Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 284,450 A→G E417G (GAG→GGG)  yagF → CP4‑6 prophage; dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913284,4500AG100.0% 446.4 / NA 121E417G (GAG→GGG) yagFCP4‑6 prophage; dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (55/66);  total (55/66)

Only 100 of 121 total aligned reads displayed.

GATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCG  >  NC_000913/284304‑284596
                                                                                                                                                  |                                                                                                                                                  
gATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCg                                                                                                                                                <  1:370639/151‑1 (MQ=255)
      ccACCTGCGCGACCTCGGCGTGGTGCATCTGGACGCCATGACCGTGGCCGGCCAGAGGGTGGGCGAGAGCCTTGAATGGTGGAAGGCGTCCGGGCGCCGGGCGCGCTTCAGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGAc                                                                                                                                          <  4:223710/151‑1 (MQ=255)
           tGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGAt                                                                                                                                     >  4:85027/1‑151 (MQ=255)
           tGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGAt                                                                                                                                     >  3:140370/1‑151 (MQ=255)
            gcgcGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGa                                                                                                                                      <  2:55791/149‑1 (MQ=255)
            gcgcGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATc                                                                                                                                    >  1:69875/1‑151 (MQ=255)
              gcgACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCt                                                                                                                                  <  4:171813/151‑1 (MQ=255)
                gACCTCGGCCCGCTGCATCTGGACGCCATGACCGTGACCGGCCAGGCGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGTAGGACGGCGTAGGGCCGGATGACGTGATcc                                                                                                                                   <  3:368184/148‑1 (MQ=255)
                   cTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTgccgc                                                                                                                             >  3:469245/1‑151 (MQ=255)
                     cGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGccgccg                                                                                                                           <  2:275931/151‑1 (MQ=255)
                      ggCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCgg                                                                                                                          >  2:1013166/1‑151 (MQ=255)
                       gCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGga                                                                                                                         <  3:769083/151‑1 (MQ=255)
                        ccTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGgag                                                                                                                        <  2:903196/151‑1 (MQ=255)
                        ccTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGgag                                                                                                                        <  1:206994/151‑1 (MQ=255)
                         ctgctgCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCgg                                                                                                                          >  3:737520/1‑148 (MQ=255)
                         ctgctgCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCgg                                                                                                                          >  3:915909/1‑148 (MQ=255)
                           gctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAg                                                                                                                     <  2:1170529/151‑1 (MQ=255)
                            ctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAgg                                                                                                                    >  3:1318741/1‑151 (MQ=255)
                            ctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAgg                                                                                                                    >  4:243732/1‑151 (MQ=255)
                            ctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAgg                                                                                                                    >  4:451783/1‑151 (MQ=255)
                               cATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaa                                                                                                                 <  3:317663/151‑1 (MQ=255)
                               cATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGGCCGGGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaa                                                                                                                 <  3:1375971/151‑1 (MQ=255)
                                aTCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaa                                                                                                                 >  2:1106493/1‑150 (MQ=255)
                                 tCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaaa                                                                                                                >  2:1009202/1‑150 (MQ=255)
                                    ggACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCaaa                                                                                                                <  3:1092425/147‑1 (MQ=255)
                                    ggACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCg                                                                                                            >  3:138579/1‑151 (MQ=255)
                                       cGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaaa                                                                                                         <  2:74976/151‑1 (MQ=255)
                                       cGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaa                                                                                                          >  2:583562/1‑150 (MQ=255)
                                        gCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGaaa                                                                                                         <  2:1065963/150‑1 (MQ=255)
                                          cATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAggg                                                                                                      <  3:898945/151‑1 (MQ=255)
                                          cATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAggg                                                                                                      >  2:747895/1‑151 (MQ=255)
                                          cATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAggg                                                                                                      <  3:1048871/151‑1 (MQ=255)
                                           aTGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGc                                                                                                     >  4:109540/1‑151 (MQ=255)
                                             gaccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCt                                                                                                    <  2:1148943/150‑1 (MQ=255)
                                              accgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTg                                                                                                   >  3:1104895/1‑150 (MQ=255)
                                               ccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGAc                                                                                                 <  2:956195/151‑1 (MQ=255)
                                                 gtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCt                                                                                               <  2:1244241/151‑1 (MQ=255)
                                                   gaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTc                                                                                              <  1:6937/150‑1 (MQ=255)
                                                   gaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCg                                                                                             >  1:292219/1‑151 (MQ=255)
                                                     ccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGAc                                                                                           <  3:843200/151‑1 (MQ=255)
                                                      tggCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACg                                                                                          >  3:780813/2‑151 (MQ=255)
                                                      cgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACg                                                                                          <  2:1043582/151‑1 (MQ=255)
                                                       ggCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACgg                                                                                         >  2:529139/1‑151 (MQ=255)
                                                          cAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCt                                                                                      <  2:1318670/151‑1 (MQ=255)
                                                           aGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTg                                                                                     <  3:397893/151‑1 (MQ=255)
                                                                gTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTcc                                                                                <  2:737488/151‑1 (MQ=255)
                                                                   ggCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGa                                                                             <  3:617463/151‑1 (MQ=255)
                                                                     cGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACg                                                                           >  2:543184/1‑151 (MQ=255)
                                                                     cGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACg                                                                           >  2:506282/1‑151 (MQ=255)
                                                                      gagaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACgg                                                                          <  2:969190/151‑1 (MQ=255)
                                                                       agaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACggg                                                                         <  3:730706/151‑1 (MQ=255)
                                                                        gaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACggg                                                                         <  3:516757/150‑1 (MQ=255)
                                                                        gaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGc                                                                        <  1:312086/151‑1 (MQ=255)
                                                                        gaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGc                                                                        >  2:1130301/1‑151 (MQ=255)
                                                                            cTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAAc                                                                     >  2:134022/1‑150 (MQ=255)
                                                                             ttGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACa                                                                    >  2:88466/1‑150 (MQ=255)
                                                                              tGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACa                                                                    <  2:960197/149‑1 (MQ=255)
                                                                              tGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACAt                                                                   <  3:872255/150‑1 (MQ=255)
                                                                               gAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATc                                                                  <  4:480712/150‑1 (MQ=255)
                                                                                aaTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGc                                                                <  3:401389/151‑1 (MQ=255)
                                                                                  tggtggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTc                                                              >  2:1157737/1‑151 (MQ=255)
                                                                                  tggtggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGGGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCt                                                               >  2:1076261/1‑150 (MQ=255)
                                                                                    gtggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCg                                                            >  1:236541/1‑151 (MQ=255)
                                                                                          ggCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAgg                                                       >  3:517614/1‑150 (MQ=255)
                                                                                            cGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTc                                                    <  2:202228/151‑1 (MQ=255)
                                                                                             gTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCg                                                   >  2:678498/1‑151 (MQ=255)
                                                                                             gTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCg                                                   >  3:309932/1‑151 (MQ=255)
                                                                                             gTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCg                                                   <  2:481346/151‑1 (MQ=255)
                                                                                                 gAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGa                                               <  1:109541/151‑1 (MQ=255)
                                                                                                  aGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGAt                                              >  2:1159112/1‑151 (MQ=255)
                                                                                                    cgcCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATc                                             >  4:113908/1‑150 (MQ=255)
                                                                                                    cgcCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCa                                            <  2:1104843/151‑1 (MQ=255)
                                                                                                     gcCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCTTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCa                                            >  2:1284561/1‑150 (MQ=255)
                                                                                                      ccGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCaa                                           <  2:1177245/150‑1 (MQ=255)
                                                                                                        gggCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAgg                                         <  2:1321608/150‑1 (MQ=255)
                                                                                                        gggCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGc                                        >  2:1364132/1‑151 (MQ=255)
                                                                                                          gcgcgcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGCAGGTTCGGTGATCAAGGcc                                       >  3:1015564/1‑150 (MQ=255)
                                                                                                           cgcgcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCAc                                     >  4:245747/1‑151 (MQ=255)
                                                                                                            gcgcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACg                                    >  2:1363786/1‑151 (MQ=255)
                                                                                                               cTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCg                                 >  3:538001/1‑151 (MQ=255)
                                                                                                                 tCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCg                                 <  1:161866/149‑1 (MQ=255)
                                                                                                                 tCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGAt                               >  3:562153/1‑151 (MQ=255)
                                                                                                                  ccGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATc                              <  3:221777/151‑1 (MQ=255)
                                                                                                                    gCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCg                             >  2:658199/1‑150 (MQ=255)
                                                                                                                      cAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGAcc                          <  1:391091/151‑1 (MQ=255)
                                                                                                                       aGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGAccc                         >  1:112209/1‑151 (MQ=255)
                                                                                                                              gcgcGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggtg                  <  2:964371/151‑1 (MQ=255)
                                                                                                                              gcgcGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggt                   <  4:27971/150‑1 (MQ=255)
                                                                                                                              gcgcGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCgg                    <  1:303214/149‑1 (MQ=255)
                                                                                                                               cgcgAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggtgg                 <  2:860769/151‑1 (MQ=255)
                                                                                                                                gcgAGCAGGACGGCGTAGGGCCGGAGGGCGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGTCGGTCTGCTTCCCGACGGGCAACAGCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggtggt                <  3:781167/151‑1 (MQ=255)
                                                                                                                                 cgAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGgtggtg               >  2:908150/1‑151 (MQ=255)
                                                                                                                                  gAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGtggtgg              >  4:278291/1‑151 (MQ=255)
                                                                                                                                     cAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCg           <  2:138562/151‑1 (MQ=255)
                                                                                                                                     cAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCg           <  2:140354/151‑1 (MQ=255)
                                                                                                                                       ggACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGaa         <  3:529173/151‑1 (MQ=255)
                                                                                                                                       ggACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGaa         <  2:780778/151‑1 (MQ=255)
                                                                                                                                          cGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGAt      <  4:67003/151‑1 (MQ=255)
                                                                                                                                             cGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGc   <  2:469210/151‑1 (MQ=255)
                                                                                                                                              gTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCg  >  3:1006191/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCG  >  NC_000913/284304‑284596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑