| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 907,602 | 0 | A | C | 55.9% | 35.4 / 50.2 | 34 | G227G (GGT→GGG) | ybjT | putative NAD‑dependent oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (12/3); new base C (0/19); total (12/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.30e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-07 | |||||||||||
GCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAG‑AAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGG > NC_000913/907457‑907747 | gcgcGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAG‑AAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGc < 2:472123/150‑1 (MQ=255) tcatcCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAG‑AAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGc < 2:411059/149‑1 (MQ=255) ccGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAG‑AAACCACACCGAAATCCAGCGGGGGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATaa > 1:6239/1‑150 (MQ=255) agttCGTGTTTCAGCCCCTAAATCACCGCCCGGGGGGTGGGGGGGGGTAGGGAAGTAATCACATTGAG‑AACCCACACCGAAACCCGGGGGGTGGGGAGGGGGAGGGGGATCACCCAGCGGCGCTTCCCCCTCACCGCCATAAAATg < 2:480819/143‑1 (MQ=255) agATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAG‑AAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCa > 2:101265/1‑150 (MQ=255) agATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAG‑AAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCa > 1:301247/1‑150 (MQ=255) ttgcAGCCCCTGAATCAACGCCCGGGGGGGGGGGGGCGGTAGGGAAGAAATCACATTGAG‑AACCCACACCAAAACCCGGGGGGGGGGGAGGGGGTGGGGGATCAACGGGGGGCGCTTCCCGCTAACCGCCATAAAATGTTCAAACTGTTg < 2:392476/147‑1 (MQ=255) ccTGAATCAACCCCCTGGCGGGGGGGGGGGGTACGAAAGTAATCACATTGAA‑AAACCACGCCAAAACCCAGCGGGTGGGGAGGGGGAGGGGAATCACCGAGCGGTGCTTCCCTCCAACCGCCATAAAATGTTCAAACTGTTGCTGATAACt < 1:41909/151‑1 (MQ=255) cgaaagaaccccccGGGGGGTGGGGGGGGTTACGAAAGAAACCA‑AATGAGAAAACCCCCCCAAAATCCAGCGGGTGGGGGGGGGGAGGGGGACCACCCAGCGGCGCTTCCCTCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAAc < 1:522398/138‑1 (MQ=255) tCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAG‑AAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCAc > 1:507720/1‑151 (MQ=255) gggggggggggggggTAGGGAAGAAATCACATTGAA‑ACCCCACCCCAAAACCCGGGGGGGGGGGAGGGGGAGGGGAATCAACCAGCGGCGCTTCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcg < 2:147297/142‑1 (MQ=255) gggggggggggggggTACGGAAGTAATCACATTGAA‑AACCCACCCCGAAACCCGGGGGGGGGGGGGGGGGAGGGGGATCAACAAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTcc < 1:620353/141‑1 (MQ=255) ggggggggggggTAGGAAAGTAATCACATTGAA‑AACCCCCCCCAAACCCCGGGGGGGGGGGAGGGGGTGGGGAATCACCGAGGGGCTCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggc < 2:196425/144‑1 (MQ=255) tAATCACATTGAG‑AAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTc > 1:311033/1‑151 (MQ=255) cacaTTGAG‑AAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTg > 2:422019/1‑151 (MQ=255) acaTTGAA‑AAACCACCCCAAAACCCGGGGGGTGGGGGGGGGGAGGGGAATAAACCAGGGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCt < 1:610370/149‑1 (MQ=255) ttGAG‑AAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCg > 1:46803/1‑151 (MQ=255) aaaCCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGgatg > 1:194142/1‑150 (MQ=255) acccacccgaaaaCCCAGGGGGTGGGGGGGGGGAGGGGAATCAACCGGCGGCGCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGgatga < 2:242094/140‑1 (MQ=255) aaaCCCGGCGGGGGGGGGGGGGGAGGGGAACCACCCAGGGGCGCTTCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGc < 1:5332/151‑1 (MQ=255) cccggCGGGGGGGGAGGGGGGGGGGAATCAACCAGCGGCGTTTCCCCCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAAc < 2:6239/147‑1 (MQ=255) cAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGCTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAAcc > 2:389594/1‑150 (MQ=255) cGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAAcc > 2:648005/1‑147 (MQ=255) gggggggggggggAGGGGGAACACCCGGCGGCGCTTACCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAg < 1:205205/148‑1 (MQ=255) ggggggggggggATGGGGAACACGCACCGGCTTTCCCCCCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGt < 1:60276/149‑1 (MQ=255) ggggatggggatCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTgcagca > 1:310670/1‑149 (MQ=255) ggggggggacAAACCAGCGGCGCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGtt < 2:137247/147‑1 (MQ=255) gggaatCACCCAGCGCCTCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTcc < 1:368968/151‑1 (MQ=255) ggaatCAACCAGGGGCCCTTCCCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCa < 2:568660/148‑1 (MQ=255) gaaccacccAGGGGCCTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAg < 1:621245/144‑1 (MQ=255) cAACCAGCGGCCCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAgcg < 1:658343/149‑1 (MQ=255) gcggcgCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGc < 1:505462/151‑1 (MQ=255) gcgcTTCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATggg < 2:281069/147‑1 (MQ=255) cgcTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCgtgg > 1:267712/1‑151 (MQ=255) | GCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAG‑AAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGG > NC_000913/907457‑907747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |