Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,757,6680CT46.7% 7.2 / 17.7 30A54T (GCA→ACA) yiaYL‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (6/10);  new base T (0/14);  total (6/24)
Fisher's exact test for biased strand distribution p-value = 1.85e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGTGGAGAACCACCGCCTAAGGAGATCACGCTATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAA  >  NC_000913/3757524‑3757805
                                                                                                                                                |                                                                                                                                         
cGTGTGGAGAACCACCGCCTAAGGAGATCACGCTATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTg                                                                                                                                     <  1:597805/151‑1 (MQ=255)
      gagaACCACCGCCTAAGGAGATCACGCTATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATc                                                                                                                               <  2:495841/151‑1 (MQ=255)
                      ggAGATCACGCTATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAAt                                                                                                               >  2:214701/1‑151 (MQ=255)
                            cACGCTATCGAATTTATTCTTTTTAAGTAATTTCAACCCGGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCAAAAAATTTTGCTTTCTTCCAGTGTTTTTTGCACACCGCCCGCCATACCTAATTTCGt                                                                                                          <  2:434343/150‑1 (MQ=255)
                                   tCGAAATTATTCTCTTTAAGTAATTCCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCAAAAAATATTGCTTTCTTCCATTGTTTTTTGCACACCGCCCGCCATACCTAATTTCGTTAACat                                                                                                    <  1:133548/149‑1 (MQ=255)
                                         ttattCTCTTTAATTAATTTCAAACCGGCGGCGCCGTTTTCGGTGTTGGGGTTAGTTTGGGTGCCTTCATAAATAACGCAAAAAATTTTGCTTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACat                                                                                                    <  2:164430/143‑1 (MQ=255)
                                          tattCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGt                                                                                           >  1:127650/1‑151 (MQ=255)
                                          tattCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGt                                                                                           >  2:162393/1‑151 (MQ=255)
                                              ctctTTAAGTAATTTCAACCCTGCGGCGACTTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATAATAAATACCTCTAAAAATATTGCTTTCTCCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGa                                                                                         <  2:76445/149‑1 (MQ=255)
                                                    aattAATTTCAACCCTGCGGCGACGTTTCCCGTGGTGGGTTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCTTTCTTCCAGTTTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAAtt                                                                                    <  1:329582/145‑1 (MQ=255)
                                                    aaGTATTTTAAAACCGGCGGCGACGTTTTCCGTGGTGGGGTTAGGTGGGGTGCCATCAAAAATAACGCTAAAAATATTGCGTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTaa                                                                                  <  2:402577/150‑1 (MQ=255)
                                                       tAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCAATCAAAAATAACGCTAAAATTATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGt                                                                               <  1:386906/150‑1 (MQ=255)
                                                              aaaCCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTa                                                                        >  1:393072/1‑150 (MQ=255)
                                                              aaaCCGGCGGCGACGTTTTCCGTGGTGGGGTTAGTTTGGGTGCCATCAAAAATAACGATAAAAATATTGCGTTCTTCCAGTTTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTa                                                                        <  2:148005/150‑1 (MQ=255)
                                                                        cggcGTTTCCCGTGGTGGGTTGAGGTTGGGTGCCATCATAAATACCGTTAAAAATATTGCGTTCTTCCGGTGTTTTTTGCACACCGTCCGCCATACCTAATTTCGTTAGCATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAAt                                                              <  1:563710/147‑1 (MQ=255)
                                                                          aCGTTTTCCGTGGTGGGTTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCt                                                            <  2:543251/150‑1 (MQ=255)
                                                                          aCGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCAAAAAATATTGCGTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCt                                                            <  2:54509/150‑1 (MQ=255)
                                                                             ttttCCGTGGGGGGGTTAGGTGGGGTGCCATCAAAAATAACACAAAAAATATTGCGTTTTTCCAGTTCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTg                                                           <  2:365318/148‑1 (MQ=255)
                                                                                         gggTTAGGTTGGGTGCCATAATAAATAACGCTAAAAATACTGCTTTTTTCCAGTGCTTTTTGCACATCCCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCa                                               <  2:176113/148‑1 (MQ=255)
                                                                                                  tGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTc                                    <  1:1422/150‑1 (MQ=255)
                                                                                                   gggTGCCATCATAAATACCGCAAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTc                                    <  1:10473/149‑1 (MQ=255)
                                                                                                   gggTGCCATAATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCa                                   <  1:120226/150‑1 (MQ=255)
                                                                                                        ccATAAAAAATAACGCAAAAAATATTGCGTTCTTCCAGTGTTTTTGGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAAt                             <  2:404280/151‑1 (MQ=255)
                                                                                                               aaataaCGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCCCATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGcc                      <  1:153793/151‑1 (MQ=255)
                                                                                                                 ataaCGCAAAAAATATTGCTTTCTCCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGa                    <  2:377605/151‑1 (MQ=255)
                                                                                                                       cTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACAt              >  2:430107/1‑151 (MQ=255)
                                                                                                                       cTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACAt              >  2:562096/1‑151 (MQ=255)
                                                                                                                                ttGCGTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGa       <  1:144375/149‑1 (MQ=255)
                                                                                                                                 tGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAg     <  1:132357/150‑1 (MQ=255)
                                                                                                                                        ttcCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGaa  <  2:447310/146‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                         
CGTGTGGAGAACCACCGCCTAAGGAGATCACGCTATCGCAATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAA  >  NC_000913/3757524‑3757805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑