Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,439,982 A→G S6S (AGT→AGC)  rplQ ← 50S ribosomal subunit protein L17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,439,9820AG99.1% 411.6 / NA 108S6S (AGT→AGC) rplQ50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (50/57);  minor base T (1/0);  total (51/57)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

GCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCT  >  NC_000913/3439836‑3440125
                                                                                                                                                  |                                                                                                                                               
gCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCAGGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGctct                                                                                                                                             <  2:655900/151‑1 (MQ=255)
 cAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCtt                                                                                                                                            <  2:647315/151‑1 (MQ=255)
        aaTCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATgg                                                                                                                                     <  4:137958/151‑1 (MQ=255)
        aaTCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATgg                                                                                                                                     >  2:1350533/1‑151 (MQ=255)
        aaTCAGCGGCTCAACTACGCGGCGCAGCTCTTTCCCTTTAGGCAGAGTCCTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATg                                                                                                                                      <  3:270954/150‑1 (MQ=255)
         aTCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGgc                                                                                                                                    >  2:945090/1‑151 (MQ=255)
          tCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGgc                                                                                                                                    <  1:188150/150‑1 (MQ=255)
             gCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCa                                                                                                                                 <  3:780641/150‑1 (MQ=255)
               ggCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATg                                                                                                                               <  2:686478/150‑1 (MQ=255)
               ggCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGa                                                                                                                              <  3:348026/151‑1 (MQ=255)
                 cTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGAcc                                                                                                                            >  3:833759/1‑151 (MQ=255)
                    aaCTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCCTGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGCTCAGTTGACGACCGCTCTTACGATGGCGCATGACCtt                                                                                                                          >  3:325200/1‑150 (MQ=255)
                        aCGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATcc                                                                                                                      <  2:45760/150‑1 (MQ=255)
                        aCGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCt                                                                                                                     >  2:844431/1‑151 (MQ=255)
                        aCGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCt                                                                                                                     >  2:511593/1‑151 (MQ=255)
                         cgcgGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCtt                                                                                                                    >  3:1289272/1‑151 (MQ=255)
                             gcgcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGACCCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCa                                                                                                                <  3:1089571/151‑1 (MQ=255)
                              cgcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTtctc                                                                                                                 >  4:444375/1‑149 (MQ=255)
                              cgcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAg                                                                                                               <  3:135678/151‑1 (MQ=255)
                               gcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGt                                                                                                              <  2:743408/151‑1 (MQ=255)
                               gcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGt                                                                                                              <  2:514089/151‑1 (MQ=255)
                                  gCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTaa                                                                                                            <  3:1360453/150‑1 (MQ=255)
                                  gCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTaa                                                                                                            >  2:976701/1‑150 (MQ=255)
                                   ctctTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTaaa                                                                                                           <  4:420641/150‑1 (MQ=255)
                                    tctTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAAc                                                                                                         >  2:1013107/1‑151 (MQ=255)
                                      tttCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCt                                                                                                       >  3:537403/1‑151 (MQ=255)
                                        tCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCtt                                                                                                      >  2:75810/1‑150 (MQ=255)
                                         cGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCtt                                                                                                      <  2:178366/149‑1 (MQ=255)
                                          gCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTaa                                                                                                    <  3:1329633/150‑1 (MQ=255)
                                             ttAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAAcc                                                                                                  <  3:294229/149‑1 (MQ=255)
                                             ttAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCtg                                                                                                >  3:12612/1‑151 (MQ=255)
                                             ttAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCt                                                                                                 <  3:193064/150‑1 (MQ=255)
                                              tAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCtg                                                                                                <  4:210245/150‑1 (MQ=255)
                                               aGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCtgtg                                                                                              <  2:119187/151‑1 (MQ=255)
                                                  cAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATc                                                                                           >  2:1369166/1‑151 (MQ=255)
                                                  cAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATc                                                                                           >  3:637075/1‑151 (MQ=255)
                                                   agagTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATc                                                                                           <  2:880588/150‑1 (MQ=255)
                                                     agTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCgg                                                                                        >  3:821166/1‑151 (MQ=255)
                                                      gtcgtcTTGATGATTTCATGACGAACCAGTGAGCCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGt                                                                                       <  2:179743/151‑1 (MQ=255)
                                                      gtcgtcTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCgg                                                                                        <  3:831889/150‑1 (MQ=255)
                                                       tcgtcTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCgg                                                                                        <  2:796028/149‑1 (MQ=255)
                                                           cTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTc                                                                                  >  4:142516/1‑151 (MQ=255)
                                                           cTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTc                                                                                  >  4:226028/1‑151 (MQ=255)
                                                           cTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTc                                                                                  >  1:305884/1‑151 (MQ=255)
                                                             tgatgaTTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTc                                                                                  <  2:1388008/149‑1 (MQ=255)
                                                                 gatTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGc                                                                            >  1:19887/1‑151 (MQ=255)
                                                                  atTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGc                                                                            <  3:1350605/150‑1 (MQ=255)
                                                                   tttCATGGCGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCg                                                                           <  2:460234/150‑1 (MQ=255)
                                                                    ttCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGAt                                                                         >  2:606440/1‑151 (MQ=255)
                                                                     tCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGAt                                                                         <  3:1379660/150‑1 (MQ=255)
                                                                     tCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATg                                                                        >  2:175776/1‑151 (MQ=255)
                                                                        tGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCtt                                                                     >  2:1350004/1‑151 (MQ=255)
                                                                         gACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCtt                                                                     <  3:414889/150‑1 (MQ=255)
                                                                          aCGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCCGTTGCGGTTCAGTTTACGACCTCACTTACGAAGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGTTCCGGTTACTCGTCAGCGATTCTTGc                                                                   >  4:380409/1‑151 (MQ=255)
                                                                           cGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGc                                                                   <  3:290368/150‑1 (MQ=255)
                                                                            gAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCg                                                                 >  3:1188529/1‑151 (MQ=255)
                                                                               ccAGTAAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTg                                                              <  3:902630/151‑1 (MQ=255)
                                                                                cAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTgg                                                             >  2:1291523/1‑151 (MQ=255)
                                                                                  gTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGcc                                                           >  2:908852/1‑151 (MQ=255)
                                                                                    gAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGcc                                                           >  3:1092472/1‑149 (MQ=255)
                                                                                    gAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCa                                                          <  3:51425/150‑1 (MQ=255)
                                                                                     aaCCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGt                                                        >  2:614075/1‑151 (MQ=255)
                                                                                          gCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGtttt                                                     <  3:638360/149‑1 (MQ=255)
                                                                                          gCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGtttt                                                     <  2:413917/149‑1 (MQ=255)
                                                                                            cATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAg                                                 <  1:27678/151‑1 (MQ=255)
                                                                                               atTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGgcg                                              <  2:1259572/151‑1 (MQ=255)
                                                                                               atTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGgc                                               <  3:577229/150‑1 (MQ=255)
                                                                                                ttGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGgcgc                                             >  3:414246/1‑151 (MQ=255)
                                                                                                  gCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCAt                                           >  2:332514/1‑151 (MQ=255)
                                                                                                  gCGGAACATAGCCTCGCGATGGCTGCTGTTGCGGCTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCCGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCAt                                           <  2:325170/151‑1 (MQ=255)
                                                                                                   cGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATg                                          >  2:1071982/1‑151 (MQ=255)
                                                                                                    ggAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGc                                         >  3:735223/1‑151 (MQ=255)
                                                                                                    ggAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGc                                         >  3:312948/1‑151 (MQ=255)
                                                                                                    ggAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGc                                         >  2:429269/1‑151 (MQ=255)
                                                                                                        cATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCag                                     <  1:132972/151‑1 (MQ=255)
                                                                                                          tAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCag                                     >  2:107233/1‑149 (MQ=255)
                                                                                                             ccTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACa                                <  4:321363/151‑1 (MQ=255)
                                                                                                                ggCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAg                               <  3:1291592/149‑1 (MQ=255)
                                                                                                                  cGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTcc                            >  2:516720/1‑150 (MQ=255)
                                                                                                                  cGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCa                           <  2:665073/151‑1 (MQ=255)
                                                                                                                      ggCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACggg                       >  2:169598/1‑151 (MQ=255)
                                                                                                                      ggCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACgg                        <  2:821131/150‑1 (MQ=255)
                                                                                                                         tgctgTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGa                      >  3:394874/1‑149 (MQ=255)
                                                                                                                          gctgTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGaa                     <  3:1316662/149‑1 (MQ=255)
                                                                                                                             gTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGcc                  <  1:48407/149‑1 (MQ=255)
                                                                                                                             gTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCa                 <  3:844468/150‑1 (MQ=255)
                                                                                                                             gTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCa                 <  4:167285/150‑1 (MQ=255)
                                                                                                                              ttGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGc               >  2:135982/1‑151 (MQ=255)
                                                                                                                                 cGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACg            <  3:28307/151‑1 (MQ=255)
                                                                                                                                  ggTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGt           <  2:64593/151‑1 (MQ=255)
                                                                                                                                   gTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTc          >  3:1117952/1‑151 (MQ=255)
                                                                                                                                   gTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTc          >  3:843048/1‑151 (MQ=255)
                                                                                                                                    ttCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGt           >  3:608467/1‑149 (MQ=255)
                                                                                                                                      cAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCtt        <  2:30964/150‑1 (MQ=255)
                                                                                                                                       aGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGTCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTa      <  3:339911/151‑1 (MQ=255)
                                                                                                                                       aGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCttt       >  4:145410/1‑150 (MQ=255)
                                                                                                                                       aGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCttt       >  2:1199623/1‑150 (MQ=255)
                                                                                                                                       aGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCttt       >  2:818535/1‑150 (MQ=255)
                                                                                                                                        gTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTaa     <  4:366480/151‑1 (MQ=255)
                                                                                                                                           gacgacCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAtct  >  2:489995/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCT  >  NC_000913/3439836‑3440125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑