Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,721	(G)7→6	intergenic (‑265/+44)	ydaG ← / ← racR	Rac prophage; uncharacterized protein/Rac prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,715	0	G	.	100.0%	150.5 / NA	32	intergenic (‑259/+50)	ydaG/racR	Rac prophage; uncharacterized protein/Rac prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (18/14);  total (18/14)

AAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCGCTGCTATGTAGGCG  >  NC_000913/1419580‑1419861                                                                                                                                        |                                                                                                                                                   aaGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACg                                                                                                                                    <  1:495915/151‑1 (MQ=255)    tATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGGGAT‑GGGGGGTTAAATAACGGa                                                                                                                                  >  1:82275/1‑150 (MQ=255)     aTTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAAt                                                                                                                                <  1:271623/151‑1 (MQ=255)                 tCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGtttt                                                                                                                     <  1:1117/150‑1 (MQ=255)                  cTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTcc                                                                                                                   >  2:662633/1‑151 (MQ=255)                    gtgGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGt                                                                                                                 >  1:539753/1‑151 (MQ=255)                       gggCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAg                                                                                                              <  1:144423/151‑1 (MQ=255)                           ggggTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGAcc                                                                                                           >  2:271000/1‑150 (MQ=255)                                    ggtATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagtt                                                                                                  <  1:382381/150‑1 (MQ=255)                                        tGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATAtaagtttaagt                                                                                             >  1:587885/1‑151 (MQ=255)                                             gAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAg                                                                                        <  2:452614/151‑1 (MQ=255)                                                         aTGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGt                                                                            >  2:157555/1‑151 (MQ=255)                                                               aaTTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTgg                                                                       <  2:197074/150‑1 (MQ=255)                                                                 ttgattgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGt                                                                                                                 <  2:661618/106‑1 (MQ=255)                                                                 ttgattgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGt                                                                                                                 >  1:661639/1‑106 (MQ=255)                                                                  tgattgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCttt                                                                   >  1:511077/1‑151 (MQ=255)                                                                   gattgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCtttt                                                                  <  1:476849/151‑1 (MQ=255)                                                                    attgTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTc                                                                 <  1:646508/151‑1 (MQ=255)                                                                          aaTAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTc                                                            >  1:546191/1‑150 (MQ=255)                                                                          aaTAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTc                                                            >  1:139325/1‑150 (MQ=255)                                                                             aGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGAt                                                        <  1:315006/151‑1 (MQ=255)                                                                                  aTGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCaa                                                    <  1:124498/150‑1 (MQ=255)                                                                                      cTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCt                                               >  1:463044/1‑151 (MQ=255)                                                                                        aaTGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCttt                                             >  1:266114/1‑151 (MQ=255)                                                                                           gTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTc                                            >  2:226941/1‑149 (MQ=255)                                                                                           gTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTc                                            >  1:257077/1‑149 (MQ=255)                                                                                                      aaCCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTAccc                                <  1:162660/150‑1 (MQ=255)                                                                                                      aaCCTAATAGGTGATGGCGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTAccc                                >  1:226239/1‑150 (MQ=255)                                                                                                                       cGACAGCAGAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCgct              >  1:654244/1‑151 (MQ=255)                                                                                                                              agAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCGCTGCTATGt       >  1:654550/1‑151 (MQ=255)                                                                                                                              agAAAGTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCGCTGCTATGt       >  1:27815/1‑151 (MQ=255)                                                                                                                                   gTGAT‑GGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCGCTGCTATGTAGgcg  <  2:120138/151‑1 (MQ=255)                                                                                                                                        |                                                                                                                                                   AAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTGTCAATAGGCTATGCCTAATGTTTTGAGCGTAACCTAATAGGTGATGGCGACAGCAGAAAGTGATGGGGGGGTTAAATAACGGAATCCAGGAGTTTTCCGTCAGACCATATAAGTTTAAGTTCCAGTTTTTGTGATGTTCTGGCTTTTCCGTTCAGATTCAAGAGCTTTCAGATACTTACCCACTTTCATTTCCATCGCTGCTATGTAGGCG  >  NC_000913/1419580‑1419861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑