Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,913,196 T→C K152K (AAA→AAG)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,913,1960TC100.0% 453.4 / NA 118K152K (AAA→AAG) relA(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (65/53);  total (65/53)

Only 100 of 118 total aligned reads displayed.

GTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACG  >  NC_000913/2913049‑2913343
                                                                                                                                                   |                                                                                                                                                   
gTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTtg                                                                                                                                                  <  2:591192/151‑1 (MQ=255)
       tCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTtgatg                                                                                                                                               <  4:272687/147‑1 (MQ=255)
         cAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACg                                                                                                                                         >  2:913110/1‑151 (MQ=255)
          aGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGc                                                                                                                                        >  3:1019169/1‑151 (MQ=255)
          aGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGc                                                                                                                                        >  3:920116/1‑151 (MQ=255)
           gTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCa                                                                                                                                       <  2:1148333/151‑1 (MQ=255)
            ttCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGc                                                                                                                                        >  1:249901/1‑149 (MQ=255)
               ccATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCg                                                                                                                                    >  2:181496/1‑150 (MQ=255)
                cATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGa                                                                                                                                   >  1:411101/1‑150 (MQ=255)
                 aTTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGaaa                                                                                                                                 <  2:1186646/151‑1 (MQ=255)
                 aTTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGaa                                                                                                                                  >  3:628239/1‑150 (MQ=255)
                 aTTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGaa                                                                                                                                  <  4:241027/150‑1 (MQ=255)
                    tCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAat                                                                                                                               <  1:312366/150‑1 (MQ=255)
                      aGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAatcat                                                                                                                            >  2:649728/1‑151 (MQ=255)
                        ttGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCg                                                                                                                          >  4:92117/1‑151 (MQ=255)
                         tGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCg                                                                                                                          <  1:112580/150‑1 (MQ=255)
                          gTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGAc                                                                                                                        >  4:323818/1‑151 (MQ=255)
                          gTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGAc                                                                                                                        >  4:331221/1‑151 (MQ=255)
                          gTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGAc                                                                                                                        <  2:1234307/151‑1 (MQ=255)
                            ccGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCa                                                                                                                      <  2:236695/151‑1 (MQ=255)
                             cGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCAt                                                                                                                     >  2:98964/1‑151 (MQ=255)
                              gATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATc                                                                                                                    >  4:455976/1‑151 (MQ=255)
                                ttCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCg                                                                                                                   <  2:922146/150‑1 (MQ=255)
                                ttCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGc                                                                                                                  >  3:676586/1‑151 (MQ=255)
                                  ccGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGcc                                                                                                                 >  2:828060/1‑150 (MQ=255)
                                  ccGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGcc                                                                                                                 >  2:848092/1‑150 (MQ=255)
                                    gagaCGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCaa                                                                                                               <  1:293810/150‑1 (MQ=255)
                                    gagaCGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAAt                                                                                                              <  3:85039/151‑1 (MQ=255)
                                      gaCGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATa                                                                                                             <  2:692755/150‑1 (MQ=255)
                                       aCGGTTAGCCAGCGGTGCGTAGATGGTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAAc                                                                                                           >  3:1057679/1‑151 (MQ=255)
                                        cGGTTAGCCAGCGGTGCGTAGATGTTGGTACTCTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCACCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACa                                                                                                          <  3:891798/151‑1 (MQ=255)
                                        cGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACa                                                                                                          <  3:773991/151‑1 (MQ=255)
                                         ggTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACAt                                                                                                         >  3:81913/1‑151 (MQ=255)
                                         ggTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACAt                                                                                                         <  4:210914/151‑1 (MQ=255)
                                          gTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATc                                                                                                        >  1:384519/1‑151 (MQ=255)
                                           ttAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATcc                                                                                                       >  2:1238018/1‑151 (MQ=255)
                                             aGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCgg                                                                                                     >  3:459968/1‑151 (MQ=255)
                                             aGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCgg                                                                                                     <  4:444236/151‑1 (MQ=255)
                                             aGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCg                                                                                                      <  3:1046905/150‑1 (MQ=255)
                                               ccAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCg                                                                                                   <  3:1280032/151‑1 (MQ=255)
                                                  gCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGaa                                                                                                 <  3:848126/150‑1 (MQ=255)
                                                  gCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAAc                                                                                                >  2:1303470/1‑151 (MQ=255)
                                                      tGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGtt                                                                                             >  2:367070/1‑150 (MQ=255)
                                                       gCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTAt                                                                                           >  1:2382/1‑151 (MQ=255)
                                                        cGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATc                                                                                          >  2:219839/1‑151 (MQ=255)
                                                        cGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATc                                                                                          >  3:15046/1‑151 (MQ=255)
                                                         gTAGTTGTTGGTGCTCTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAATATCATCGACCATCGCCAATAACATCCGGCGAACGTTAt                                                                                           <  4:229687/149‑1 (MQ=255)
                                                          tAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCg                                                                                         >  2:417932/1‑150 (MQ=255)
                                                                 tGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGtt                                                                                  <  2:517016/150‑1 (MQ=255)
                                                                 tGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTc                                                                                 >  1:284353/1‑151 (MQ=255)
                                                                    tACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCgg                                                                               >  2:479387/1‑150 (MQ=255)
                                                                     acacTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCgga                                                                              <  3:396601/150‑1 (MQ=255)
                                                                      cacTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCggagg                                                                            <  3:628265/151‑1 (MQ=255)
                                                                            tttGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAAc                                                                        <  3:181523/149‑1 (MQ=255)
                                                                             ttGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAg                                                                      <  2:10863/150‑1 (MQ=255)
                                                                               gccgccAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGaa                                                                    >  3:333299/1‑150 (MQ=255)
                                                                                ccgccAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATc                                                                  >  1:442452/1‑151 (MQ=255)
                                                                                 cgccAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATc                                                                  >  3:1308647/1‑150 (MQ=255)
                                                                                   ccAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAgt                                                               <  2:1333442/151‑1 (MQ=255)
                                                                                    cAGTACACGTTCATCTTCCGGCGCATCTTTTACTCCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAgtg                                                              >  1:258420/1‑151 (MQ=255)
                                                                                     aGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAgtgt                                                             >  2:147617/1‑151 (MQ=255)
                                                                                      gTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGtgtg                                                            >  2:434997/1‑151 (MQ=255)
                                                                                        acacGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCg                                                          <  2:155718/151‑1 (MQ=255)
                                                                                        acacGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCg                                                          >  3:814324/1‑151 (MQ=255)
                                                                                         cacGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGt                                                         <  2:1005351/151‑1 (MQ=255)
                                                                                          acGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTc                                                        <  1:58695/151‑1 (MQ=255)
                                                                                          acGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTc                                                        >  2:176582/1‑151 (MQ=255)
                                                                                           cGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTc                                                        <  3:100094/150‑1 (MQ=255)
                                                                                           cGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCg                                                       <  3:858005/151‑1 (MQ=255)
                                                                                               cATCTTGCGGCGCATCTTTTACTCCGCGCAGATGAGAAAGACGCTCCGCAAGCTTGATGACTACGCAGCGATAATCATCGACCATCGCCAATAACATCCGGCGATCGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCttc                                                   <  4:60658/151‑2 (MQ=255)
                                                                                               cATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCttt                                                   <  3:334592/151‑1 (MQ=255)
                                                                                                 tCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTc                                                  >  2:1116557/1‑150 (MQ=255)
                                                                                                 tCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTc                                                  >  3:1286767/1‑150 (MQ=255)
                                                                                                   ttCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGc                                               >  2:294702/1‑151 (MQ=255)
                                                                                                       ggCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGTCCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGc                                           <  3:202021/151‑1 (MQ=255)
                                                                                                         cgcATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCgg                                         >  3:658588/1‑151 (MQ=255)
                                                                                                            aTCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATc                                      >  3:394657/1‑151 (MQ=255)
                                                                                                             tCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcg                                     >  3:1233642/1‑151 (MQ=255)
                                                                                                              cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgc                                    >  2:836455/1‑151 (MQ=255)
                                                                                                               ttttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgcc                                   >  3:836009/1‑151 (MQ=255)
                                                                                                                tttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgccg                                  >  4:85273/1‑151 (MQ=255)
                                                                                                                 ttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgccgc                                 >  3:753389/1‑151 (MQ=255)
                                                                                                                  tACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCgccgcc                                >  3:1195719/1‑151 (MQ=255)
                                                                                                                      tCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCatat                            >  1:343600/1‑151 (MQ=255)
                                                                                                                      tCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCat                              <  3:1163450/149‑1 (MQ=255)
                                                                                                                       cgcgCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCatat                            <  3:1070442/150‑1 (MQ=255)
                                                                                                                         cgcAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATcac                         >  2:548014/1‑151 (MQ=255)
                                                                                                                          gcAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATcacg                        >  2:957115/1‑151 (MQ=255)
                                                                                                                            aGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATcacgca                      <  2:939416/151‑1 (MQ=255)
                                                                                                                             gATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATcacgcac                     <  2:1019125/151‑1 (MQ=255)
                                                                                                                              aTGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATcacgcac                     <  2:604221/150‑1 (MQ=255)
                                                                                                                                 aGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCg                 >  2:68073/1‑151 (MQ=255)
                                                                                                                                    aaTACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTg               >  2:1226200/1‑150 (MQ=255)
                                                                                                                                      tACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAAt            >  3:667749/1‑151 (MQ=255)
                                                                                                                                        cGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATa           <  3:913145/150‑1 (MQ=255)
                                                                                                                                          cTCCGCCAGCTTGATGACTCCGCAGCGAAAATCACCGACCACCGCCAATAACATCCGGCGAACGTTATCGACCGGTTCGGAGGAAACAGAATCAGTGTGCTTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAg         <  3:1000798/150‑1 (MQ=255)
                                                                                                                                          cTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGCCCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAg         <  2:1310263/150‑1 (MQ=255)
                                                                                                                                          cTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAgg        >  3:600407/1‑151 (MQ=255)
                                                                                                                                             cGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCAACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTa     <  3:219865/151‑1 (MQ=255)
                                                                                                                                                cAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAAcg  >  3:136600/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACG  >  NC_000913/2913049‑2913343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑