Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	484,337	(C)6→7	coding (67/3150 nt)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	484,331	1	.	C	100.0%	112.2 / NA	32	L25V (CTG→GTG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (17/15);  total (17/15)

GTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAG‑CCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGT  >  NC_000913/484182‑484472                                                                                                                                                       |                                                                                                                                              gTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAG‑c                                                                                                                                              <  2:225252/151‑1 (MQ=255)   tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATGTTGCGCCACCGGCAGTTTGAGGATCGCCAG‑ccc                                                                                                                                            >  1:635085/1‑151 (MQ=255)   tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAG‑cc                                                                                                                                             <  2:437111/150‑1 (MQ=255)         tACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAG‑ccccccct                                                                                                                                       >  2:79837/1‑148 (MQ=255)                          tCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAAt                                                                                                                       <  1:301940/150‑1 (MQ=255)                               aaCCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGAt                                                                                                                 <  2:114395/151‑1 (MQ=255)                                aCCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATc                                                                                                                <  2:408163/151‑1 (MQ=255)                                     tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCAccc                                                                                                            >  2:431568/1‑150 (MQ=255)                                      gtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCAc                                                                                                          <  1:316817/151‑1 (MQ=255)                                          ccGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCa                                                                                                       >  2:42673/1‑150 (MQ=255)                                           cGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCCACATGATGATAATGGCGATCACCCACGCaa                                                                                                      >  2:93309/1‑150 (MQ=255)                                                     aCTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcg                                                                                            >  1:462198/1‑150 (MQ=255)                                                      cTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgat                                                                                          >  2:183807/1‑151 (MQ=255)                                                       tGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgatc                                                                                         >  2:504749/1‑151 (MQ=255)                                                        gTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGCATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgatcg                                                                                        <  2:628880/151‑1 (MQ=255)                                                           ttCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgatcgat                                                                                      <  1:533212/150‑1 (MQ=255)                                                            tCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATa                                                                                     <  2:507968/150‑1 (MQ=255)                                                              gCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAg                                                                                  <  2:643648/151‑1 (MQ=255)                                                                aTCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGaa                                                                                <  1:183812/151‑1 (MQ=255)                                                                   aGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAAtt                                                                             <  1:379575/151‑1 (MQ=255)                                                                     cgcCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAAAATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAAtt                                                                             >  1:380978/1‑149 (MQ=255)                                                                      gcCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAgg                                                                          <  1:662813/151‑1 (MQ=255)                                                                       ccGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGc                                                                         >  1:428665/1‑151 (MQ=255)                                                                              aggaggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCCGGCGATCGATAAAGAAATTAGGCATGTCtt                                                                  >  2:466925/1‑151 (MQ=255)                                                                                        gaTCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTcc                                                         >  2:584304/1‑150 (MQ=255)                                                                                              tACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTtta                                                   >  2:455368/1‑150 (MQ=255)                                                                                                 tGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATTATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTtttagtt                                               >  2:671168/1‑151 (MQ=255)                                                                                                         gTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTg                                       <  2:15447/151‑1 (MQ=255)                                                                                                                            cgcCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCAc                    >  1:581820/1‑151 (MQ=255)                                                                                                                                  cGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTg              >  2:399304/1‑151 (MQ=255)                                                                                                                                     cAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCgg           <  1:85019/151‑1 (MQ=255)                                                                                                                                       gTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCt         >  2:164852/1‑151 (MQ=255)                                                                                                                                         ttGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTg       >  1:489764/1‑151 (MQ=255)                                                                                                                                            aGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTg    >  2:284397/1‑151 (MQ=255)                                                                                                                                             ggATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTgg   <  2:618527/151‑1 (MQ=255)                                                                                                                                               aTCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGt  <  1:671560/150‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                              GTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAG‑CCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGT  >  NC_000913/484182‑484472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑