Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	702,597	(G)5→6	coding (155/1149 nt)	nagA ←	N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	702,592	1	.	G	100.0%	131.8 / NA	37	G54R (GGT→CGT)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (19/18);  total (19/18)

CGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACC‑GGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCT  >  NC_000913/702442‑702735                                                                                                                                                        |                                                                                                                                                cGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAAcc                                                                                                                                                  <  1:287515/151‑1 (MQ=255)     ggtggtGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACC‑gggg                                                                                                                                             <  1:676910/151‑1 (MQ=255)              aaGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAAt                                                                                                                                     >  1:215332/1‑151 (MQ=255)                     ggCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCg                                                                                                                              <  2:464822/151‑1 (MQ=255)                        aGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTc                                                                                                                           >  2:97451/1‑151 (MQ=255)                         gATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCa                                                                                                                          <  2:499303/151‑1 (MQ=255)                           tagttagtACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGt                                                                                                                        >  1:3987/1‑151 (MQ=255)                                agtACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACg                                                                                                                   <  1:334483/151‑1 (MQ=255)                                 gtACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACgt                                                                                                                  <  1:70989/151‑1 (MQ=255)                                     aGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACgttgtt                                                                                                              >  1:361416/1‑151 (MQ=255)                                       ccTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCg                                                                                                            >  2:175138/1‑151 (MQ=255)                                            tttCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGAtctc                                                                                                       >  2:459567/1‑151 (MQ=255)                                               ctcATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTg                                                                                                     >  2:178333/1‑150 (MQ=255)                                                tcATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTggc                                                                                                   >  1:563152/1‑151 (MQ=255)                                                         ttCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTcc                                                                                          >  2:640435/1‑151 (MQ=255)                                                          tCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTcc                                                                                          <  2:74984/150‑1 (MQ=255)                                                                tGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCg                                                                                   >  2:555958/1‑151 (MQ=255)                                                                  aTTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCgg                                                                                  >  1:325889/1‑150 (MQ=255)                                                                  aTTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGa                                                                                 <  1:213125/151‑1 (MQ=255)                                                                  aTTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGa                                                                                 <  2:369957/151‑1 (MQ=255)                                                                      ccAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAg                                                                              <  2:92941/150‑1 (MQ=255)                                                                       cAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGAc                                                                            <  2:194683/151‑1 (MQ=255)                                                                         gCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGc                                                                          <  2:179754/151‑1 (MQ=255)                                                                           gTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCtt                                                                        <  2:438861/151‑1 (MQ=255)                                                                               ccACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTa                                                                     <  2:398886/150‑1 (MQ=255)                                                                                        ccGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCAt                                                           <  2:83019/151‑1 (MQ=255)                                                                                                    tgtCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATaaca                                                <  1:39705/150‑1 (MQ=255)                                                                                                        gTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACcgc                                           <  1:325905/151‑1 (MQ=255)                                                                                                          tAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGt                                         >  2:256996/1‑151 (MQ=255)                                                                                                             aCTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGt                                      >  1:469756/1‑151 (MQ=255)                                                                                                                  aCGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGtcatc                                  <  2:361406/150‑1 (MQ=255)                                                                                                                     ccgccgcAGCCGTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGtcatca                                 >  1:566399/1‑148 (MQ=255)                                                                                                                                gTTTAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACACCCGCGTGGTCATCAAGAAATTCGTGGcc                   <  1:606776/151‑1 (MQ=255)                                                                                                                                   tAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGt                >  2:336829/1‑151 (MQ=255)                                                                                                                                   tAACTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGt                >  2:51437/1‑151 (MQ=255)                                                                                                                                    aaCTGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGt                >  1:395428/1‑150 (MQ=255)                                                                                                                                       tGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTaa              >  1:575260/1‑149 (MQ=255)                                                                                                                                       tGCACATCGATAAAACCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTaa              >  1:647817/1‑149 (MQ=255)                                                                                                                                                  aaaaCCGGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCt  <  2:26925/150‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                CGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACC‑GGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCT  >  NC_000913/702442‑702735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑