACCTGCCCCGCGCGATTCAACGCATCGTCATACTGATTCCAGAAAACCGCACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCg                                                    <  3:57238/151‑1 (MQ=255)
                                                                                                  gcCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGAt                                                  >  2:944133/1‑150 (MQ=255)
                                                                                                  gcCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGAt                                                  >  3:877074/1‑150 (MQ=255)
                                                                                                  gcCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATa                                                 <  3:872988/151‑1 (MQ=255)
                                                                                                   cccccAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATaa                                                <  3:856872/151‑1 (MQ=255)
                                                                                                   cccccAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATa                                                 <  3:84500/150‑1 (MQ=255)
                                                                                                    ccccAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATaa                                                >  2:1273242/1‑150 (MQ=255)
                                                                                                    ccccAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTGTGCGATAAc                                               >  1:49021/1‑151 (MQ=255)
                                                                                                      ccAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACgg                                             >  4:107303/1‑151 (MQ=255)
                                                                                                        aGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAg                                           <  4:29828/151‑1 (MQ=255)
                                                                                                         gCGGGTTTCAAGGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACACGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACg                                              >  3:716556/1‑147 (MQ=255)
                                                                                                          cGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGcc                                         <  2:508711/151‑1 (MQ=255)
                                                                                                              tttCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGCTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATg                                      >  3:701561/1‑150 (MQ=255)
                                                                                                               ttCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATgcg                                    >  2:111985/1‑151 (MQ=255)
                                                                                                                tCAACGCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATgcg                                    <  1:98453/150‑1 (MQ=255)
                                                                                                                     gCCGATAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTc                              >  2:967636/1‑151 (MQ=255)
                                                                                                                         aTAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGAc                          >  3:1113328/1‑151 (MQ=255)
                                                                                                                         aTAATCCCGACGATAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGAc                          <  2:303314/151‑1 (MQ=255)
                                                                                                                                 gacgaTAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGc                  >  2:176711/1‑151 (MQ=255)
                                                                                                                                 gacgaTAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGc                  <  3:1002205/151‑1 (MQ=255)
                                                                                                                                  acgaTAATTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCg                 >  2:215097/1‑151 (MQ=255)
                                                                                                                                       aaTTTCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGttt             >  3:750610/1‑150 (MQ=255)
                                                                                                                                           tCCGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCg        >  3:431174/1‑151 (MQ=255)
                                                                                                                                            ccGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCg        >  3:1002162/1‑150 (MQ=255)
                                                                                                                                            ccGGCGGTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGa       <  2:1076375/151‑1 (MQ=255)
                                                                                                                                              ggcggTGCTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGAtg     >  3:1226106/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAAGGTTTCCAGCTCGCCAGAGAAATACTCCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGG  >  NC_000913/2824980‑2825275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑