Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,124,108	(G)6→7	intergenic (‑54/+10)	grxB ← / ← mdtH	glutaredoxin 2 (Grx2)/multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,124,102	1	.	G	100.0%	127.7 / NA	35	intergenic (‑48/+16)	grxB/mdtH	glutaredoxin 2 (Grx2)/multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (16/19);  total (16/19)

CGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCA‑GGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACGGAAGCTCTGGCTGGTGCGCCGATTT  >  NC_000913/1123955‑1124246                                                                                                                                                     |                                                                                                                                                 cgtTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCA‑ggg                                                                                                                                               >  1:167642/1‑151 (MQ=255)  gtTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCA‑gggg                                                                                                                                              >  2:577854/1‑151 (MQ=255)  gtTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCA‑gggg                                                                                                                                              >  2:356175/1‑151 (MQ=255)  gtTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCA‑gggg                                                                                                                                              >  1:679212/1‑151 (MQ=255)             cATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCaa                                                                                                                                    <  2:122183/151‑1 (MQ=255)                     tCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCg                                                                                                                            >  1:322027/1‑151 (MQ=255)                         cGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCAGTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTcgc                                                                                                                        >  1:552360/1‑151 (MQ=255)                          ggggATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGGTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTcgcg                                                                                                                       >  2:8982/1‑151 (MQ=255)                          ggggATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTcgcg                                                                                                                       <  2:323741/151‑1 (MQ=255)                                  ttttCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGc                                                                                                                <  2:18050/150‑1 (MQ=255)                                   tttCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCa                                                                                                               <  2:160596/150‑1 (MQ=255)                                    ttCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCaa                                                                                                              <  1:154363/150‑1 (MQ=255)                                        ggCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAacgac                                                                                                         <  2:664786/151‑1 (MQ=255)                                         gCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAacgacg                                                                                                        >  1:203895/1‑151 (MQ=255)                                               aaTCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACgcgcggc                                                                                                  <  1:589977/151‑1 (MQ=255)                                                   aTGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGt                                                                                              <  2:646736/151‑1 (MQ=255)                                                       gggCTTTGAAGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCt                                                                                          >  1:200497/1‑151 (MQ=255)                                                            ttGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCt                                                                                      >  2:72169/1‑150 (MQ=255)                                                             tGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTa                                                                                     >  1:65488/1‑150 (MQ=255)                                                              gAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTaa                                                                                    <  2:481998/150‑1 (MQ=255)                                                               aGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAAc                                                                                  <  2:119641/151‑1 (MQ=255)                                                                    gTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCa                                                                             <  1:378844/151‑1 (MQ=255)                                                                         ggCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCa                                                                        <  1:647033/151‑1 (MQ=255)                                                                           cAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAgc                                                                      <  2:492022/151‑1 (MQ=255)                                                                              tGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAgcgc                                                                    >  1:594213/1‑150 (MQ=255)                                                                                 tCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAgg                                                                >  2:323394/1‑151 (MQ=255)                                                                                        tGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTg                                                          >  2:134623/1‑150 (MQ=255)                                                                                         gTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGaa                                                        <  2:395492/151‑1 (MQ=255)                                                                                             aGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGAt                                                     <  2:560376/150‑1 (MQ=255)                                                                                                ttCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGcc                                                  >  2:252554/1‑150 (MQ=255)                                                                                                   aCGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCaata                                              <  2:203890/151‑1 (MQ=255)                                                                                                       gACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATg                                           <  1:446757/150‑1 (MQ=255)                                                                                                       gACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGc                                          >  1:188782/1‑151 (MQ=255)                                                                                                                 tttGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGcatcat                                >  2:481230/1‑151 (MQ=255)                                                                                                                        gTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACgg                          <  2:104522/150‑1 (MQ=255)                                                                                                                             ttCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACGGAAGctc                    >  1:225945/1‑151 (MQ=255)                                                                                                                                aCTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACGGAAGCTctgg                 <  2:4084/151‑1 (MQ=255)                                                                                                                                     cAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACGGAAGCTctggctg              >  2:98383/1‑149 (MQ=255)                                                                                                                                               gAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACGGAAGCTCTGGCTGGTGCGCCGAttt  >  2:338102/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCA‑GGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACGGAAGCTCTGGCTGGTGCGCCGATTT  >  NC_000913/1123955‑1124246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑