Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,494,883	(A)7→8	coding (261/564 nt)	idnK →	D‑gluconate kinase, thermosensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,494,876	1	.	A	100.0%	144.9 / NA	37	L85* (TTA→TAA)	idnK	D‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (18/19);  total (18/19)

ACGATCTTCATCCAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑AAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGTTACTAAAAAGTCAGT  >  NC_000913/4494726‑4495019                                                                                                                                                        |                                                                                                                                                aCGATCTTCATCCAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAtt                                                                                                                                                  <  1:448341/151‑1 (MQ=255) aCGATCTTCATCCAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTATATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAtt                                                                                                                                                  <  2:138523/151‑1 (MQ=255)     tCTTCATCCAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑aaa                                                                                                                                              >  1:436287/1‑150 (MQ=255)        tCATCCAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATCCAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑aaaaaa                                                                                                                                           <  2:311142/150‑1 (MQ=255)        tCATCCAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑aaaa                                                                                                                                             <  2:476173/148‑1 (MQ=255)         cATCCAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑aaaaaaa                                                                                                                                          <  1:281926/150‑1 (MQ=255)            ccAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑aaaaaaaaca                                                                                                                                       <  1:99624/150‑4 (MQ=255)                ccAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATc                                                                                                                                   <  1:433379/151‑1 (MQ=255)                 cAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCg                                                                                                                                  >  2:473765/1‑151 (MQ=255)                 cAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCg                                                                                                                                  <  1:353302/151‑1 (MQ=255)                   aaaaTATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTg                                                                                                                                <  1:488122/151‑1 (MQ=255)                    aaaTATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTg                                                                                                                                >  2:134696/1‑150 (MQ=255)                              aaaaTGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACg                                                                                                                      <  1:426565/150‑1 (MQ=255)                                             aTTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCAt                                                                                                      <  2:342581/151‑1 (MQ=255)                                                 cATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTc                                                                                                  >  2:74918/1‑151 (MQ=255)                                                 cATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTc                                                                                                  >  1:67013/1‑151 (MQ=255)                                                  aTTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCa                                                                                                 <  2:470124/151‑1 (MQ=255)                                                  aTTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCa                                                                                                 <  1:382560/151‑1 (MQ=255)                                                    tATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAt                                                                                                <  1:341646/150‑1 (MQ=255)                                                                aTCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                                                                     >  2:529857/1‑149 (MQ=255)                                                                  cGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGa                                                                                  <  2:275656/150‑1 (MQ=255)                                                                         ccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACt                                                                          <  2:521813/151‑1 (MQ=255)                                                                                  aaCGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGATTGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACt                                                                  >  2:40585/1‑150 (MQ=255)                                                                                        tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtctc                                                            <  1:119645/150‑1 (MQ=255)                                                                                        tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcg                                                           >  1:425869/1‑151 (MQ=255)                                                                                        tAAATGATGCGTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtctc                                                            <  2:459261/150‑1 (MQ=255)                                                                                           atgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc                                                        <  1:665354/151‑1 (MQ=255)                                                                                           atgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc                                                        <  1:339525/151‑1 (MQ=255)                                                                                            tgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgcg                                                       >  2:576511/1‑151 (MQ=255)                                                                                              atgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGa                                                      >  1:55313/1‑150 (MQ=255)                                                                                                 cTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGc                                                  >  1:408061/1‑151 (MQ=255)                                                                                                     aTACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCg                                              >  2:966/1‑151 (MQ=255)                                                                                                       aCAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTc                                            >  2:445475/1‑151 (MQ=255)                                                                                                       aCAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTc                                            <  1:214119/151‑1 (MQ=255)                                                                                                                 taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGc                                   >  2:189339/1‑150 (MQ=255)                                                                                                                  aaaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCAt                                 <  1:363425/151‑1 (MQ=255)                                                                                                                      aGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCAtttt                              >  1:138931/1‑150 (MQ=255)                                                                                                                      aGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTa                             <  1:558586/151‑1 (MQ=255)                                                                                                                       gAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTAt                            >  2:192160/1‑151 (MQ=255)                                                                                                                           gAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGcc                         <  1:480589/150‑1 (MQ=255)                                                                                                                           gAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCg                        >  2:174678/1‑151 (MQ=255)                                                                                                                                          gTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGTTACTaaaa         <  2:467455/151‑1 (MQ=255)                                                                                                                                             tGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGTTACTAAAAAGt      >  1:467184/1‑151 (MQ=255)                                                                                                                                                 catcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGTTACTAAAAAGTCAGt  >  1:364580/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                ACGATCTTCATCCAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑AAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGTTACTAAAAAGTCAGT  >  NC_000913/4494726‑4495019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑