Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,991	C→T	A275V (GCG→GTG)	dnaJ →	chaperone Hsp40, DnaK co‑chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,991	0	C	T	100.0%	150.2 / NA	45	A275V (GCG→GTG)	dnaJ	chaperone Hsp40, DnaK co‑chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/25);  total (20/25)

CCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTG  >  NC_000913/14845‑15135                                                                                                                                                   |                                                                                                                                                 ccGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTg                                                                                                                                              <  2:134330/151‑1 (MQ=255)  cGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTgg                                                                                                                                             <  2:215827/151‑1 (MQ=255)  cGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTgg                                                                                                                                             >  1:36396/1‑151 (MQ=255)       tGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTg                                                                                                                                          <  2:545983/149‑1 (MQ=255)                                cATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATg                                                                                                               <  1:501665/151‑1 (MQ=255)                                cATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATg                                                                                                               <  2:174307/151‑1 (MQ=255)                                 aTGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGGGGCGAAATCGAAGTACCGACCCTTGATgg                                                                                                              >  1:185298/1‑151 (MQ=255)                                      gcACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGGGGGGAAATCGAAATACCCGCCCTTGATGGTcgc                                                                                                          >  2:601468/1‑150 (MQ=255)                                          cGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGGGGGGAAATCGAAGTACCGACCCCTGGTGGTCGCGTc                                                                                                       >  2:471721/1‑149 (MQ=255)                                                       gTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAg                                                                                             <  1:356367/146‑1 (MQ=255)                                                       gTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGGGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGcc                                                                                         >  1:420520/1‑150 (MQ=255)                                                                    caggttAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAAccc                                                                              >  1:643999/1‑148 (MQ=255)                                                                          aaaCAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGGGAAATCGAAGTACCGACCCTTGATGGTCGCGTTAAACTGAAAGTGGCTGGCGAAACCCAGACCGGt                                                                      >  2:345786/1‑150 (MQ=255)                                                                          aaaCAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGTCCGGTa                                                                     <  2:367119/151‑1 (MQ=255)                                                                                 aCCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTAtt                                                              <  1:402718/151‑1 (MQ=255)                                                                                   ccGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTcc                                                            <  1:6270/151‑1 (MQ=255)                                                                                    cGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCg                                                           <  1:361597/151‑1 (MQ=255)                                                                                    cGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCg                                                           <  1:295029/151‑1 (MQ=255)                                                                                    cGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGGGGGGAAAACGAAGTACCGACCCTTGATGGGCGCGTCAAACTGAAAGGGCCTGGCGAAACCCAGACCGGGAAGCTATTcc                                                            >  2:39066/1‑150 (MQ=255)                                                                                      aTTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAAACGAAGTACCGACCCCTGATGGGCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGAACGGTAAGCGATTCCGt                                                          >  2:205994/1‑150 (MQ=255)                                                                                           cGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATgcgc                                                    <  2:382031/151‑1 (MQ=255)                                                                                            gAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATgcgc                                                    >  1:556698/1‑150 (MQ=255)                                                                                            gAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGcgcg                                                   <  1:120855/151‑1 (MQ=255)                                                                                               cGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGGGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCgg                                                  >  1:139660/1‑149 (MQ=255)                                                                                                 tGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGGGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTaa                                               >  2:170911/1‑150 (MQ=255)                                                                                                    aGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGc                                           <  1:544678/151‑1 (MQ=255)                                                                                                     ggCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCg                                          <  2:636374/151‑1 (MQ=255)                                                                                                        aacaacCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTc                                        <  2:75807/150‑1 (MQ=255)                                                                                                         acaacCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCaa                                      <  2:444681/151‑1 (MQ=255)                                                                                                         acaacCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCa                                       >  2:370258/1‑150 (MQ=255)                                                                                                             ccTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCt                                  <  2:51924/151‑1 (MQ=255)                                                                                                               tGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGGGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAAATGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGGAAAGGCGTCAAGTCTGt                                >  2:16279/1‑151 (MQ=255)                                                                                                                gTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGt                                >  1:197347/1‑150 (MQ=255)                                                                                                                    tGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCcgc                            <  2:528125/150‑1 (MQ=255)                                                                                                                     gCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCTGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCgg                          <  2:651588/151‑1 (MQ=255)                                                                                                                      cGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGGGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAAATGAAAATGCCCGGCGAAACCCAGACCGGTAAGCTATTCCGGATGCGCGGTAAAGGCGTCAAGTCTGTCCGCgg                          >  2:382092/1‑150 (MQ=255)                                                                                                                       gAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGt                         <  2:94056/150‑1 (MQ=255)                                                                                                                       gAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGGGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAAGCGTCAAGTCTGTCCGCGGTg                        >  2:238086/1‑151 (MQ=255)                                                                                                                        aagatccGATCTACTTCGCTGTGGCTGTGCTGGGTGGCGAAATCGAAGTAGCGAACCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGAGAAACCCAGACCGGTAAGCTATTCCGTATTCGCGGTAAAGGCATCAAGTCTGTCCGCGGTgg                       <  2:332603/146‑1 (MQ=255)                                                                                                                         aGTCCCGATCAACTTCGCTATGGCGGTGCTGGGGGGCGAAATAGAAGTACCCACCCTTGATGGTCGCGTCAAACTGAAAGTGCCCGGCGGAACCCAGACCGGTAAGCTATTCCGTATGCGCGGGAAAGGCGTCAAGTCTGTCCGCgg                          >  2:539958/1‑147 (MQ=255)                                                                                                                              cGATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAATCCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCTGTGgcgc                    <  2:660597/148‑1 (MQ=255)                                                                                                                               gATCAACTTCGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAg                <  2:431918/151‑1 (MQ=255)                                                                                                                                   aaCTTCGCTATGGCGGTGCTGGGGGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGGAAAAGCGTCAAGTCTGTCCGCGGTGGCGCACAggggg            >  2:661178/1‑149 (MQ=255)                                                                                                                                        cGCTATGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAttt        <  1:516896/150‑1 (MQ=255)                                                                                                                                             tGGCGGTGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCtgtg  >  1:283556/1‑151 (MQ=255)                                                                                                                                              ggcggtGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCtgtg  <  1:31328/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCTGTG  >  NC_000913/14845‑15135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑