Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,223,301	T→C	F373L (TTC→CTC)	dld →	D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,223,301	0	T	C	100.0%	138.3 / NA	38	F373L (TTC→CTC)	dld	D‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

CACCTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGTTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGG  >  NC_000913/2223156‑2223434                                                                                                                                                  |                                                                                                                                      cacCTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTcccc                                                                                                                                  <  1:569008/151‑1 (MQ=255)  acCTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCa                                                                                                                                 <  1:334223/151‑1 (MQ=255)             gattgatAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTAccg                                                                                                                      <  2:314303/151‑1 (MQ=255)                tgatAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTAccgcc                                                                                                                    <  1:446888/150‑1 (MQ=255)                      gTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATg                                                                                                             >  2:543677/1‑151 (MQ=255)                           gCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGaaaa                                                                                                         >  1:460332/1‑150 (MQ=255)                            cACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAAc                                                                                                       >  2:305668/1‑151 (MQ=255)                             aCCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACt                                                                                                      <  1:582198/151‑1 (MQ=255)                                 aCAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGgcg                                                                                                  <  1:484583/151‑1 (MQ=255)                                     gATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGAt                                                                                              <  2:588331/151‑1 (MQ=255)                                        gCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATaaa                                                                                           <  2:406535/151‑1 (MQ=255)                                        gCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATaa                                                                                            >  1:369763/1‑150 (MQ=255)                                            ttcttcTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACg                                                                                       >  1:84158/1‑151 (MQ=255)                                               ttcttTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGc                                                                                    >  1:555996/1‑151 (MQ=255)                                                    tAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGcatcat                                                                               >  2:251489/1‑151 (MQ=255)                                                                 cGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTaaaaa                                                                  <  2:345133/151‑1 (MQ=255)                                                                   cACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATg                                                                >  2:381533/1‑151 (MQ=255)                                                                        atgcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcggg                                                           <  1:439330/151‑1 (MQ=255)                                                                          gcgctgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTAACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATgtcggg                                                           <  2:453244/149‑1 (MQ=255)                                                                                cTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCg                                                   >  1:114753/1‑151 (MQ=255)                                                                                cTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCg                                                   >  2:374857/1‑151 (MQ=255)                                                                                        aGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGaa                                           <  1:436820/151‑1 (MQ=255)                                                                                         gTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGa                                            >  1:83251/1‑149 (MQ=255)                                                                                         gTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGa                                            >  2:652717/1‑149 (MQ=255)                                                                                            aaaTTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGcc                                        >  1:292611/1‑150 (MQ=255)                                                                                              aTTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCaa                                      >  1:96062/1‑150 (MQ=255)                                                                                                   tccgtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATc                                   <  1:305675/148‑1 (MQ=255)                                                                                                    ccgtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGt                                 <  1:502583/149‑1 (MQ=255)                                                                                                    ccgtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTg                                <  2:105250/150‑1 (MQ=255)                                                                                                      gtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCt                             <  1:50404/151‑1 (MQ=255)                                                                                                           cATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggtgg                        <  1:626294/151‑1 (MQ=255)                                                                                                           cATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggtgg                        >  1:465929/1‑151 (MQ=255)                                                                                                               ttACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGatt                     <  2:542284/150‑1 (MQ=255)                                                                                                                 aCTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGattatt                  >  2:584423/1‑151 (MQ=255)                                                                                                                   tGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTAttt                 >  1:145474/1‑150 (MQ=255)                                                                                                                        gTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACa           <  1:548844/151‑1 (MQ=255)                                                                                                                           cGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCCAATCGTGGCTGGTGGATTATTTCAAACCgg         >  2:607177/1‑150 (MQ=255)                                                                                                                                  aaaaTTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAgg  >  1:439308/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                      CACCTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGTTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGG  >  NC_000913/2223156‑2223434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑