BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,430,876	A→G	D180D (GAT→GAC)	tsaC ←	tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,430,876	0	A	G	100.0%	473.7 / NA	131	D180D (GAT→GAC)	tsaC	tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (68/63); total (68/63)

Only 100 of 131 total aligned reads displayed.

CAACACGCGCCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTA  >  NC_000913/3430728‑3431020

cAACACGCGCCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTc                                                                                                                                                <  2:1198753/151‑1 (MQ=255)

 aaCACGCGCCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTcg                                                                                                                                               >  2:185156/1‑151 (MQ=255)

   cacGCGCCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTcgcg                                                                                                                                             <  2:1258348/151‑1 (MQ=255)

    acGCGCCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCgg                                                                                                                                            <  2:246708/151‑1 (MQ=255)

     cgcgCCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCgg                                                                                                                                            >  3:11410/1‑150 (MQ=255)

      gcgcCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGAt                                                                                                                                          <  2:902768/151‑1 (MQ=255)

      gcgcCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGAt                                                                                                                                          <  2:1352661/151‑1 (MQ=255)

       cgcCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGAtt                                                                                                                                         <  3:1283568/151‑1 (MQ=255)

       cgcCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGAtt                                                                                                                                         <  1:347907/151‑1 (MQ=255)

       cgcCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGAtt                                                                                                                                         >  3:1365146/1‑151 (MQ=255)

        gcCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGAttt                                                                                                                                        >  2:353279/1‑151 (MQ=255)

        gcCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGAttt                                                                                                                                        <  3:462411/151‑1 (MQ=255)

         cccATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTc                                                                                                                                       <  3:1198937/151‑1 (MQ=255)

         cccATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTGCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTc                                                                                                                                       <  3:291563/151‑1 (MQ=255)

          ccATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCt                                                                                                                                      <  2:656637/151‑1 (MQ=255)

              aGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGaa                                                                                                                                   <  3:612744/150‑1 (MQ=255)

                ggATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAgg                                                                                                                                 >  3:1388317/1‑150 (MQ=255)

                    gTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCCCCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTa                                                                                                                            <  1:262499/151‑1 (MQ=255)

                     ttCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTa                                                                                                                            >  2:1249858/1‑150 (MQ=255)

                      tCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTaa                                                                                                                           <  2:252503/150‑1 (MQ=255)

                      tCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTaa                                                                                                                           <  3:987411/150‑1 (MQ=255)

                        aaTATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACg                                                                                                                        <  3:478190/151‑1 (MQ=255)

                         atatTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGc                                                                                                                       >  2:844912/1‑151 (MQ=255)

                         atatTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGc                                                                                                                       >  2:360077/1‑151 (MQ=255)

                         atatTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGc                                                                                                                       >  2:45329/1‑151 (MQ=255)

                          tatTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGcc                                                                                                                      >  2:1272394/1‑151 (MQ=255)

                          tatTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGcc                                                                                                                      >  3:682815/1‑151 (MQ=255)

                          tatTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGcc                                                                                                                      >  4:470200/1‑151 (MQ=255)

                            ttCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGcccc                                                                                                                    >  1:216492/1‑151 (MQ=255)

                              cAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGcccccc                                                                                                                  >  1:327805/1‑151 (MQ=255)

                                  tGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGtt                                                                                                              >  3:1383887/1‑151 (MQ=255)

                                  tGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGt                                                                                                               <  2:816224/150‑1 (MQ=255)

                                     tGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTc                                                                                                            >  4:357742/1‑150 (MQ=255)

                                       aGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCAcc                                                                                                         >  3:29098/1‑151 (MQ=255)

                                         cAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCAc                                                                                                          >  4:94227/1‑148 (MQ=255)

                                             ttGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGcac                                                                                                   >  4:18994/1‑151 (MQ=255)

                                             ttGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGcac                                                                                                   >  1:132353/1‑151 (MQ=255)

                                                cTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACaa                                                                                                 <  1:81107/150‑1 (MQ=255)

                                                  gatgaATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCg                                                                                              <  2:37079/151‑1 (MQ=255)

                                                   atgaatgaATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCgg                                                                                             >  3:585741/1‑151 (MQ=255)

                                                          aatgGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAAcgcc                                                                                      >  4:372247/1‑151 (MQ=255)

                                                          aatgGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAAcgcc                                                                                      <  3:360108/151‑1 (MQ=255)

                                                           atgGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAAcgccg                                                                                     >  2:1072075/1‑151 (MQ=255)

                                                           atgGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAAcgccg                                                                                     >  2:1180281/1‑151 (MQ=255)

                                                             ggCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCcgcg                                                                                   >  2:1402032/1‑151 (MQ=255)

                                                               cGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGcc                                                                                 >  2:468092/1‑151 (MQ=255)

                                                                 aTTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCaa                                                                               <  3:793364/151‑1 (MQ=255)

                                                                       tgtgGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATtgtg                                                                         >  2:759818/1‑151 (MQ=255)

                                                                        gtgGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATtgt                                                                          <  3:582726/149‑1 (MQ=255)

                                                                        gtgGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTgc                                                                        <  2:1388247/151‑1 (MQ=255)

                                                                         tgGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTgcg                                                                       >  3:585815/1‑151 (MQ=255)

                                                                            gCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGa                                                                    <  2:874591/151‑1 (MQ=255)

                                                                               aTCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGGACAACCGGGAACGCCGCGCCAAATTGTGCGCGaa                                                                   <  2:635002/149‑1 (MQ=255)

                                                                               aTCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGaa                                                                   <  3:658412/149‑1 (MQ=255)

                                                                               aTCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAAc                                                                  <  4:290152/150‑1 (MQ=255)

                                                                               aTCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACt                                                                 <  1:257249/151‑1 (MQ=255)

                                                                               aTCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACt                                                                 >  4:193281/1‑151 (MQ=255)

                                                                                 cGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTc                                                               >  1:477214/1‑151 (MQ=255)

                                                                                   gATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGt                                                             <  2:907143/151‑1 (MQ=255)

                                                                                     ttACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCt                                                           >  3:71990/1‑151 (MQ=255)

                                                                                      tACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCt                                                           <  2:11407/150‑1 (MQ=255)

                                                                                      tACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCt                                                           <  2:1083195/150‑1 (MQ=255)

                                                                                       aCCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTAc                                                         >  3:476556/1‑151 (MQ=255)

                                                                                          aaaaaCAGCATAGGATTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCt                                                           >  2:1063446/1‑146 (MQ=255)

                                                                                           aaaaCAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGtt                                                     <  4:297213/151‑1 (MQ=255)

                                                                                            aaaCAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTc                                                    <  4:266984/151‑1 (MQ=255)

                                                                                            aaaCAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTc                                                    >  4:356143/1‑151 (MQ=255)

                                                                                                aGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGAc                                                 >  3:785754/1‑150 (MQ=255)

                                                                                                   aTAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAgg                                             >  3:693723/1‑151 (MQ=255)

                                                                                                     aGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTg                                           <  2:585371/151‑1 (MQ=255)

                                                                                                           ccATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATc                                     >  2:118718/1‑151 (MQ=255)

                                                                                                            cATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATcc                                    <  3:613991/151‑1 (MQ=255)

                                                                                                            cATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATcc                                    <  2:682791/151‑1 (MQ=255)

                                                                                                            cATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATcc                                    <  1:7924/151‑1 (MQ=255)

                                                                                                             aTTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCa                                   <  3:93369/151‑1 (MQ=255)

                                                                                                                aTGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACt                                 <  2:693698/150‑1 (MQ=255)

                                                                                                                    tACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAg                             <  2:476521/150‑1 (MQ=255)

                                                                                                                    tACCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAg                             <  3:439253/150‑1 (MQ=255)

                                                                                                                     aCCCCTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAg                             <  3:45340/149‑1 (MQ=255)

                                                                                                                      ccccTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTg                          <  3:323829/151‑1 (MQ=255)

                                                                                                                      ccccTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTg                          >  2:871048/1‑151 (MQ=255)

                                                                                                                         cTGTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGc                        >  3:1015079/1‑150 (MQ=255)

                                                                                                                           gTCGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCa                       <  3:576961/149‑1 (MQ=255)

                                                                                                                             cGACACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTgg                   >  3:368280/1‑151 (MQ=255)

                                                                                                                             cGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTgg                   >  4:447092/1‑151 (MQ=255)

                                                                                                                             cGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTgg                   >  1:344501/1‑151 (MQ=255)

                                                                                                                             cGAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTg                    <  4:19487/150‑1 (MQ=255)

                                                                                                                              gAAACAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGt                  >  2:982722/1‑151 (MQ=255)

                                                                                                                               aaaCAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTa                 <  3:1272460/151‑1 (MQ=255)

                                                                                                                                aaCAGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGt                  >  3:930524/1‑149 (MQ=255)

                                                                                                                                   aGTTCACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTTTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGaa              >  4:1093/1‑150 (MQ=255)

                                                                                                                                       cACCCGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAAcc           >  3:520374/1‑149 (MQ=255)

                                                                                                                                         cccGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAgg        <  3:355695/150‑2 (MQ=255)

                                                                                                                                         cccGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCg       >  3:618004/1‑151 (MQ=255)

                                                                                                                                           cGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGt     >  1:254905/1‑151 (MQ=255)

                                                                                                                                           cGTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGt     >  1:143434/1‑151 (MQ=255)

                                                                                                                                            gTCAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGtt    <  2:845977/151‑1 (MQ=255)

                                                                                                                                              cAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGttt   <  2:585710/150‑1 (MQ=255)

                                                                                                                                              cAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTa  <  2:384841/151‑1 (MQ=255)

                                                                                                                                              cAGGGCGTCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTa  <  1:212356/151‑1 (MQ=255)

CAACACGCGCCCATAGGGATGTTCAATATTCAGTTGCTGAGCAAATTGCTGATGAATGAATGGCGATTTGCTGTGGGCTATCGGATTACCAAAAACAGCATAGGTTTCCATTATGTTACCCCTGTCGAAACAGTTCACCCGTCAGGGCATCGCGGATTTCTGAAGGATTTAAACGCCCCCCCGTTTCACCAGGCACAACCGGGAACGCCGCGCCAAATTGTGCGCGAACTTCGTCTACTGTTCGACAAGGTGGCAATCCACTCAAGTTGGCACTGGTAGAAACCAGCGGTTTA  >  NC_000913/3430728‑3431020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`