Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,773,127 A→G T283A (ACG→GCG)  mtlA → mannitol‑specific PTS enzyme: IIA, IIB and IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,773,1270AG100.0% 373.8 / NA 104T283A (ACG→GCG) mtlAmannitol‑specific PTS enzyme: IIA, IIB and IIC components
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (53/51);  total (53/51)

Only 100 of 104 total aligned reads displayed.

GGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCT  >  NC_000913/3772983‑3773273
                                                                                                                                                |                                                                                                                                                  
   cGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACt                                                                                                                                            <  2:756323/150‑1 (MQ=255)
   cGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTa                                                                                                                                           <  4:235099/151‑1 (MQ=255)
    gTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTAt                                                                                                                                          <  2:153689/151‑1 (MQ=255)
         aGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTg                                                                                                                                      <  2:797928/150‑1 (MQ=255)
          gcgcTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTggg                                                                                                                                    <  4:309420/151‑1 (MQ=255)
            gcTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCg                                                                                                                                  <  2:1246806/151‑1 (MQ=255)
             cTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCgg                                                                                                                                 >  2:1211564/1‑151 (MQ=255)
              tAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCgg                                                                                                                                 >  3:937651/1‑150 (MQ=255)
              tAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCgg                                                                                                                                 >  3:237718/1‑150 (MQ=255)
               aaaCAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCggt                                                                                                                                <  3:260625/150‑1 (MQ=255)
                 aCAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCggtgg                                                                                                                              >  2:62109/1‑150 (MQ=255)
                   aGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCt                                                                                                                           <  2:517811/151‑1 (MQ=255)
                   aGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCt                                                                                                                           <  2:299974/151‑1 (MQ=255)
                     tCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTgg                                                                                                                         >  4:117187/1‑151 (MQ=255)
                      cTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGt                                                                                                                        >  2:1172944/1‑151 (MQ=255)
                        gcgggcggTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGttt                                                                                                                      >  4:446439/1‑151 (MQ=255)
                        gcgggcggTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGt                                                                                                                        <  2:1021642/149‑1 (MQ=255)
                          gggcggTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTtc                                                                                                                     >  3:329706/1‑150 (MQ=255)
                           ggcggTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTtctc                                                                                                                   <  3:982127/151‑1 (MQ=255)
                             cggTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTcc                                                                                                                  >  3:42730/1‑150 (MQ=255)
                                 gCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCAt                                                                                                             >  3:1199487/1‑151 (MQ=255)
                                 gCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCAt                                                                                                             >  2:139342/1‑151 (MQ=255)
                                 gCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCAt                                                                                                             <  3:956440/151‑1 (MQ=255)
                                  cGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCAtc                                                                                                            >  2:833987/1‑151 (MQ=255)
                                   ggCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGCTTCTCCGGCAtc                                                                                                            >  2:1066600/1‑150 (MQ=255)
                                     cAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTcc                                                                                                         <  3:331022/151‑1 (MQ=255)
                                         catcCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGt                                                                                                     >  3:728867/1‑151 (MQ=255)
                                            ccACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCt                                                                                                  <  4:433967/151‑1 (MQ=255)
                                             cACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCt                                                                                                  >  4:419648/1‑150 (MQ=255)
                                             cACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCt                                                                                                  >  3:78474/1‑150 (MQ=255)
                                               cTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTAt                                                                                                <  2:642564/150‑1 (MQ=255)
                                               cTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATc                                                                                               >  3:569187/1‑151 (MQ=255)
                                                 tCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATcc                                                                                              <  1:145794/150‑1 (MQ=255)
                                                 tCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGGTTCTCCGGCATCTCCGGGTTCTATcc                                                                                              >  3:621296/1‑150 (MQ=255)
                                                   cTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCtt                                                                                            >  3:1352012/1‑150 (MQ=255)
                                                   cTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTg                                                                                           >  2:462396/1‑151 (MQ=255)
                                                    tGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGc                                                                                          >  4:438713/1‑151 (MQ=255)
                                                     ggggggTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGc                                                                                          <  1:437828/150‑1 (MQ=255)
                                                     ggggggTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCt                                                                                         <  2:355480/151‑1 (MQ=255)
                                                      gggggTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTg                                                                                        <  4:478307/151‑1 (MQ=255)
                                                       ggggTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGt                                                                                       <  3:633755/151‑1 (MQ=255)
                                                       ggggTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGGTCTATCCTTGCTGt                                                                                       >  3:1212371/1‑151 (MQ=255)
                                                        gggTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTa                                                                                      <  3:1211625/151‑1 (MQ=255)
                                                            aTCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTgg                                                                                  <  2:101322/151‑1 (MQ=255)
                                                             tCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGc                                                                                 >  4:460514/1‑151 (MQ=255)
                                                              ccACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCg                                                                                <  2:684444/151‑1 (MQ=255)
                                                                 cGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATg                                                                             <  4:144328/151‑1 (MQ=255)
                                                                    aaTCTCCTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGTTTCTATCCTTGCTGTACTGGCGATGaca                                                                          <  3:552593/151‑1 (MQ=255)
                                                                      tCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACAcc                                                                        >  3:1111894/1‑151 (MQ=255)
                                                                        tACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCa                                                                       >  2:714741/1‑150 (MQ=255)
                                                                         aCTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGGGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCaa                                                                      >  1:425519/1‑150 (MQ=255)
                                                                            tCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAg                                                                   <  3:126638/150‑1 (MQ=255)
                                                                             cccGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAgg                                                                  >  2:213043/1‑150 (MQ=255)
                                                                             cccGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGt                                                                 <  3:944399/151‑1 (MQ=255)
                                                                             cccGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGCCTGGTTTCCCCGGCATCTCCGGGTTCTCTCCTTGCTGTACTGGCGATGACACCAAAAGGt                                                                 >  3:1035749/1‑151 (MQ=255)
                                                                              ccGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTg                                                                <  1:92094/151‑1 (MQ=255)
                                                                              ccGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTg                                                                >  3:180987/1‑151 (MQ=255)
                                                                                  aTGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCtt                                                             >  4:415004/1‑150 (MQ=255)
                                                                                  aTGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCtt                                                             <  2:531849/150‑1 (MQ=255)
                                                                                  aTGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCtt                                                             <  1:396664/150‑1 (MQ=255)
                                                                                   tgtgCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTAc                                                           >  3:945090/1‑151 (MQ=255)
                                                                                    gtgCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTAc                                                           <  2:479526/150‑1 (MQ=255)
                                                                                      gCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACtt                                                         <  1:353532/150‑1 (MQ=255)
                                                                                       cTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCg                                                       >  4:107055/1‑151 (MQ=255)
                                                                                           tgaATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTa                                                    >  2:448518/1‑150 (MQ=255)
                                                                                            gaATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAAc                                                  <  3:434955/151‑1 (MQ=255)
                                                                                              aTCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACAt                                                <  2:852122/151‑1 (MQ=255)
                                                                                                      cTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGgtg                                        >  1:114704/1‑151 (MQ=255)
                                                                                                       tGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATc                                               <  3:614455/143‑1 (MQ=255)
                                                                                                       tGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATc                                               >  2:614428/1‑143 (MQ=255)
                                                                                                       tGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGgtg                                        >  3:1088876/1‑150 (MQ=255)
                                                                                                        gATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGgt                                         <  2:793558/148‑1 (MQ=255)
                                                                                                         aTCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGgtgtg                                      >  1:404694/1‑150 (MQ=255)
                                                                                                           ccTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGgtgtgtgt                                   <  1:63936/151‑1 (MQ=255)
                                                                                                             tCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTgc                                 >  4:141317/1‑151 (MQ=255)
                                                                                                              cGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTgcg                                <  2:1141392/151‑1 (MQ=255)
                                                                                                              cGCAGTCATCCTCGGCGGTATGACTGGCGGGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGGTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGGGTGTGTgcg                                >  4:211087/1‑151 (MQ=255)
                                                                                                               gCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTgcgg                               <  2:570983/151‑1 (MQ=255)
                                                                                                                cAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTgcggc                              >  4:134689/1‑151 (MQ=255)
                                                                                                                 aGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCCAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTgcggc                              >  3:920841/1‑150 (MQ=255)
                                                                                                                 aGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTgcggcg                             <  3:1307553/151‑1 (MQ=255)
                                                                                                                 aGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTgcgg                               <  2:167620/149‑1 (MQ=255)
                                                                                                                  gTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGCTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTgcggc                              >  3:304406/1‑149 (MQ=255)
                                                                                                                     aTCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATgg                         >  3:509956/1‑151 (MQ=255)
                                                                                                                     aTCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATgg                         <  3:1157968/151‑1 (MQ=255)
                                                                                                                      tCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATgg                         >  2:466807/1‑150 (MQ=255)
                                                                                                                          cggcggTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGtc                    <  2:787576/151‑1 (MQ=255)
                                                                                                                           ggcggTATGACTGGCGTGTTCGCGCTGGCTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGtc                    >  4:228187/1‑150 (MQ=255)
                                                                                                                              ggTATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCt                >  2:422786/1‑151 (MQ=255)
                                                                                                                                tATGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTc              >  1:443782/1‑151 (MQ=255)
                                                                                                                                 aTGACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTc              <  2:937613/150‑1 (MQ=255)
                                                                                                                                   gACTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGtt           <  4:214330/151‑1 (MQ=255)
                                                                                                                                     cTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTg          >  4:158774/1‑150 (MQ=255)
                                                                                                                                     cTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTg          <  3:593288/150‑1 (MQ=255)
                                                                                                                                     cTGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGt         >  2:487747/1‑151 (MQ=255)
                                                                                                                                      tGGCGTGTTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGtc        >  2:762282/1‑151 (MQ=255)
                                                                                                                                          gtgtTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTctct     <  2:1035701/150‑1 (MQ=255)
                                                                                                                                           tgtTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGc   >  4:197091/1‑151 (MQ=255)
                                                                                                                                            gtTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCt  >  2:1012149/1‑151 (MQ=255)
                                                                                                                                            gtTCGCGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCt  >  1:148584/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCT  >  NC_000913/3772983‑3773273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑