| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,682,157 | (C)7→8 | coding (589/3282 nt) | yphG ← | DUF4380 domain‑containing TPR repeat protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,682,150 | 1 | . | C | 97.1% | 120.4 / ‑2.5 | 35 | coding (596/3282 nt) | yphG | DUF4380 domain‑containing TPR repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (20/14); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01 | |||||||||||
CAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCC‑GGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGC > NC_000913/2682001‑2682293 | cAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCC‑GGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCtt > 1:673147/1‑150 (MQ=255) tttttATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCC‑GGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑cccccccct > 1:40286/1‑149 (MQ=255) ttttAAAGGGAAAAATGCCCACCCCAGGGGAGAAGCCCCTTTTGTAGTAGGTGCG‑GGTGGGGATGGGGAAAG‑GGGGGCGGGCCCTTTGGCGGGGCCAAACCCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑ccccccc < 2:501541/148‑1 (MQ=255) ttATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCC‑GGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACACTCTGATGCCCTT‑‑CCCCCCCctttc > 1:237320/1‑149 (MQ=255) gCGAGAAATGTCCACCCCAGCGGAGTAGTCCATTTTGTAGTAAGTGCG‑GGTAGCGATGGGGAAAGGGGAGACGGCCTTTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGc < 1:53052/151‑1 (MQ=255) tGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCC‑GGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTgg > 1:132930/1‑151 (MQ=255) tGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCC‑GGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTgg > 1:216803/1‑151 (MQ=255) ccACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCC‑GGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGccc > 2:209635/1‑151 (MQ=255) ccAGCGGAGTAGTCCACTTTGTAGTAAGTGCC‑GGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCcaccac > 2:411746/1‑151 (MQ=255) gCGGAGTAGTCCATTTTTTAGTATGTGCG‑GGAGGGGGGGGGAAAAGGGGAGACGCCCTTTTTGCGGTGATAAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGtt < 2:7165/138‑1 (MQ=255) agtCCACTTTGTAGTAAGTGCC‑GGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGAc > 2:448299/1‑151 (MQ=255) cACTTTGTAGTAA‑TGCCGGGTAGCGAGGGGAAAAGCGGAAACGGCCCGTTTGCCGTGATCAAA‑CTCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGAcg < 1:404758/148‑1 (MQ=255) taAGTGCC‑GGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTc > 1:490734/1‑151 (MQ=255) ggaggggaggggaAAAGGGGGGGCGCCCTGTTTGCGTTGACAAAC‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTtat < 2:178858/141‑1 (MQ=255) tAGCGATGGGGAAAGGGGAGACGGCCCGTTTGCCGTGATAAAC‑CCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAg < 1:183885/150‑1 (MQ=255) cGAGGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGAcg > 2:663405/1‑151 (MQ=255) gggAAGGCGGAGACGGCCCTTTTGCGGTGATAAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTg < 1:50339/151‑1 (MQ=255) gggAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTg > 1:304744/1‑151 (MQ=255) gAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTgg > 2:452305/1‑150 (MQ=255) gcccttttggctgTGATAAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAgc < 1:642799/137‑1 (MQ=255) ccGTAATCAAC‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTcc < 1:11/151‑2 (MQ=255) gTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAgg > 1:642684/1‑151 (MQ=255) gTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAgg > 1:152795/1‑151 (MQ=255) ggctacaacggccgccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCa < 1:123603/139‑1 (MQ=255) aacAAA‑CCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGt < 2:296757/142‑1 (MQ=255) tCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg > 2:97863/1‑150 (MQ=255) aa‑CCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgc < 2:493818/146‑1 (MQ=255) cacCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAggg > 2:620659/1‑150 (MQ=255) ttACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTg > 1:55597/1‑151 (MQ=255) ggcggGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCAcc > 1:43541/1‑151 (MQ=255) ggcggGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCAcc < 1:297154/151‑1 (MQ=255) aaGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTaa > 2:157805/1‑150 (MQ=255) gCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATg > 2:125779/1‑151 (MQ=255) tctGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGc > 2:243190/1‑150 (MQ=255) gATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCa < 1:197052/148‑1 (MQ=255) aTGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCgg > 1:92948/1‑151 (MQ=255) aTGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCgg > 1:455906/1‑151 (MQ=255) gCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGc < 1:349142/150‑1 (MQ=255) | CAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCC‑GGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAA‑CACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGC > NC_000913/2682001‑2682293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |