| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,842,914 | 0 | A | G | 46.9% | 23.5 / 10.4 | 32 | M182V (ATG→GTG) | gdhA | glutamate dehydrogenase, NADP‑specific |
| Reads supporting (aligned to +/- strand): ref base A (1/16); new base G (15/0); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.66e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGA > NC_000913/1842764‑1843062 | gATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGga < 1:476511/151‑1 (MQ=255)gATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTCTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGga < 1:182388/151‑1 (MQ=255) ttCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGgat‑g < 2:490421/150‑1 (MQ=255) cGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCAACTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGA‑GATGaaaa > 1:611617/1‑150 (MQ=255) aGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGT‑GACGaaaaa > 1:486405/1‑147 (MQ=255) aaaaGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGT‑GTTGgaaaaaagctcc > 2:387952/1‑143 (MQ=255) aaaaGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGT‑GGTGGAAAAAATCTcc > 1:371767/1‑150 (MQ=255) aaaaGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCca < 1:288101/151‑1 (MQ=255) gAAGGTGAAGTGCTGCGATTGTTCCAGGCGCTGATGACTGAAATGTATCGCCACCTGGGCGCGGATACCGACGTTTCGGCAGGTGATATCGAGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTATCcaacaaga < 2:633279/151‑3 (MQ=255) gAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGG‑GATGGAAAAAATTTCcaaaca > 2:102121/1‑146 (MQ=255) aaGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGG‑GATGAAAAAGCTCTCcaacaaaa > 1:516841/1‑148 (MQ=255) aaGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATAc < 1:521713/151‑1 (MQ=255) atgaCTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGGTTGAAAAAAACTACCAAAAAAACCCCCTGG‑GTC‑TTACCCCGGAGGGGCCttcat > 2:333053/1‑148 (MQ=255) tgaCTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCAt < 1:580087/150‑1 (MQ=255) gaCTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCAttt < 2:334992/151‑1 (MQ=255) gAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTggcg < 2:599595/151‑1 (MQ=255) aaCTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGTTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTggcg < 1:594772/150‑1 (MQ=255) aCTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAG‑GATTAAAAAAACCTCCACCAAAACCGCCCGC‑GGC‑TTCACCGGGAAGGGCCCTTTAtttttcggg > 1:256389/1‑145 (MQ=255) tCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGT‑GATGAAAAAACTCTCCAACAATACCGCCCGC‑GTC‑TTCACCGGGAAGGGGCTTTCCTTTTGCGGCCGTCtt > 2:594866/1‑151 (MQ=255) cGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCtt < 2:50697/150‑1 (MQ=255) tGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCg < 1:387082/148‑1 (MQ=255) gcgGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGaa < 1:557078/150‑1 (MQ=255) ccGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGG‑GGTGAAAAAGAACTCCAACAAAACCCCCCGC‑CTC‑TTTCCCCCGAAGGGCGTTTCATTTTTCGGCCGGCTTTTTTCCCCCGCAGCAAcc > 2:80216/1‑149 (MQ=255) cGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGT‑GATGAAAAAACTCTCCAAAAAAAACGCCCGC‑GTC‑TTTACCCGGAAGGGGCTTTTTTTTTGGGGCCGGCTTTTTTTCCCCGGAGCTAcccg > 1:40663/1‑148 (MQ=255) ccGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGt < 1:57782/150‑1 (MQ=255) ggCAGGTGATATCGGGGTGGGTGGTCGTGAAGCCGGCTTTAGGGCGGGGAT‑GATGAAAAACCTCCCCAACAATTCCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTATgg < 2:448700/147‑1 (MQ=255) gTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGT‑GGTGGAAAAAATCTCCCACAAAACCCCCCGC‑GTC‑TTCTCCCGTAAGGGCCTTTCTTTTTGCGGCGGTCTTATTTTCCCCGAAGCTACCGGCCACGGGCTCGttt > 1:62184/1‑150 (MQ=255) gATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGT‑GATGAAAAAACTCTCCAACAAAAACCCCTTC‑GTC‑TTTACCCGTTAGGGCCTTTTCTTTTGCGGGCGTCTTTTTCTCCCCGCAGCCACCCGCCACCGTCTGGGTtttt > 1:639915/1‑151 (MQ=255) atatCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGT‑GATGAAAAAAATCTCCCACAAAACCGCCTGC‑GGC‑TTCACCCGGAAGGGGCCTTTATTTTGCGGGCGGCTTTTTTTCCCCGGAGCTACCCGCCACGGGCTGGGtttttt > 1:501881/1‑151 (MQ=255) tggtggTCGTGAAGTCGGCTTTATGGCGGGGGG‑GATGGAAAAAATCTCCCACAAAAACCCCCGC‑GTCTTTAACCGGAAAGGGCCTTT‑TTTTTGCGGCAGGCTTTTTTTCCCCGAAGGCACCGGCCACGGTCTTGTttttttttccaaa > 2:507411/1‑141 (MQ=255) ggtCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGc < 1:458764/151‑1 (MQ=255) gtCGTGAAGTCGGCTTTATGGCGGGGGG‑GGTGAAAAAAATCTCCCACAAAACCCCCCGCGGCC‑TCCCCCGGAAAGGGCCTTTC‑TTTTGCGGCCGTCTTATTTCCCCCGGAGCTACCGGGTACCGGCTGGGTTATTTTTCAAAAGCaa > 1:42242/1‑147 (MQ=255) gTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGcc < 1:440292/151‑1 (MQ=255) cGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCAc < 2:607016/151‑1 (MQ=255) aTGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATg < 2:469522/150‑1 (MQ=255) at‑gatgAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGa > 1:84006/1‑149 (MQ=255) | GATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGAT‑GATGAAAAAGCTCTCCAACAATACCGCCTGC‑GTC‑TTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGA > NC_000913/1842764‑1843062 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |