Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,128,355 T→C G28G (GGT→GGC glcC → glycolate‑inducible glc operon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,128,3550TC100.0% 447.7 / NA 122G28G (GGT→GGCglcCglycolate‑inducible glc operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/68);  total (54/68)

Only 100 of 124 total aligned reads displayed.

GTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGTCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCGTGTCGCACGGCTTAATCGGGTG  >  NC_000913/3128212‑3128502
                                                                                                                                               |                                                                                                                                                   
gtgCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCg                                                                                                                                               <  3:357508/150‑1 (MQ=255)
    gtgtTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTcc                                                                                                                                          <  2:362536/151‑1 (MQ=255)
     tgctttGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGACCTATTTGCGAAGTGGTTGCAGAGAGTATCGCACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTccc                                                                                                                                         >  4:313715/4‑151 (MQ=255)
       ttttGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTc                                                                                                                                       >  2:858732/1‑151 (MQ=255)
        tttGCGAGTTAAGAACAGAAAAATTGGTCCTTCCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTTCTGAAGGTCGGCCAGCCGCTTCCCTc                                                                                                                                       <  3:1109252/150‑1 (MQ=255)
         ttGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCgg                                                                                                                                     >  4:309628/1‑151 (MQ=255)
          tGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCgg                                                                                                                                     <  3:106325/150‑1 (MQ=255)
          tGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCgg                                                                                                                                     >  3:496046/1‑150 (MQ=255)
           gCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGa                                                                                                                                    <  4:46632/150‑1 (MQ=255)
           gCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGa                                                                                                                                    >  2:1392097/1‑150 (MQ=255)
               gTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTc                                                                                                                               <  3:152015/151‑1 (MQ=255)
                 tAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCg                                                                                                                              >  1:89568/1‑150 (MQ=255)
                  aaGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGAc                                                                                                                            >  3:772727/1‑151 (MQ=255)
                  aaGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGAc                                                                                                                            <  3:1403598/151‑1 (MQ=255)
                    gAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACtg                                                                                                                          <  2:224946/151‑1 (MQ=255)
                      aCAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACtgtg                                                                                                                        >  2:60454/1‑151 (MQ=255)
                      aCAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACtgtg                                                                                                                        >  4:123235/1‑151 (MQ=255)
                      aCAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACtgtg                                                                                                                        <  1:364495/151‑1 (MQ=255)
                            aaaTTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGaa                                                                                                                    >  2:989910/1‑149 (MQ=255)
                                tGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCt                                                                                                               <  3:672651/150‑1 (MQ=255)
                                 ggTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAATGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTc                                                                                                              <  3:618745/150‑1 (MQ=255)
                                    ccTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAACGCTCGGCt                                                                                                          <  3:858769/151‑1 (MQ=255)
                                        ccTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGACCGTCGACTGTGTGAAAAGCTCGGCTtc                                                                                                        <  3:232071/149‑1 (MQ=255)
                                        ccTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTtctc                                                                                                      >  3:1136488/1‑151 (MQ=255)
                                          tgtgCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCa                                                                                                     >  2:756538/1‑150 (MQ=255)
                                           gtgCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCAc                                                                                                    <  3:967622/150‑1 (MQ=255)
                                                cGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCg                                                                                              >  4:222267/1‑151 (MQ=255)
                                                cGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCg                                                                                              <  3:1195287/151‑1 (MQ=255)
                                                 gAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGc                                                                                             >  2:695118/1‑151 (MQ=255)
                                                 gAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGc                                                                                             >  3:962792/1‑151 (MQ=255)
                                                  aGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGc                                                                                             >  4:333689/1‑150 (MQ=255)
                                                    gTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACt                                                                                          <  2:374115/151‑1 (MQ=255)
                                                      ccGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGc                                                                                        >  4:193766/1‑151 (MQ=255)
                                                         ggAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTg                                                                                     >  2:1067558/1‑151 (MQ=255)
                                                         ggAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTg                                                                                     <  3:3931/151‑1 (MQ=255)
                                                            aTGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAg                                                                                  <  3:844673/151‑1 (MQ=255)
                                                            aTGAAAGATGAACGTCGCCCTATTTGCGAAGCGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGATCGGCTTCTCACGCTCCGCACTGCGTGa                                                                                    >  2:983941/1‑149 (MQ=255)
                                                              gAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAggg                                                                                <  2:125836/151‑1 (MQ=255)
                                                              gAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAgg                                                                                 <  1:44321/150‑1 (MQ=255)
                                                              gAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAgg                                                                                 <  3:486151/150‑1 (MQ=255)
                                                                aaGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCt                                                                              >  1:237271/1‑151 (MQ=255)
                                                                aaGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCt                                                                              <  2:962755/151‑1 (MQ=255)
                                                                  gATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGa                                                                            <  4:270936/151‑1 (MQ=255)
                                                                    tGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGAcc                                                                          >  1:269471/1‑151 (MQ=255)
                                                                     gAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGAcc                                                                          <  2:553829/150‑1 (MQ=255)
                                                                     gAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGAcc                                                                          <  1:333692/150‑1 (MQ=255)
                                                                     gAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGAcc                                                                          <  2:1185493/150‑1 (MQ=255)
                                                                      aaCGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGt                                                                        >  3:974168/1‑151 (MQ=255)
                                                                      aaCGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGt                                                                        <  2:1009041/151‑1 (MQ=255)
                                                                      aaCGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGt                                                                        <  2:208060/151‑1 (MQ=255)
                                                                       aCGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGtg                                                                       <  3:206698/151‑1 (MQ=255)
                                                                       aCGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGtg                                                                       <  1:273654/151‑1 (MQ=255)
                                                                        cGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGGCGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGtg                                                                       <  3:1399342/150‑1 (MQ=255)
                                                                          tCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGtgctg                                                                    >  2:651918/1‑151 (MQ=255)
                                                                           cGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGtgctg                                                                    >  2:399771/1‑150 (MQ=255)
                                                                            gCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGGTAATTATCGACGGCGTACTGAAGGACGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTATCACGCTCCGCACTGCGTGAATGGCTGACCGTGCTgcg                                                                  >  4:432437/1‑151 (MQ=255)
                                                                               cTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTgcgcgg                                                               >  2:1216775/1‑151 (MQ=255)
                                                                                tATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTgcgcggg                                                              >  1:127167/1‑151 (MQ=255)
                                                                                 aTTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTgcgcgggc                                                             >  3:1403698/1‑151 (MQ=255)
                                                                                 aTTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTgcgcgggc                                                             >  3:483911/1‑151 (MQ=255)
                                                                                   ttGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGATGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTgcgcgggcg                                                            >  4:39026/1‑150 (MQ=255)
                                                                                   ttGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTgcgcgggcgc                                                           >  3:650725/1‑151 (MQ=255)
                                                                                    tGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTgcgcgggcgcg                                                          >  3:1171755/1‑151 (MQ=255)
                                                                                    tGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTgcgcgggcgcg                                                          >  2:514938/1‑151 (MQ=255)
                                                                                    tGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTgcgcgggcgcg                                                          >  3:593461/1‑151 (MQ=255)
                                                                                    tGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTgcgcgggcgc                                                           <  3:1392168/150‑1 (MQ=255)
                                                                                        aaGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGat                                                      >  2:377545/1‑151 (MQ=255)
                                                                                          gTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGatta                                                    <  2:1069111/151‑1 (MQ=255)
                                                                                            ggTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGattatt                                                  <  4:437020/151‑1 (MQ=255)
                                                                                            ggTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGattat                                                   <  2:1171697/150‑1 (MQ=255)
                                                                                            ggTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGattat                                                   <  3:208443/150‑1 (MQ=255)
                                                                                            ggTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGattat                                                   <  3:1268369/150‑1 (MQ=255)
                                                                                             gTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTg                                                 <  3:1183345/151‑1 (MQ=255)
                                                                                              ttGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGTGGGCGCGGGATTATTg                                                 >  3:243976/1‑150 (MQ=255)
                                                                                               tGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGa                                                <  3:332242/150‑1 (MQ=255)
                                                                                                  agagagTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACg                                            <  4:477679/151‑1 (MQ=255)
                                                                                                       gTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCa                                       <  3:839294/151‑1 (MQ=255)
                                                                                                         aTCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAgg                                     <  2:772692/151‑1 (MQ=255)
                                                                                                         aTCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAgg                                     <  2:1362582/151‑1 (MQ=255)
                                                                                                            gAACGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTc                                  <  1:397437/151‑1 (MQ=255)
                                                                                                             aaCGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTcg                                 <  2:1152145/151‑1 (MQ=255)
                                                                                                               cGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTcgcg                               >  1:402534/1‑151 (MQ=255)
                                                                                                               cGGTTAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTcgcg                               <  4:453322/151‑1 (MQ=255)
                                                                                                                  ttAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGAtt                            >  3:610185/1‑151 (MQ=255)
                                                                                                                   tAATTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGAtt                            <  3:473266/150‑1 (MQ=255)
                                                                                                                    aaTTATCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATtc                           <  3:395254/150‑1 (MQ=255)
                                                                                                                     aTTATCGGCGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGCCTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATtctc                         <  3:204686/151‑1 (MQ=255)
                                                                                                                        aTCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCgtg                      <  1:443752/151‑1 (MQ=255)
                                                                                                                        aTCGACGGCGTACTGAAGGGCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCgt                       >  3:1036511/1‑150 (MQ=255)
                                                                                                                         tCGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCgtgt                     >  4:317979/1‑151 (MQ=255)
                                                                                                                          cGACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCGTGTc                    >  3:371878/1‑151 (MQ=255)
                                                                                                                           gACGGCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCGTGTCg                   <  4:413192/151‑1 (MQ=255)
                                                                                                                              ggCGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCGTGTCGCAc                >  3:906562/1‑151 (MQ=255)
                                                                                                                                cGTACTGAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCGTGTCGCACgg              <  2:1136433/151‑1 (MQ=255)
                                                                                                                                      gAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCGTGTCGCACGGCTTaa         <  3:326610/150‑1 (MQ=255)
                                                                                                                                      gAAGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCGTGTCGCACGGCTTAAt        >  2:794487/1‑151 (MQ=255)
                                                                                                                                        aGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCGTGTCGCACGGCTTAATCg      >  1:289965/1‑151 (MQ=255)
                                                                                                                                        aGGTCGGCCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCGTGTCGCACGGCTTAATCg      >  3:39895/1‑151 (MQ=255)
                                                                                                                                          gtcggccAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCGTGTCGCACGGCTTAATCggg    <  4:474377/151‑1 (MQ=255)
                                                                                                                                            cggccAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCGTGTCGCACGGCTTAATCGGGTg  <  2:483877/151‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAATGAAAGATGAACGTCGCCCTATTTGCGAAGTGGTTGCAGAGAGTATCGAACGGTTAATTATCGACGGCGTACTGAAGGTCGGTCAGCCGCTTCCCTCGGAACGTCGACTGTGTGAAAAGCTCGGCTTCTCACGCTCCGCACTGCGTGAAGGGCTGACCGTGCTGCGCGGGCGCGGGATTATTGAAACGGCGCAGGGTCGCGATTCTCGTGTCGCACGGCTTAATCGGGTG  >  NC_000913/3128212‑3128502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: