Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,624,5080AT47.1% 34.9 / 19.7 34N489Y (AAC→TAC) ppkpolyphosphate kinase, component of RNA degradosome
Reads supporting (aligned to +/- strand):  ref base A (1/17);  new base T (16/0);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.54e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATA  >  NC_000913/2624363‑2624658
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gATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACttt                                                                                                                                                   >  2:97815/1‑151 (MQ=255)
gATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACttt                                                                                                                                                   >  2:372950/1‑151 (MQ=255)
 aTTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTAAttt                                                                                                                                                   >  1:652390/1‑150 (MQ=255)
    tCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACttttttt                                                                                                                                               >  1:87209/1‑150 (MQ=255)
        gTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGaaaa                                                                                                                                           <  1:512815/151‑1 (MQ=255)
              aaaaCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATa                                                                                                                                     <  2:555569/151‑1 (MQ=255)
              aaaaCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTGAAAAacc                                                                                                                                        >  1:548202/1‑148 (MQ=255)
                 aCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCg                                                                                                                                  <  1:380105/151‑1 (MQ=255)
                 aCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTATTGAAAAACCATAcc                                                                                                                                   >  1:533397/1‑150 (MQ=255)
                    gTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGTTTTTACTTTTTTTTAAAACCATACCCTcc                                                                                                                               >  2:41062/1‑151 (MQ=255)
                           ggtgCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACa                                                                                                                        <  1:330083/151‑1 (MQ=255)
                             tgCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTATTTAAAAACCATACCGTCCGGTGACAtt                                                                                                                      >  1:671241/1‑151 (MQ=255)
                              gCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACAttt                                                                                                                     <  2:347068/151‑1 (MQ=255)
                              gCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACAtt                                                                                                                      <  1:330157/150‑1 (MQ=255)
                                         acaTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAAt                                                                                                          <  1:487511/151‑1 (MQ=255)
                                          caTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTACTTTTTTTTAAAACCATACCCTCCGGGGACATTTGATTTTTTTAt                                                                                                          >  2:388362/1‑150 (MQ=255)
                                             cGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGtg                                                                                                      <  1:378716/151‑2 (MQ=255)
                                                gACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTTAAAACCCATACCCTCCGGGGACATTTGTTTTTTTAAttggat                                                                                                     >  2:622634/1‑144 (MQ=255)
                                                        aCTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTATTTAAAAACCCTACCCTCCGGGGGCATTTTATTTTTTTATGGTATTGCCGCaaa                                                                                            >  2:406643/1‑150 (MQ=255)
                                                                gAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTAATTTTTTTAAAACCCAAACCCTCCGGGGACATTTTATTTTTTATTTGTTTTGCCCCaaaaaacccccc                                                                                   >  2:299613/1‑143 (MQ=255)
                                                                     aaCCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTACTTTTTTTAAAACCCCCACCCCCCCGGGGCATTTTGTTTTTTTATTGTATTGCCGCCAAACcccccccccc                                                                                >  2:557103/1‑149 (MQ=255)
                                                                        cgcgcgTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTGAAAACCCACACCCTCCCGGGGCATTTTGTTTTTTTTTTGTATCGCCGCAAAACTCCCcccgcccctt                                                                            >  2:637396/1‑146 (MQ=255)
                                                                          cgcgTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTa                                                                         <  2:419746/151‑1 (MQ=255)
                                                                           gcgTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTAt                                                                        <  1:153619/151‑1 (MQ=255)
                                                                            cgTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATg                                                                       <  1:493030/151‑1 (MQ=255)
                                                                                  tataCTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTTAAAAACCCTACCCTCCGGGGGCATTTTATTTTTTTATTGTATTGCCCCAAAAATCCCCCCGCCTATTTTATTaaa                                                                    >  1:60856/1‑148 (MQ=255)
                                                                                      cTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTAAATTTTTTTTAAAACCAAAACCTCCCGGGGCATTTTATTATTTTATGGGATCGCCGCCAAAACCCCCCCGCCCCTTGTATGTAAtggggg                                                              >  2:400479/1‑150 (MQ=255)
                                                                                           tATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGCATTTACTTTTTTTAAAACCCAAATCATCCCGGGACAATTGTTTATTTAATTGTGAGGCCCCAAAACCCCCCCCGCCTCTTTTATGGAATGGTGGatccc                                                         >  1:317430/1‑146 (MQ=255)
                                                                                                           ccgATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca                                        <  1:97817/151‑1 (MQ=255)
                                                                                                                gcgcgcATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAg                                   <  1:298754/151‑1 (MQ=255)
                                                                                                                        cACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGccc                           <  1:81414/151‑1 (MQ=255)
                                                                                                                                    aCGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCAcc               <  1:660402/151‑1 (MQ=255)
                                                                                                                                               ttAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTaa     <  2:30110/150‑1 (MQ=255)
                                                                                                                                                tAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTaaa    <  1:384240/150‑1 (MQ=255)
                                                                                                                                                 aaCTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAAt   >  1:338474/1‑150 (MQ=255)
                                                                                                                                                 aaCTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATa  <  1:193519/151‑1 (MQ=255)
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GATTTCACGTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATA  >  NC_000913/2624363‑2624658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑