Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,356,384 T→C S554P (TCG→CCG)  gltB → glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,356,3840TC100.0% 463.3 / NA 120S554P (TCG→CCG) gltBglutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/69);  total (51/69)

Only 100 of 121 total aligned reads displayed.

CTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGAAAA  >  NC_000913/3356239‑3356529
                                                                                                                                                 |                                                                                                                                                 
ctcccGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCg                                                                                                                                                 <  4:477626/145‑1 (MQ=255)
ctTCCGCCAGCAGTTTGCCCAGGTGCCTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGcc                                                                                                                                               <  4:192893/150‑1 (MQ=255)
 ttCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAAccgccg                                                                                                                                              >  2:101678/1‑150 (MQ=255)
    cGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTc                                                                                                                                          >  1:37660/1‑151 (MQ=255)
         gcatTTTGCCGAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCg                                                                                                                                                 <  4:128526/139‑1 (MQ=255)
             tttGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACt                                                                                                                                 >  2:132404/1‑151 (MQ=255)
             ttggCCCGGGTGATTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCCCCAGTATCGGTCGTAAAATGAACTTCTTTTGCGAAGCAGAGGGCCAGGCCCACGGTTTAAGCTTTAAACCGCCGATTCTGct                                                                                                                                      <  1:132193/143‑1 (MQ=255)
             ttgccccGGGTAACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGctc                                                                                                                                     <  4:141897/143‑1 (MQ=255)
                 cccGGGTGACAACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCCCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTcc                                                                                                                               <  2:921505/149‑1 (MQ=255)
                   cAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGAtt                                                                                                                           >  3:1372783/1‑151 (MQ=255)
                    aGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGAttt                                                                                                                          >  2:293507/1‑151 (MQ=255)
                     gttgccaaccccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTc                                                                                                                         <  2:460275/142‑1 (MQ=255)
                     ggTGACAACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCGGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGAtt                                                                                                                           <  4:228872/149‑1 (MQ=255)
                       tGACAACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGAttt                                                                                                                          <  4:129925/148‑1 (MQ=255)
                            accccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAg                                                                                                                   <  1:32463/148‑1 (MQ=255)
                              cccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCTTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCa                                                                                                                        <  2:546391/143‑1 (MQ=255)
                               ccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCa                                                                                                               <  2:386155/151‑1 (MQ=255)
                               ccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGAGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCa                                                                                                               <  3:844203/151‑1 (MQ=255)
                                cGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCac                                                                                                              <  2:1103738/151‑1 (MQ=255)
                                  ccacgcGACCCGCTGCGTGAAGCGATTTTTATTTCGCTCGCCACCATTATCGGTCTGGAAATGAACTTTTTTTGGAAAGCAGAGGGCAAGGCGCCCCTTTAAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCacg                                                                                                             <  3:900456/145‑1 (MQ=255)
                                  ccAATCGCCCCGCTGCGTGAAGCGAATGTTATGCCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTTTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCac                                                                                                              <  1:415471/149‑1 (MQ=255)
                                  ccAATCGACCCGCTGCGTGAAGCGCATTTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCacga                                                                                                            <  3:56444/151‑1 (MQ=255)
                                  ccAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCacg                                                                                                             <  2:1333131/150‑1 (MQ=255)
                                   cAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCacgac                                                                                                           >  3:1191830/1‑151 (MQ=255)
                                    aagcGACCCGCTGCGTGAAGCGAAATTTAAGTCTCTCGCCACCAGTATCGGTCTTAAAATGAACGCCTTTTGGGATGCTGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCacg                                                                                                             <  2:1099915/145‑1 (MQ=255)
                                    aaTCGCCCCGCTGCGTGAAGCTCATGTTATGTCCCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCacgacg                                                                                                          <  1:102409/151‑1 (MQ=255)
                                       cGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATg                                                                                                       >  1:147545/1‑151 (MQ=255)
                                        gACCCGCTGGGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACTTCTTTTGGAAAGCAGAGGGCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGAt                                                                                                        <  3:1020213/149‑1 (MQ=255)
                                         aCCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGa                                                                                                      >  2:1388636/1‑150 (MQ=255)
                                          cccGCGGCATAAAGCCCGTGTTAGGTCCCTCCCCACCACGTTCGGTCGTGAATTGACCTTTTTTTGTGAAGCTGAGGCCCATGCCCTCCTTTTAAGCTTGAAACCGCCGATTCTGCTCTACGCCGATTTCAAACAGCTCAcgacgac                                                                                                        <  4:16671/147‑2 (MQ=255)
                                            cGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGaa                                                                                                     >  3:61085/1‑148 (MQ=255)
                                            cGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAaga                                                                                                  <  1:371575/151‑1 (MQ=255)
                                             gCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGCCCAGGCGCACCTTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAaga                                                                                                  <  3:1025077/150‑1 (MQ=255)
                                               tGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAgagg                                                                                                >  2:419445/1‑150 (MQ=255)
                                                gCGTGAGGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAAGGACCGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACGCCGATTTCAAACAGCTCACGACGATg                                                                                                       <  4:391280/142‑1 (MQ=255)
                                                  gtaaaGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCAAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCa                                                                                            <  3:16800/148‑1 (MQ=255)
                                                  gTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGc                                                                                             <  1:281482/150‑1 (MQ=255)
                                                      aGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTAc                                                                                        >  1:328975/1‑151 (MQ=255)
                                                         gcATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACcgc                                                                                     >  2:525445/1‑151 (MQ=255)
                                                          cATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTGGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACcgc                                                                                     <  2:372884/150‑1 (MQ=255)
                                                             tttatttcccgccccaccgGTATGGGTAGGGAAAGTACCGTCTTTTGAGAAGCAGGGGGCGAGGCGCGTCTTTTAAGGTTGAAGCCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCgcgc                                                                                   <  3:770098/136‑1 (MQ=255)
                                                             gTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCgcgc                                                                                   >  1:309163/1‑149 (MQ=255)
                                                                 tGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGAt                                                                               <  2:364139/149‑1 (MQ=255)
                                                                 tGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATa                                                                              <  2:187237/150‑1 (MQ=255)
                                                                 tGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATAc                                                                             >  2:1009543/1‑151 (MQ=255)
                                                                 tGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATAc                                                                             >  2:871222/1‑151 (MQ=255)
                                                                 tGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTAAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATa                                                                              <  3:478716/150‑1 (MQ=255)
                                                                     gcacgcCACCGGTACTTGTCGAAAAATCAGCGTCTTTTGCGAAGCGGAGGGCCAGGCACACCGTTTAAGCTTAAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGc                                                                           <  3:1162849/146‑1 (MQ=255)
                                                                      ctcgcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTgg                                                                        >  3:66518/1‑151 (MQ=255)
                                                                      cccgcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTg                                                                         <  3:1076422/148‑1 (MQ=255)
                                                                       tcgcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTgg                                                                        >  1:151438/1‑150 (MQ=255)
                                                                        cgcCCCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGAAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGat                                                                      <  2:496847/151‑1 (MQ=255)
                                                                        cgcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGat                                                                      >  1:480904/1‑151 (MQ=255)
                                                                         gcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGat                                                                      >  3:505276/1‑150 (MQ=255)
                                                                          ccaccaGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGatat                                                                    <  2:127095/151‑1 (MQ=255)
                                                                             ccaGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATc                                                                   <  1:240324/149‑1 (MQ=255)
                                                                             ccaGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCAc                                                                 >  2:1180481/1‑151 (MQ=255)
                                                                               aGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCt                                                               >  2:665135/1‑151 (MQ=255)
                                                                                  aTCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTg                                                            >  3:533387/1‑151 (MQ=255)
                                                                                    cGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCttt                                                             <  2:467594/148‑1 (MQ=255)
                                                                                       tCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTc                                                       >  2:838653/1‑151 (MQ=255)
                                                                                        cGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCa                                                      <  1:415800/151‑1 (MQ=255)
                                                                                        cGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCa                                                      <  3:132419/151‑1 (MQ=255)
                                                                                         gTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCAc                                                     >  3:137932/1‑151 (MQ=255)
                                                                                           gAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACt                                                    >  3:554420/1‑150 (MQ=255)
                                                                                            aaaTGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTa                                                   >  1:450097/1‑150 (MQ=255)
                                                                                                 aaCGTCTTTGGCGAAGCAGAGGGCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAAc                                               <  3:761681/149‑1 (MQ=255)
                                                                                                   cGTCTTTTGCAAAGCAGAGGGCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCAc                                            <  3:515756/150‑1 (MQ=255)
                                                                                                    gTCTTTTGCGAAGCAGAGGGCCGGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACg                                           <  3:494246/150‑1 (MQ=255)
                                                                                                     tCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCAc                                            <  3:694191/148‑1 (MQ=255)
                                                                                                        tttGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCg                                       <  1:461615/150‑1 (MQ=255)
                                                                                                        tttGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTAAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCg                                       <  3:295551/150‑1 (MQ=255)
                                                                                                         ttGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGa                                      >  3:27070/1‑150 (MQ=255)
                                                                                                         ttGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGa                                      >  4:220972/1‑150 (MQ=255)
                                                                                                           gCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGc                                   >  2:1211368/1‑151 (MQ=255)
                                                                                                              aaGCAGAGGGCCAGGCGCACCTTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAg                                    <  3:366892/147‑1 (MQ=255)
                                                                                                              aaGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGAc                                >  3:857589/1‑151 (MQ=255)
                                                                                                                  agagGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTc                            >  3:1393393/1‑151 (MQ=255)
                                                                                                                   gagGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTc                            >  3:287259/1‑150 (MQ=255)
                                                                                                                     gggCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCa                           <  3:1288837/149‑1 (MQ=255)
                                                                                                                     gggCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCaa                          <  4:449216/150‑1 (MQ=255)
                                                                                                                      ggCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAag                        <  2:867111/151‑1 (MQ=255)
                                                                                                                          aGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCt                    >  3:554339/1‑151 (MQ=255)
                                                                                                                          aGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCt                    >  2:721948/1‑151 (MQ=255)
                                                                                                                            gcgcCCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCtgt                  <  3:101692/151‑1 (MQ=255)
                                                                                                                              gcACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGc                >  2:203935/1‑151 (MQ=255)
                                                                                                                               cACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCg               <  2:1191773/151‑1 (MQ=255)
                                                                                                                               cACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCg               <  2:481705/151‑1 (MQ=255)
                                                                                                                               cACCGTTTAAGCTTAAACCCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTCCCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGc                <  1:13116/150‑1 (MQ=255)
                                                                                                                                aCCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCg               >  2:630983/1‑150 (MQ=255)
                                                                                                                                 ccGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGc                <  4:473379/148‑1 (MQ=255)
                                                                                                                                  cGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGAc             >  2:114668/1‑150 (MQ=255)
                                                                                                                                   gTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACaa           <  3:355542/151‑1 (MQ=255)
                                                                                                                                     ttAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACaaa          <  3:1180536/150‑1 (MQ=255)
                                                                                                                                      tAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGc        >  2:1153010/1‑151 (MQ=255)
                                                                                                                                        aGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGcc       >  3:1364962/1‑150 (MQ=255)
                                                                                                                                           tttAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGaa    >  2:754116/1‑150 (MQ=255)
                                                                                                                                            taaaaCCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGaaa   <  1:366711/148‑1 (MQ=255)
                                                                                                                                              aaaCCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGaaaa  <  3:771618/149‑1 (MQ=255)
                                                                                                                                              aaaCCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGaaaa  <  2:1214960/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGAAAA  >  NC_000913/3356239‑3356529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑