| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,532,256 | +T | coding (184/1725 nt) | yhgE ← | DUF4153 family putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,532,253 | 1 | . | T | 84.4% | 145.2 / 21.7 | 45 | coding (187/1725 nt) | yhgE | DUF4153 family putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (4/3); new base T (23/15); total (27/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
CATTGCCATCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGACCCTGAAGTAATCCGGT > NC_000913/3532104‑3532391 | caTTGCCATCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑t > 2:400945/1‑151 (MQ=255) tGCCATCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑ttt < 1:408134/150‑1 (MQ=255) ccATCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGGCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaa < 1:524951/151‑1 (MQ=255) cATCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaaa < 2:126132/151‑1 (MQ=255) aTCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaaa > 1:94133/1‑150 (MQ=255) aaCAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCTCTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAagag < 2:165307/151‑1 (MQ=255) aaCAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAagag < 2:68985/151‑1 (MQ=255) aaCAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaaaag > 1:673665/1‑147 (MQ=255) aCAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAgaga < 2:452455/151‑1 (MQ=255) agcagATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaaaag > 1:664043/1‑144 (MQ=255) tAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACgg > 2:73653/1‑149 (MQ=255) aaCCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGgaga > 1:197317/1‑148 (MQ=255) ccAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGt > 1:151492/1‑151 (MQ=255) aaaaTCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTaa < 1:218103/151‑1 (MQ=255) aaaTCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACAGAGAAGAGTaa > 2:520810/1‑150 (MQ=255) aaTCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTaaaa < 2:563737/151‑1 (MQ=255) gcCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAAc > 2:286135/1‑151 (MQ=255) cAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACgg > 1:286288/1‑151 (MQ=255) cAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACg < 2:178298/150‑1 (MQ=255) aGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaaa < 2:510431/109‑1 (MQ=255) aGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTtaaa > 1:510445/1‑109 (MQ=255) ctcCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGc > 2:191799/1‑150 (MQ=255) aGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAgg > 2:619095/1‑149 (MQ=255) aGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGc > 1:522625/1‑150 (MQ=255) gggTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACg < 1:79897/151‑1 (MQ=255) aTGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATa > 2:645367/1‑150 (MQ=255) cATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACa > 1:248585/1‑150 (MQ=255) aTCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAAt > 2:512091/1‑151 (MQ=255) ttGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTAGCAGGCACGCCATAAACAATCCAACt > 2:244380/1‑151 (MQ=255) aCTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGAt > 1:603236/1‑151 (MQ=255) agccagccGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGAtttttt < 1:653739/151‑1 (MQ=255) ccagccGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTcc < 1:220323/151‑1 (MQ=255) cGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGaa > 2:201663/1‑150 (MQ=255) tCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGcc > 1:518748/1‑151 (MQ=255) aGCGGGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGAt < 2:393408/150‑1 (MQ=255) cAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAgg > 2:498455/1‑150 (MQ=255) cAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGt < 1:269441/151‑1 (MQ=255) aaaCACTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCa < 2:664022/151‑1 (MQ=255) cTAGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAAcc > 2:620416/1‑150 (MQ=255) aGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAAcc > 1:173970/1‑148 (MQ=255) aGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCa < 1:45884/149‑1 (MQ=255) aGCGCCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAg < 2:601722/150‑1 (MQ=255) cgcCAGCCATCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGAcc > 2:87001/1‑151 (MQ=255) cagccaTCCCCAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGACCCTg > 1:214590/1‑151 (MQ=255) ccccAAAGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGACCCTGAAGTaa < 1:216102/150‑1 (MQ=255) aaaGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGACCCTGAAGTAATCCgg > 2:421059/1‑151 (MQ=255) aGGCGCTTCTGTTTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGACCCTGAAGTAATCCGGt > 1:75681/1‑150 (MQ=255) | CATTGCCATCAACAGCAGATAGCAACCAAAATCCCAAAGCGCCTTTTCAGCTCTCCAGGGGTTCATGCCATCAGTTTGCCACTTCAGCCAGCCGCTCATCCCCAGCGTGGCAATAAACACTAGCGCCAGCCATCCCCAAAGGCGCTTCTG‑TTTAAAAGAGATCACGGAGAAGAGTAAAACGGATGAAAACGCGACGGTCGCAGGCACGCCATAAACAATCCAACTGTGATTTTTTCCGGAAAGCCAGGCGATCAGCAGGTAGCAAACCAGACCCTGAAGTAATCCGGT > NC_000913/3532104‑3532391 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |