Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,175,182 A→G S380S (AGT→AGC)  gatZ ← D‑tagatose 1,6‑bisphosphate aldolase 2, subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,175,1820AG100.0% 137.7 / NA 39S380S (AGT→AGC) gatZD‑tagatose 1,6‑bisphosphate aldolase 2, subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/16);  total (23/16)

ACAGGTTAGTCATATACCGTCCTTATTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGACTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGTAATAACGAATACGATCCGACAGGCTGTAACGAATATCCAGTAATGAATCGTTAAA  >  NC_000913/2175037‑2175322
                                                                                                                                                 |                                                                                                                                            
acaGGTTAGTCATATACCGTCCTTATTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAAt                                                                                                                                         >  2:251546/1‑151 (MQ=255)
  aGGTTAGTCATATACCGTCCTTATTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCa                                                                                                                                       <  2:320875/151‑1 (MQ=255)
   ggTTAGTCATATACCGTCCTTATTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCAt                                                                                                                                      <  1:167579/151‑1 (MQ=255)
   ggTTAGTCATATACCGTCCTTATTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCAt                                                                                                                                      >  2:497321/1‑151 (MQ=255)
           atatACCGTCCTTATTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCa                                                                                                                                 <  2:102665/148‑1 (MQ=255)
               aCCGTCCTTATTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGa                                                                                                                           <  2:663999/150‑1 (MQ=255)
                 cGTCCTTATTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGt                                                                                                                        >  1:262617/1‑151 (MQ=255)
                    ccTTATTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCa                                                                                                                     <  2:120066/151‑1 (MQ=255)
                       tATTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGc                                                                                                                  >  2:429963/1‑151 (MQ=255)
                       tATTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGc                                                                                                                  >  1:481980/1‑151 (MQ=255)
                        aTTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGcc                                                                                                                 >  1:437800/1‑151 (MQ=255)
                        aTTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGc                                                                                                                  <  1:221334/150‑1 (MQ=255)
                        aTTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGc                                                                                                                  <  1:36437/150‑1 (MQ=255)
                              cacaGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAg                                                                                                           <  2:542977/151‑1 (MQ=255)
                               acaGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCa                                                                                                             >  2:74679/1‑148 (MQ=255)
                               acaGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCa                                                                                                             >  2:537503/1‑148 (MQ=255)
                                          gATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACcatcat                                                                                               >  1:444863/1‑151 (MQ=255)
                                           aTAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCatcatc                                                                                              <  1:341106/151‑1 (MQ=255)
                                              ggCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGtt                                                                                           >  1:583231/1‑151 (MQ=255)
                                                cgcgcAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTc                                                                                         <  2:616714/151‑1 (MQ=255)
                                                   gcAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGAc                                                                                      >  2:527933/1‑151 (MQ=255)
                                                         catcatAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTAtt                                                                                <  2:478339/151‑1 (MQ=255)
                                                            catAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTAttttt                                                                             >  1:118088/1‑151 (MQ=255)
                                                            catAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTAttttt                                                                             >  1:41208/1‑151 (MQ=255)
                                                                aaTTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATc                                                                         >  1:305926/1‑151 (MQ=255)
                                                                        ccATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTAt                                                                  <  1:135553/150‑1 (MQ=255)
                                                                                  cTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGta                                                        >  2:276162/1‑150 (MQ=255)
                                                                                   tGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGtaat                                                      >  1:216627/1‑151 (MQ=255)
                                                                                    gATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGtaat                                                      >  2:70193/1‑150 (MQ=255)
                                                                                    gATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGtaa                                                       <  2:249022/149‑1 (MQ=255)
                                                                                       gCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGTAATAACg                                                  <  2:252310/151‑1 (MQ=255)
                                                                                                        tCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGTAATAACGAATACGATCCGACAGGc                                 >  1:109623/1‑151 (MQ=255)
                                                                                                                 tGAATGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGTAATAACGAATACGATCCGACAGGCTGTAACGa                         >  2:277706/1‑150 (MQ=255)
                                                                                                                    aTGCGTTCAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGTAATAACGAATACGATCCGACAGGCTGTAACGAatat                     >  1:19641/1‑151 (MQ=255)
                                                                                                                           cAAATTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGTAATAACGAATACGATCCGACAGGCTGTAACGAATATCCAGTaa              <  1:158126/151‑1 (MQ=255)
                                                                                                                             aaTTGTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGTAATAACGAATACGATCCGACAGGCTGTAACGAATATCCAGTAATg            >  1:88427/1‑151 (MQ=255)
                                                                                                                                 gTTTGGGAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGTAATAACGAATACGATCCGACAGGCTGTAACGAATATCCAGTAATGAATc        <  2:655626/151‑1 (MQ=255)
                                                                                                                                      ggAAGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGTAATAACGAATACGATCCGACAGGCTGTAACGAATATCCAGTAATGAATCGTTa    >  1:449327/1‑150 (MQ=255)
                                                                                                                                        aaGATACTGGCTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGTAATAACGAATACGATCCGACAGGCTGTAACGAATATCCAGTAATGAATCGTTaaa  >  2:636785/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
ACAGGTTAGTCATATACCGTCCTTATTCCGCACAGCCGTAGCGATAGGCGCGCAAAACATCATAAATTTTATCCATAATCAGCTGATGCGGTATTGCTGATAATTCCCCGGACTGAATGCGTTCAAATTGTTTGGGAAGATACTGACTAATCATGCCCAGTGGGATGTCCACGCCTTCAAGATTCACCATCATCGTTTCGACGCTATTTTTAATCCGACTATGCGGCCAGTAATAACGAATACGATCCGACAGGCTGTAACGAATATCCAGTAATGAATCGTTAAA  >  NC_000913/2175037‑2175322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑