BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,103,474	G→A	W154* (TGG→TGA)	mutY →	adenine DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,103,474	0	G	A	100.0%	459.4 / NA	115	W154* (TGG→TGA)	mutY	adenine DNA glycosylase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (52/63); total (52/63)

Only 100 of 115 total aligned reads displayed.

CGGAAACCTTTGAGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGGCCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGTTCGCTCTGT  >  NC_000913/3103326‑3103618

cGGAAACCTTTGAGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGAcc                                                                                                                                                >  2:285469/1‑151 (MQ=255)

 ggAAACCTTTGAGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCt                                                                                                                                               >  3:142589/1‑151 (MQ=255)

 ggAAACCTTTGAGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCt                                                                                                                                               >  3:858406/1‑151 (MQ=255)

  gAAACCTTTGAGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTg                                                                                                                                              >  3:1030670/1‑151 (MQ=255)

    aaCCTTTGAGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTggg                                                                                                                                            >  3:904662/1‑151 (MQ=255)

     aCCTTTGAGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGa                                                                                                                                           <  3:1181232/151‑1 (MQ=255)

         ttGAGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGaaaa                                                                                                                                        <  2:424899/150‑1 (MQ=255)

          tGAGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGaaaaa                                                                                                                                       <  3:125892/150‑1 (MQ=255)

            aGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGaaaaa                                                                                                                                       >  2:891464/1‑148 (MQ=255)

            aGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAaga                                                                                                                                    <  2:766252/151‑1 (MQ=255)

              gAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAgg                                                                                                                                  <  4:383071/151‑1 (MQ=255)

              gAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAgg                                                                                                                                  <  2:1124386/151‑1 (MQ=255)

               aaGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGt                                                                                                                                 <  2:1253853/151‑1 (MQ=255)

                atttGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTc                                                                                                                                >  2:689792/3‑151 (MQ=255)

                 gTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCg                                                                                                                               <  3:1081331/151‑1 (MQ=255)

                 gTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCg                                                                                                                               <  3:1321185/151‑1 (MQ=255)

                  ttGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCg                                                                                                                               >  2:1077041/1‑150 (MQ=255)

                  ttGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCg                                                                                                                               >  3:204537/1‑150 (MQ=255)

                   tGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCg                                                                                                                               >  1:276441/1‑149 (MQ=255)

                    gcagcaCTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAACACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCgaga                                                                                                                            <  3:1054039/151‑1 (MQ=255)

                      agcaCTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGaa                                                                                                                           <  4:248608/150‑1 (MQ=255)

                        caCTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATa                                                                                                                         >  3:1250083/1‑150 (MQ=255)

                         aCTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATaa                                                                                                                        >  3:280511/1‑150 (MQ=255)

                         aCTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATaa                                                                                                                        >  2:999225/1‑150 (MQ=255)

                            gCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAAtt                                                                                                                     <  4:344512/150‑1 (MQ=255)

                              cgggcgtcgggcgtTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATg                                                                                                                  >  3:47939/1‑151 (MQ=255)

                               gggcgtcgggcgtTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATgg                                                                                                                 <  2:596600/151‑1 (MQ=255)

                                ggcgtcgggcgtTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGa                                                                                                                <  2:619981/151‑1 (MQ=255)

                                 gcgtcgggcgtTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATgg                                                                                                                 >  2:114313/1‑149 (MQ=255)

                                     cgggcgtTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGttt                                                                                                            <  3:844137/150‑1 (MQ=255)

                                     cgggcgtTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGttt                                                                                                            <  2:904627/150‑1 (MQ=255)

                                      gggcgtTCCACCGCAGGCGTGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTgg                                                                                                          <  3:989321/151‑2 (MQ=255)

                                       ggcgtTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTgag                                                                                                         <  3:1182572/151‑1 (MQ=255)

                                          gtTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTgagcga                                                                                                      <  1:123458/151‑1 (MQ=255)

                                          gtTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTgagcg                                                                                                       >  3:397482/1‑150 (MQ=255)

                                            tCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTgagcgagc                                                                                                    >  3:866138/1‑151 (MQ=255)

                                             ccACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTgagcgagc                                                                                                    <  1:39891/150‑1 (MQ=255)

                                               aCCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAgg                                                                                                 >  3:289385/1‑151 (MQ=255)

                                                ccGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGt                                                                                                <  2:869999/151‑1 (MQ=255)

                                                  gCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTg                                                                                               >  3:1258216/1‑150 (MQ=255)

                                                       cgcgATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGccc                                                                                         >  4:312136/1‑151 (MQ=255)

                                                       cgcgATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGccc                                                                                         <  3:912241/151‑1 (MQ=255)

                                                       cgcgATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGccc                                                                                         <  2:1317720/151‑1 (MQ=255)

                                                        gcgATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCcg                                                                                        <  2:1178625/151‑1 (MQ=255)

                                                         cgATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCcgc                                                                                       >  2:975674/1‑151 (MQ=255)

                                                          gATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCcgcg                                                                                      <  3:361567/151‑1 (MQ=255)

                                                            ttCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCgg                                                                                     >  2:70279/1‑150 (MQ=255)

                                                                ctcGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGtt                                                                                   <  3:1365199/148‑1 (MQ=255)

                                                                 tcGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCg                                                                               >  3:31396/1‑151 (MQ=255)

                                                                  cGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGt                                                                              <  2:142573/151‑1 (MQ=255)

                                                                   gCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTg                                                                             <  2:19886/151‑1 (MQ=255)

                                                                     tttCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTgg                                                                            >  3:268026/1‑150 (MQ=255)

                                                                          ctGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAAc                                                                         <  2:31692/148‑1 (MQ=255)

                                                                          ctGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGt                                                                      >  3:386925/1‑151 (MQ=255)

                                                                          ctGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGt                                                                      >  3:1071787/1‑151 (MQ=255)

                                                                          ctGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGt                                                                      >  3:312147/1‑151 (MQ=255)

                                                                               tAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAAt                                                                 >  2:217219/1‑151 (MQ=255)

                                                                                      tttCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCg                                                           >  1:322336/1‑150 (MQ=255)

                                                                                        tCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCgat                                                         <  1:70125/150‑1 (MQ=255)

                                                                                          cGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCgatgat                                                      >  3:562732/1‑151 (MQ=255)

                                                                                           gATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGatgatg                                                     >  2:235447/1‑151 (MQ=255)

                                                                                              tctcGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCgatga                                                       <  3:1363953/146‑1 (MQ=255)

                                                                                              tctcGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCgatg                                                        >  2:1363884/1‑145 (MQ=255)

                                                                                              tctcGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGa                                                   <  2:47928/150‑1 (MQ=255)

                                                                                               ctcGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGTGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGAtt                                                 >  3:234968/1‑151 (MQ=255)

                                                                                                tcGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGAttt                                                >  4:469531/1‑151 (MQ=255)

                                                                                                 cGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTg                                               >  1:312802/1‑151 (MQ=255)

                                                                                                 cGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTg                                               >  2:150609/1‑151 (MQ=255)

                                                                                                   aCGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTggg                                             >  2:419910/1‑151 (MQ=255)

                                                                                                    cGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGt                                            <  3:657052/151‑1 (MQ=255)

                                                                                                       tAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCg                                         >  3:1174794/1‑151 (MQ=255)

                                                                                                       tAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCg                                         >  1:267517/1‑151 (MQ=255)

                                                                                                         aCGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCgat                                       <  3:1316610/151‑1 (MQ=255)

                                                                                                         aCGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCgat                                       <  3:70289/151‑1 (MQ=255)

                                                                                                          cGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCgatg                                      <  1:312139/151‑1 (MQ=255)

                                                                                                          cGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCgatg                                      <  2:282856/151‑1 (MQ=255)

                                                                                                           gTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCgatga                                     <  3:999267/151‑1 (MQ=255)

                                                                                                              aaaCGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGAttt                                  <  3:500098/151‑1 (MQ=255)

                                                                                                                 cgcgTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTa                               <  3:290627/151‑1 (MQ=255)

                                                                                                                   cgTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTAcg                             >  2:5476/1‑151 (MQ=255)

                                                                                                                         ggCGCGCTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCg                       <  1:192277/151‑1 (MQ=255)

                                                                                                                          gcgcgcTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGa                      <  2:858369/151‑1 (MQ=255)

                                                                                                                          gcgcgcTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGa                      <  4:20321/151‑1 (MQ=255)

                                                                                                                           cgcgcTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGaa                     <  2:866100/151‑1 (MQ=255)

                                                                                                                            gcgcTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGaaa                    <  4:367855/151‑1 (MQ=255)

                                                                                                                             cgcTGCTATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAAc                   <  2:1258151/151‑1 (MQ=255)

                                                                                                                              gctgctATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAAcc                  <  3:1077087/151‑1 (MQ=255)

                                                                                                                               ctgctATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCg                 >  4:284298/1‑151 (MQ=255)

                                                                                                                                 gctATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCTGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGa                <  3:1372480/150‑1 (MQ=255)

                                                                                                                                 gctATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGaa               <  1:435549/151‑1 (MQ=255)

                                                                                                                                  ctATGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGTGTTGGGTGCGATGATTTGTACGCGCTCGAAACCGaa               >  3:349385/1‑150 (MQ=255)

                                                                                                                                    aTGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAAt             <  2:318661/150‑1 (MQ=255)

                                                                                                                                    aTGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATg            >  3:850007/1‑151 (MQ=255)

                                                                                                                                     tGCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGt           >  2:458604/1‑151 (MQ=255)

                                                                                                                                      gCTGTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGtt          >  2:588159/1‑151 (MQ=255)

                                                                                                                                         gTAAGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGTTc         <  3:975715/149‑1 (MQ=255)

                                                                                                                                            aGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGTTCGctct    <  2:1349531/151‑1 (MQ=255)

                                                                                                                                            aGCGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGTTCGctct    >  2:599749/1‑151 (MQ=255)

                                                                                                                                              cGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGTTCGCTCTGt  >  2:72699/1‑151 (MQ=255)

                                                                                                                                              cGGCTGACCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGTTCGCTCTGt  <  1:459897/151‑1 (MQ=255)

CGGAAACCTTTGAGGAAGTTGCAGCACTGCCGGGCGTCGGGCGTTCCACCGCAGGCGCGATTCTCTCGCTTTCTCTGGGTAAGCACTTTCCGATTCTCGACGGTAACGTCAAACGCGTGCTGGCGCGCTGCTATGCTGTAAGCGGCTGGCCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGTTCGCTCTGT  >  NC_000913/3103326‑3103618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`