BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,497,615	T→C	F205L (TTC→CTC)	nirC →	nitrite transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,497,615	0	T	C	99.1%	390.4 / NA	107	F205L (TTC→CTC)	nirC	nitrite transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (62/44); minor base A (1/0); total (63/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 108 total aligned reads displayed.

CAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGTTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGCCGAAAGCGAAT  >  NC_000913/3497467‑3497761

cAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTc                                                                                                                                                  <  2:506405/151‑1 (MQ=255)

    ggTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCgcgc                                                                                                                                              >  3:1034859/1‑151 (MQ=255)

     gTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGct                                                                                                                                             >  2:439723/1‑151 (MQ=255)

      tGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGctc                                                                                                                                            >  3:16633/1‑151 (MQ=255)

            gtgtAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTg                                                                                                                                       <  2:279653/150‑1 (MQ=255)

               tAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTc                                                                                                                                   >  2:562190/1‑151 (MQ=255)

               tAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTc                                                                                                                                   >  1:374669/1‑151 (MQ=255)

                aaCTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTc                                                                                                                                   <  3:239799/150‑1 (MQ=255)

                aaCTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTc                                                                                                                                   <  2:1345265/150‑1 (MQ=255)

                aaCTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTc                                                                                                                                   <  3:21020/150‑1 (MQ=255)

                 aCTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCgg                                                                                                                                 >  2:315065/1‑151 (MQ=255)

                    ggctggTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCaa                                                                                                                              <  1:475435/151‑1 (MQ=255)

                    ggctggTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCa                                                                                                                               >  1:286235/1‑150 (MQ=255)

                       tggTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGACATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGCGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAAc                                                                                                                             >  3:420595/1‑149 (MQ=255)

                         gTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACcac                                                                                                                          >  3:1276606/1‑150 (MQ=255)

                         gTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACcac                                                                                                                          >  2:1164537/1‑150 (MQ=255)

                         gTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACcac                                                                                                                          >  2:598645/1‑150 (MQ=255)

                             gCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACGACAGCg                                                                                                                      >  2:967321/1‑150 (MQ=255)

                                  gCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCt                                                                                                                >  3:717575/1‑151 (MQ=255)

                                   cAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTa                                                                                                               <  2:415857/151‑1 (MQ=255)

                                         ggATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACg                                                                                                           <  1:183868/149‑1 (MQ=255)

                                         ggATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACg                                                                                                           <  4:1460/149‑1 (MQ=255)

                                             ggCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGc                                                                                                      <  3:383905/150‑1 (MQ=255)

                                               cgcTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCggg                                                                                                   >  1:434122/1‑151 (MQ=255)

                                               cgcTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCggg                                                                                                   >  4:310123/1‑151 (MQ=255)

                                               cgcTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCCCGCGCTCTCCTGGTTGGGCAACCACAGCGAAGCCTACACGCTGGCg                                                                                                     >  4:60239/1‑149 (MQ=255)

                                                  tGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCgggggt                                                                                                >  4:454189/1‑148 (MQ=255)

                                                   gcgcACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTAt                                                                                                <  2:487327/150‑1 (MQ=255)

                                                   gcgcACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGAtt                                                                                               >  3:611979/1‑151 (MQ=255)

                                                     gcACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTgg                                                                                             >  1:134022/1‑151 (MQ=255)

                                                     gcACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTg                                                                                              >  2:1066006/1‑150 (MQ=255)

                                                        cTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGt                                                                                            >  2:1203364/1‑149 (MQ=255)

                                                         tGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCa                                                                                          >  3:650453/1‑150 (MQ=255)

                                                           aaGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATaa                                                                                       <  1:221351/151‑1 (MQ=255)

                                                                   gcgAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGTTTCGCCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCtgt                                                                               <  2:439212/151‑1 (MQ=255)

                                                                   gcgAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCtgt                                                                               <  2:213001/151‑1 (MQ=255)

                                                                   gcgAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACctgctg                                                                                >  1:265199/1‑150 (MQ=255)

                                                                     gAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTgg                                                                             <  1:82533/151‑1 (MQ=255)

                                                                     gAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTgg                                                                             <  2:224072/151‑1 (MQ=255)

                                                                      aaaTTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAAACAGCTGTgg                                                                             >  2:124950/1‑150 (MQ=255)

                                                                         tttATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGTggg                                                                            >  2:290964/1‑148 (MQ=255)

                                                                          ttATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGGGAc                                                                        >  2:354857/1‑151 (MQ=255)

                                                                           tATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGGGGGTGACg                                                                       >  3:550997/1‑151 (MQ=255)

                                                                             tCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGGGAc                                                                        >  3:80245/1‑148 (MQ=255)

                                                                              cGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGGGACGCt                                                                     >  3:411053/1‑150 (MQ=255)

                                                                               gCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTgg                                                                   <  3:315095/151‑1 (MQ=255)

                                                                                cTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGTGGGGGACGCTggg                                                                  >  3:133273/1‑151 (MQ=255)

                                                                                  aTCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTggg                                                                  <  3:746789/149‑1 (MQ=255)

                                                                                  aTCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGt                                                                 <  3:109189/150‑1 (MQ=255)

                                                                                      ggtggtGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTa                                                                <  2:147682/147‑1 (MQ=255)

                                                                                      ggtggtGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAAt                                                              <  2:743585/149‑1 (MQ=255)

                                                                                       gtggtgTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTTGGCATAAACTGCTGTGGGTGACGCTGGGTAATAc                                                            >  1:211157/1‑150 (MQ=255)

                                                                                        tggtgTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAAACTGCTGTGGGGGACGCTGGGTAAAACtt                                                          >  3:979789/1‑151 (MQ=255)

                                                                                         ggtgTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACttt                                                         <  3:404295/151‑1 (MQ=255)

                                                                                         ggtgTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACtt                                                          <  3:196890/150‑1 (MQ=255)

                                                                                          gtgtCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTa                                                        <  3:439757/151‑1 (MQ=255)

                                                                                           tgtCTGCTGGCATTTATCGCGTCCGGCTATGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGGGACGCTGGGGAATACTTTAt                                                       >  2:955311/1‑151 (MQ=255)

                                                                                           tgtCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGGAATACTTTAt                                                       >  3:107846/1‑151 (MQ=255)

                                                                                            gtCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATc                                                      <  2:1402243/151‑1 (MQ=255)

                                                                                            gtCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTAGCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTAt                                                       <  2:1096832/150‑1 (MQ=255)

                                                                                             tCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCCGCTGTGGGGGACGCTGGGGAATACTTTAt                                                       >  2:731768/1‑149 (MQ=255)

                                                                                                  tgGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTTGTTATAACCTGCTGTGGGTGACGCCGGGGAATACTTTATTAGGt                                                  >  3:793088/1‑149 (MQ=255)

                                                                                                    gCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCg                                              <  2:420565/151‑1 (MQ=255)

                                                                                                     cATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGGGGGGGGCGGTGGGGAAAACTTTATTAGGGGCCg                                              >  2:442349/1‑150 (MQ=255)

                                                                                                      aTTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTa                                            <  3:1252153/151‑1 (MQ=255)

                                                                                                      aTTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAAACTGCTGTGGGTGACGCTGGGGAATACTTTTTCAGGTGCCg                                              >  2:67818/1‑149 (MQ=255)

                                                                                                          aTCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCa                                        <  2:211721/151‑1 (MQ=255)

                                                                                                          aTCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCa                                        <  1:353293/151‑1 (MQ=255)

                                                                                                            cgcgTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGGGACGCTGGGTAATAATTTATCAGGTGCCGGATTTATg                                      >  1:408850/1‑151 (MQ=255)

                                                                                                            cgcgTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGTGGGGGACGCTGGGGAATACTTTTTCAGGTGCCGTATTCATg                                      >  3:130585/1‑151 (MQ=255)

                                                                                                               gTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGGGGCGCTGGGGAATACTTTATCAGGGGCCGTATTCCTggg                                    >  3:407959/1‑150 (MQ=255)

                                                                                                               gTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGGGACGCTGGGGAATACTTTATCAGGTGCCGTATTCCTggg                                    >  3:24981/1‑150 (MQ=255)

                                                                                                                   ggCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTgg                               <  3:280172/151‑1 (MQ=255)

                                                                                                                   ggCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGGGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGAtt                                 >  1:1752/1‑149 (MQ=255)

                                                                                                                    gCTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTggg                              <  2:483817/151‑1 (MQ=255)

                                                                                                                     cTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAAACTGCTGTGGGTGACGCTGGGGAATACTTTATCAGGGGCCGGATTCATGGGGTTggg                              >  3:1172024/1‑150 (MQ=255)

                                                                                                                     cTACGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAAACTGCTGTGGGGGGCGCTGGGTAATACTTTATCAGGGGCCGTATTCATGGGATTggg                              >  2:855031/1‑150 (MQ=255)

                                                                                                                       aCGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCCTAACCTGCTGTGGGGGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGtt                            >  2:711087/1‑150 (MQ=255)

                                                                                                                       aCGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGt                             <  3:1394006/149‑1 (MQ=255)

                                                                                                                       aCGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCCGCTGTGGGTGACGCTGGGGAATACTTTTTCAGGGGCCGTATTCATGGGGTTggg                              >  3:1088294/1‑148 (MQ=255)

                                                                                                                       aCGAGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTTGTCCTAAACTGCTGTGGGTGACGCCTGGGAATGCTTTATCAGGTGCCGTATTCATGGGGTTggggt                            >  3:507844/1‑148 (MQ=255)

                                                                                                                          aGCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATTAACTGCTGTGGGGGACGCTGGGGAATACTTTATCAGGTGGCGTATTTATGGGGTTGGGGTAtt                         >  3:1264403/1‑150 (MQ=255)

                                                                                                                           gCACTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTTTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGGTTGGGGTAt                          >  3:1005807/1‑148 (MQ=255)

                                                                                                                             aCTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTa                     <  2:611952/151‑1 (MQ=255)

                                                                                                                             aCTCTATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTa                     <  3:764665/151‑1 (MQ=255)

                                                                                                                                ctATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTAt                    <  3:467561/149‑1 (MQ=255)

                                                                                                                                 tATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTTGTCCTAAACTGCTGGGGGTGACGCTGGGTAATACTTTATCAGGTGCCCTATTCATGGGATTGGGGTATTGGTa                     >  2:1287697/1‑147 (MQ=255)

                                                                                                                                 tATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGGTTGGGGTATTGGTATGc                  >  2:458329/1‑150 (MQ=255)

                                                                                                                                 tATCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTTGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGGATTCATGGGGTTGGGGTATTGGTATGc                  >  3:122753/1‑150 (MQ=255)

                                                                                                                                  aTCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGGCATAACCTGCTGTGGGGGACGCTGGGGAATACTTTATCAGGTGCCGTATTCCTGGGGTTGGGGTATTGGTATGCt                 >  2:504377/1‑150 (MQ=255)

                                                                                                                                   tCGCTAACATGACGCTGCTCGCTCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATg                   <  3:610814/147‑1 (MQ=255)

                                                                                                                                   tCGCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTTGTCCTTACCTGCTGTGGGGGACGCTGGGGAATACTTTATCAGGTGCCGTATTCATGGGGTTGGGGTTTTGGTATTCTAc               >  3:688423/1‑151 (MQ=255)

                                                                                                                                     gCTAACATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAAACTGCTGTGGGGGACGCTGGGCAATACTTTATCAGGTGCCGTATTCATGGGGTTGGGGTATTGGGATGCTAc               >  1:91386/1‑149 (MQ=255)

                                                                                                                                        aaCATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGcc            <  4:25420/149‑1 (MQ=255)

                                                                                                                                        aaCATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTTGTTATAAACCACTGTGGGGGACGCCGGGTAAAACTTTATCAGGTGCCGTATTCATGGGATTTGGGTATTTGTAAGCCACGcc            >  4:187029/1‑149 (MQ=255)

                                                                                                                                         aCATGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGCCGaa         <  2:996715/151‑1 (MQ=255)

                                                                                                                                           aTGACGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGCCGAAAg       <  1:338444/151‑1 (MQ=255)

                                                                                                                                              aCGCTGATCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGGATTGGTCATAACCTGCTGTGGGGGACACTGGGGAATAATTTATAAGGTGGCGTATTCATGGGGTTGGGTTATTGGTATGCTACGCCGAAAGCg     >  2:100218/1‑150 (MQ=255)

                                                                                                                                               cGCTGCTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGGAATACTTTATCAGGGGCCGTATTCATGGGGTTGGGTTATTGGTATGCTACGCCGaa         >  3:165967/1‑145 (MQ=255)

                                                                                                                                                gctgctCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGCCGAAAGCGAAt  <  3:120137/151‑1 (MQ=255)

CAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGTTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGTTATTGGTATGCTACGCCGAAAGCGAAT  >  NC_000913/3497467‑3497761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`