Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,850,0070GA44.4% 19.2 / 15.4 27P44S (CCG→TCG) uhpBsensory histidine kinase in two‑component regulatory sytem with UhpA
Reads supporting (aligned to +/- strand):  ref base G (11/4);  new base A (0/12);  total (11/16)
Fisher's exact test for biased strand distribution p-value = 1.82e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGGCCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGG‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCATCAAACAT  >  NC_000913/3849862‑3850153
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attAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGGCCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGG‑AAATa                                                                                                                                               <  2:214236/149‑1 (MQ=255)
     aCTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGGCCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCGGACCAAACGG‑AAATAACAGc                                                                                                                                          >  2:657455/1‑151 (MQ=255)
               atcaATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGGCCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGG‑AAATAACAGCACCGcc                                                                                                                                    <  1:128769/147‑1 (MQ=255)
                                         gaccgCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGGCCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGG‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTg                                                                                                      >  2:296151/1‑151 (MQ=255)
                                          cccccgtGCTTTACCCAAAAAATCAGCACCCCCCCCGCCCCCAGCAATGGGGGCAAGTACCCGCGGGGGCTTTGAAGCATGAGCCCCAGACGAAGACAAAAGGA‑AAAGACCAGCACCGCCATATCAGGGCGCTCAACCAGATGCAgg                                                                                                         <  1:200278/141‑1 (MQ=255)
                                            cgCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGGCCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGG‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGAt                                                                                                    >  2:634409/1‑150 (MQ=255)
                                                    ttAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGGCCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGG‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCcac                                                                                             >  1:344269/1‑149 (MQ=255)
                                                                       cccccccGCGCCCAACAATCCGGCCCAGTCCCCGCGGGGACTTCTCGTCATAACCCCCAAACGCAAACCAACGGA‑AAAAAACAGCCCCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCagag                                                                        <  1:367785/146‑1 (MQ=255)
                                                                       ccACCCCGCCCCCAAAAAGACGGGCCAGTACCCGGGCGGACTTTGCAGAATTCGCCCCAGACGCA‑AAAAAACGGAAAATAACACCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCag                                                                          <  2:307316/149‑1 (MQ=255)
                                                                        cccccccccccccaaaaTCCGGGCCAGTACCCGCGGGGCCTGCCCAGCATTCCCCCCAGACCAAGAAAAAACGA‑AAAAAACGCCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAgaga                                                                       <  2:557017/136‑1 (MQ=255)
                                                                                 ccaaGCAATGCGGGCCAGTATCCGCGCGGGCATTGCAGCATTACCCCCAGACGCAAACAAAACGA‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATaa                                                                 <  1:338563/145‑1 (MQ=255)
                                                                                  ccAGCAATACGGGCCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGG‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATaaaaa                                                              >  2:288819/1‑150 (MQ=255)
                                                                                  acAGCAATACGGGCCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGG‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATaaaaa                                                              >  2:62332/2‑150 (MQ=255)
                                                                                      cAATACGGGCCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGG‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGc                                                           >  2:506635/1‑149 (MQ=255)
                                                                                       aacccGGCCCAGTACCCGCGCGGCCATTCCACCATTCCCCCCAGCCCCACCCAAAACAA‑AAAAAACACCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCa                                                          <  2:325091/145‑1 (MQ=255)
                                                                                       aaagggggCCAGTCCCCGGGGGGGCATGCCAGCATAGGCCCCAGACGCAAACAAAGGGG‑AAAAACCAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATaaaa                                                               <  2:483466/139‑1 (MQ=255)
                                                                                               ccactccccGCGGGGGCATTGCAGCATCACCCCCAGACGAAGACAAAAGAA‑AAAAAACACCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGc                                                       <  2:330845/137‑1 (MQ=255)
                                                                                               cagctacccGCGGGGCCTTTGCACCATCACCCCAAGCCGAAGACAAAAGGA‑AAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAAc                                                 <  2:110505/143‑1 (MQ=255)
                                                                                                            gggCATTCCACCATTGCCCCAAGACCCAGACAAAGCGA‑AAAAAACACGGCCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAg                                       <  2:443368/147‑1 (MQ=255)
                                                                                                            ggactttGCAACATCAGCCCAAGACGCAAACAAAGGGA‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTaa                                            <  1:330899/137‑1 (MQ=255)
                                                                                                                    cagcaTAACCCCCAGACGAAGACAAAAGGA‑AAAAAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGaaca                             <  2:320967/149‑1 (MQ=255)
                                                                                                                              cccAGACGCAGACCAAACGG‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTtcatc                  >  2:390981/1‑150 (MQ=255)
                                                                                                                               ccAGACGCAGACAAAACGA‑AAAAAACAGCCCCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTtca                    <  1:383984/147‑1 (MQ=255)
                                                                                                                                 aGACGCAGACCAAACGG‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCAcc               >  2:118847/1‑150 (MQ=255)
                                                                                                                                       aGACCAAACGG‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCATc        >  2:236142/1‑151 (MQ=255)
                                                                                                                                         aCCAAACAA‑AAAAAACACCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAg             <  2:334195/144‑1 (MQ=255)
                                                                                                                                             aaCGG‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCATCAAACAt  >  2:517751/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGTAAACTACCGATCATCAATAACGGAAAATGGGTTAAACCGACCGCCTGCGTTAGCCAGTAAATCAGCAGCCACTCCGCGCCCAGCAATACGGGCCAGTATCCGCGCGGGCATTGCAGCATTAGCCCCAGACGCAGACCAAACGG‑AAATAACAGCACCGCCATATCAGGGCGCTCAACCAGATGCAGGCTGATACTCCACAGGCAAAACCATGCGGCAGAGAAGATAAAAAAGCAGGCAATAACGGTAATTAAGCGGGAGAACAACGTCTTCATCACCAGCCATCAAACAT  >  NC_000913/3849862‑3850153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑