| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,265,617 | 0 | C | A | 43.8% | 51.7 / 13.6 | 34 | F56L (TTC→TTA) | yeiP | elongation factor P‑like protein |
| Reads supporting (aligned to +/- strand): ref base C (17/1); new base A (0/14); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.18e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGTATGGTACTGAATTACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGT > NC_000913/2265476‑2265765 | aGGTATGGTACTGAATTACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGtgat > 2:39098/1‑151 (MQ=255) aTGGTACTGAATTACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTgatga‑t > 2:58793/1‑150 (MQ=255) gTACTGAATTACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCg > 2:554615/1‑151 (MQ=255) gAATTACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGac > 2:307044/1‑151 (MQ=255) aaTTACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGac > 2:331209/1‑150 (MQ=255) aaTTACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGac > 2:130244/1‑150 (MQ=255) aCGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGAc < 2:477361/151‑1 (MQ=255) ggCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGACGc > 1:204271/1‑151 (MQ=255) tgctgctgGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGACGCTGACccg > 2:232117/1‑151 (MQ=255) tgGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGCGCCGATACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGACGCTGACccgccg > 1:619578/1‑148 (MQ=255) atattCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTGACTTCTCCt > 2:64523/1‑150 (MQ=255) atattCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTGACTTCTCCt > 2:106749/1‑150 (MQ=255) ccccccCTGCCCGCGGTGCAGCTACGCTGAATAAAGGGCTTTTTTGTAATGCCG‑GTCCCGGGATAAAAGAAGAAGGGTGTTAAAAGGGTGATAA‑TATTGTTGACCCGGGGCCGCTCCCCCGCCGTTACGTTGACTTCTCCTATGTCg < 2:429078/145‑1 (MQ=255) cGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATgg > 1:52980/1‑151 (MQ=255) cGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATgg > 1:86373/1‑151 (MQ=255) gCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGc > 2:30546/1‑151 (MQ=255) ccccCTGCCGGGGGTGCCGCTACGTTGAAAAAATTGTTTTTTTCTGAGGCCC‑GCGCCGGGCAGAAAGTAGAGGTGTGTTAAAAGGGTGATGT‑TATCGTTGCCACCGTGACGCTGCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCa < 2:435023/151‑1 (MQ=255) cccGCTGCCCGCGGGCCCGCTCCGTTATAAAAAATGCGTTTTT‑TGGTGGCCGGTGCGGGGTTAAAAGAGGAGGTGTTTTAAAAGGGTGATGA‑TGGTGTTGACGCGGGGCCGCTGAGCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCa < 1:24514/151‑1 (MQ=255) cccctcccgggggTGCCGCTAGGTTAAAAAAATTGTTTTTTTCTGATGTCC‑GTCCGGGGCAAAAAGTAAAAGGGTTTTAAAAAGGTGATGA‑TATCTTTGACCCCGTGACGCTGCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCaa < 1:673585/140‑1 (MQ=255) cccgccccggggccccGCTACGGTAAAAAAATTGTTTTTTTTTTATGCCC‑GCCCCGGGATAAAAGAAGAGGTGTTTTTAAAGGGTAATTA‑TTTCTTTCACCCCGGCCCCCTCACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAAc < 2:304842/137‑1 (MQ=255) ggggTGCCGCTACGCTGTAAAAAATGCTTTTTTCTGATGCCC‑GTCCGGGGCTAAAAGAAGAAGAGCTTTAAAAAGGTGATGA‑TATCGTTGACCCCGTGACGCTGCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATg < 1:297156/148‑1 (MQ=255) gtcccGCTGCGCTGTAAAAAATGCTTTTTTCTGAGGTCC‑GTCCGGGGCTAAAAGAAGAAGAGCTTTTAAAGGGTATTGT‑TATCGTTGCCCCCGTGACGCGGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCt < 1:470361/147‑1 (MQ=255) ccccTACGTTGTAAAAATTGTTTTTTTCGTATGCCC‑GTCCGGGGCAAAAAGTAGAAGAG‑GTTTTAAAGGTTTTTATTTTCTTTGCCCCCGGGACCCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTa < 2:494573/147‑1 (MQ=255) ccGCTACGCTGAACAAAGTGTTTTTTTCTGATGTCC‑GTGGCGGGATAAAAGGAGAAGAGTTTTTAAAAGTTGATGA‑TTTCTTTGACCCCGGGACCGTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCt < 2:350371/147‑1 (MQ=255) gCTGTAAAAAATGCTTTTTTCTGATGTCC‑GTCCCGGGCAAAAAGTAGAAGAGCTTTTAAAGGGTGATGA‑TTTGGTGGACACGGGGAGGCTGCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGa < 1:671834/147‑1 (MQ=255) aaaaagTGTGTTTTTCTGTTGTCC‑GTCCCGGGTAGAAAGTAGAAGGGTGTTAAAAGGGTAATAT‑TTTCTTAGACCCGGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAaaga < 1:296951/146‑1 (MQ=255) gTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATAcccc > 2:574046/1‑151 (MQ=255) ttggATGCCC‑GCCCCGGGCTAAAAGTAGAAGAGTGTCAAAAGGGTGAGTA‑TTTCGTCGACCGGGTGACGCTGTCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGtat < 2:353219/147‑1 (MQ=255) cTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATAc > 1:14070/1‑151 (MQ=255) tgccc‑GTCCGGGACTGAAAAAAGAAGAGCTTTTTAAGGTTGATGA‑TTTTGTTAACACGGCGACGCTGCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCt < 2:66677/146‑1 (MQ=255) ccggccggggataaaaGTAGAAGAGCGTTTAAAGGGTGATGT‑TTTCGTTGCCCCGGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCt < 1:225717/133‑1 (MQ=255) cggggagaaaaGTAGAAGAGCTTTTAAAGGGTGATGT‑TTTGTTTACCCCCGTGCCGCTGCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCAc < 2:669845/137‑1 (MQ=255) aaaGAAGAAGAGCTTTAAAAAGGTGATTA‑TATCGTTGACCCGGTGACGCTGCCCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGa < 1:74507/148‑1 (MQ=255) ttCAAAGGTGATGA‑TATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGt > 2:679190/1‑151 (MQ=255) | AGGTATGGTACTGAATTACAACGGCAAACTGCTGCTGGTTAAGGATATTGATATTCAGTCGCCCACTGCCCGCGGTGCCGCTACGCTGTACAAAATGCGTTTTTCTGATGTCC‑GTACCGGGCTGAAAGTAGAAGAGCGTTTCAAAGGTGATGA‑TATCGTTGACACCGTGACGCTGACCCGCCGTTACGTTGACTTCTCCTATGTCGATGGCAACGAATATGTCTTTATGGATAAAGAAGACTATACCCCGTATACCTTCACCAAAGATCAGATTGAAGAAGAGTTGCTGT > NC_000913/2265476‑2265765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |