Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,913,035 A→G L206P (CTC→CCC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,913,0350AG99.1% 415.3 / NA 106L206P (CTC→CCC) relA(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (56/49);  total (57/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

GTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAAT  >  NC_000913/2912887‑2913183
                                                                                                                                                    |                                                                                                                                                    
gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGgggg                                                                                                                                                    <  2:732235/151‑1 (MQ=255)
gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGgggg                                                                                                                                                    <  3:545384/151‑1 (MQ=255)
gtgtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGgggg                                                                                                                                                    >  4:436812/1‑151 (MQ=255)
  gtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGt                                                                                                                                                   <  1:248840/150‑1 (MQ=255)
  gtTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGt                                                                                                                                                   >  3:1150765/1‑150 (MQ=255)
    ttCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAAc                                                                                                                                                >  3:922181/1‑151 (MQ=255)
      cGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACgg                                                                                                                                              >  3:300433/1‑151 (MQ=255)
       ggACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTTTTCGGTTGGATGGGGGTAACgg                                                                                                                                              >  2:884039/1‑150 (MQ=255)
       ggACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACgg                                                                                                                                              >  2:451627/1‑150 (MQ=255)
        gacgacCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGaa                                                                                                                                            <  4:129771/151‑1 (MQ=255)
          cgacCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGc                                                                                                                                          >  2:603452/1‑151 (MQ=255)
           gacCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCa                                                                                                                                         <  3:220064/151‑1 (MQ=255)
           gacCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCa                                                                                                                                         <  3:330920/151‑1 (MQ=255)
               aTACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTaa                                                                                                                                     <  2:1334203/151‑1 (MQ=255)
                tACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTaa                                                                                                                                     <  3:1404047/150‑1 (MQ=255)
                 acacTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATc                                                                                                                                   <  3:339027/151‑1 (MQ=255)
                      tCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTcc                                                                                                                               >  1:145328/1‑150 (MQ=255)
                       ccGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCa                                                                                                                              <  3:471754/150‑1 (MQ=255)
                       ccGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCa                                                                                                                              <  2:78908/150‑1 (MQ=255)
                        cGCTTTAACGCCATCAGCTTTCATCTCAACTCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTATGTATTCGGCTGGATGAAGGTAACGGAAGCAGTAATCTTCCAGt                                                                                                                            >  3:303544/1‑151 (MQ=255)
                         gCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGtt                                                                                                                           >  1:105820/1‑151 (MQ=255)
                         gCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGtt                                                                                                                           >  2:396569/1‑151 (MQ=255)
                           tttAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTcc                                                                                                                         >  3:1286905/1‑151 (MQ=255)
                            ttAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTc                                                                                                                          <  1:196544/149‑1 (MQ=255)
                              aaCGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCAt                                                                                                                      >  2:85027/1‑151 (MQ=255)
                                cGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCAtt                                                                                                                     >  3:900725/1‑150 (MQ=255)
                                  ccTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTc                                                                                                                   >  1:242393/1‑150 (MQ=255)
                                  ccTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCa                                                                                                                  <  1:197586/151‑1 (MQ=255)
                                      cAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGtt                                                                                                               <  2:1350179/150‑1 (MQ=255)
                                      cAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGtt                                                                                                               <  2:347210/150‑1 (MQ=255)
                                       aGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGt                                                                                                             <  4:304048/151‑1 (MQ=255)
                                       aGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGt                                                                                                             <  3:734323/151‑1 (MQ=255)
                                        gCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTc                                                                                                            <  2:448346/151‑1 (MQ=255)
                                         cTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTcc                                                                                                           >  1:127302/1‑151 (MQ=255)
                                            tCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGa                                                                                                         <  1:207831/150‑1 (MQ=255)
                                                  cAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTcc                                                                                                     <  2:38129/148‑1 (MQ=255)
                                                  cAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTc                                                                                                      <  2:321725/147‑1 (MQ=255)
                                                       cgcAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGt                                                                                             >  3:211932/1‑151 (MQ=255)
                                                       cgcAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGt                                                                                             >  2:1310711/1‑151 (MQ=255)
                                                         cAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTa                                                                                           <  3:875814/151‑1 (MQ=255)
                                                             tGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGcc                                                                                        >  2:71776/1‑150 (MQ=255)
                                                              gACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGcc                                                                                        >  4:100064/1‑149 (MQ=255)
                                                               aCCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCa                                                                                       <  2:1102808/149‑1 (MQ=255)
                                                                ccAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCg                                                                                    >  2:201992/1‑151 (MQ=255)
                                                                 cAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCgg                                                                                   >  3:1234366/1‑151 (MQ=255)
                                                                 cAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCgg                                                                                   >  1:366288/1‑151 (MQ=255)
                                                                   aCGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTg                                                                                 >  2:1163394/1‑151 (MQ=255)
                                                                   aCGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTg                                                                                 >  3:1293050/1‑151 (MQ=255)
                                                                     gAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCg                                                                               >  4:447146/1‑151 (MQ=255)
                                                                      aaCTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCg                                                                               >  2:857968/1‑150 (MQ=255)
                                                                       aCTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGt                                                                              >  3:1148389/1‑150 (MQ=255)
                                                                         tctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGt                                                                              >  3:239182/1‑148 (MQ=255)
                                                                         tctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGt                                                                              >  4:45381/1‑148 (MQ=255)
                                                                           ttCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATg                                                                         >  3:236721/1‑151 (MQ=255)
                                                                            tCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATg                                                                         <  3:307650/150‑1 (MQ=255)
                                                                             cGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGtt                                                                       <  4:18355/151‑1 (MQ=255)
                                                                             cGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGtt                                                                       <  2:1150709/151‑1 (MQ=255)
                                                                               aTGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTgg                                                                     <  1:466222/151‑1 (MQ=255)
                                                                                 gTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTa                                                                   <  3:319265/151‑1 (MQ=255)
                                                                                      gtTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACAct                                                               <  3:278797/150‑1 (MQ=255)
                                                                                         cgcgGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCtt                                                            <  2:239156/150‑1 (MQ=255)
                                                                                          gcgGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCtttt                                                          <  4:425146/151‑1 (MQ=255)
                                                                                          gcgGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCtttt                                                          >  3:604248/1‑151 (MQ=255)
                                                                                            ggTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTgc                                                        >  2:989406/1‑151 (MQ=255)
                                                                                            ggTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTgc                                                        <  2:1230724/151‑1 (MQ=255)
                                                                                               cGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTgccgc                                                     >  4:279919/1‑151 (MQ=255)
                                                                                               cGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTgccg                                                      <  3:441572/150‑1 (MQ=255)
                                                                                                 agaCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCa                                                   <  2:927182/151‑1 (MQ=255)
                                                                                                   aCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGt                                                 >  3:1186703/1‑151 (MQ=255)
                                                                                                   aCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGt                                                 >  2:50259/1‑151 (MQ=255)
                                                                                                     gCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTac                                               >  3:517050/1‑151 (MQ=255)
                                                                                                      ccGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTaca                                              <  2:1292981/151‑1 (MQ=255)
                                                                                                      ccGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTaca                                              <  2:653209/151‑1 (MQ=255)
                                                                                                        gTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACg                                            >  2:334564/1‑151 (MQ=255)
                                                                                                          tCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGt                                           <  2:300405/150‑1 (MQ=255)
                                                                                                            aTGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTc                                         >  4:455576/1‑150 (MQ=255)
                                                                                                            aTGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCa                                        <  2:832810/151‑1 (MQ=255)
                                                                                                            aTGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCa                                        <  1:77485/151‑1 (MQ=255)
                                                                                                               cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACActc                                                              <  1:292691/126‑1 (MQ=255)
                                                                                                                agcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCtt                                    <  3:603479/151‑1 (MQ=255)
                                                                                                                 gcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTc                                   >  2:848090/1‑151 (MQ=255)
                                                                                                                 gcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTc                                   <  4:105511/151‑1 (MQ=255)
                                                                                                                 gcagTTTGGAAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCt                                     <  3:884075/149‑1 (MQ=255)
                                                                                                                  cagTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTcc                                  >  3:155738/1‑151 (MQ=255)
                                                                                                                  cagTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTcc                                  <  1:422178/151‑1 (MQ=255)
                                                                                                                      ttGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGgcg                              >  2:1046859/1‑151 (MQ=255)
                                                                                                                          cAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATc                          >  3:1005393/1‑151 (MQ=255)
                                                                                                                           aaTTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCt                         >  3:939454/1‑151 (MQ=255)
                                                                                                                              tCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCttt                       <  3:50270/150‑1 (MQ=255)
                                                                                                                               cGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCtttt                      <  3:984271/150‑1 (MQ=255)
                                                                                                                               cGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTa                     <  2:1286837/151‑1 (MQ=255)
                                                                                                                               cGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTa                     <  2:1061509/151‑1 (MQ=255)
                                                                                                                                     tATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTcgc               >  1:440759/1‑151 (MQ=255)
                                                                                                                                        tCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCgcgc             >  2:100080/1‑150 (MQ=255)
                                                                                                                                          ggTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCgcgc             >  4:151970/1‑148 (MQ=255)
                                                                                                                                            ttGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATg        >  3:1333515/1‑151 (MQ=255)
                                                                                                                                             tggatgggGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATg        >  1:128842/1‑150 (MQ=255)
                                                                                                                                              ggatgggGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGa       <  4:420576/150‑1 (MQ=255)
                                                                                                                                                atgggGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCa    <  4:211622/151‑1 (MQ=255)
                                                                                                                                                   gaGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAAt  <  2:303514/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAAT  >  NC_000913/2912887‑2913183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: