Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,261,739	T→C	A209A (GCA→GCG)	fruK ←	fructose‑1‑phosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,261,739	0	T	C	100.0%	148.7 / NA	40	A209A (GCA→GCG)	fruK	fructose‑1‑phosphate kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/18);  total (22/18)

CCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACA  >  NC_000913/2261605‑2261885                                                                                                                                       |                                                                                                                                                   ccTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACAt                                                                                                                                    >  1:629503/1‑151 (MQ=255)    gcgcCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATc                                                                                                                                   <  1:374287/149‑1 (MQ=255)      gcCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCtttc                                                                                                                               >  2:109370/1‑151 (MQ=255)      gcCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCtttc                                                                                                                               <  1:310773/151‑1 (MQ=255)        cAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCtttcat                                                                                                                             >  2:307257/1‑151 (MQ=255)          aCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCtttcattt                                                                                                                           >  2:452206/1‑151 (MQ=255)            ggTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCtttcatttca                                                                                                                         <  2:284414/151‑1 (MQ=255)                  tACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCa                                                                                                                     <  2:343869/149‑1 (MQ=255)                  tACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAg                                                                                                                    <  1:479867/150‑1 (MQ=255)                  tACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGt                                                                                                                   >  1:369342/1‑151 (MQ=255)                   aCGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGtt                                                                                                                  >  1:622276/1‑151 (MQ=255)                   aCGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGtt                                                                                                                  >  1:227218/1‑151 (MQ=255)                    cGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGttt                                                                                                                 >  1:445483/1‑151 (MQ=255)                       cATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACg                                                                                                              >  1:115828/1‑151 (MQ=255)                               gACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGcc                                                                                                       <  1:29457/150‑1 (MQ=255)                                        ttGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTcc                                                                                              <  1:223339/150‑1 (MQ=255)                                               tCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTcgcg                                                                                      >  1:622681/1‑151 (MQ=255)                                                    tCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGcggcgg                                                                                  <  2:154952/150‑1 (MQ=255)                                                     cGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGtt                                                                                <  1:229106/151‑1 (MQ=255)                                                       ccGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGtt                                                                                >  1:581590/1‑149 (MQ=255)                                                       ccGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAg                                                                              <  1:521150/151‑1 (MQ=255)                                                           aGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGttt                                                                          >  2:366058/1‑151 (MQ=255)                                                                     ccAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCa                                                                 >  1:160344/1‑150 (MQ=255)                                                                                cTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGGGCCGCttt                                                     >  1:290656/1‑151 (MQ=255)                                                                                  tCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCttt                                                     <  1:528422/149‑1 (MQ=255)                                                                                   cGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCaa                                                  <  2:425348/151‑1 (MQ=255)                                                                                      cACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAAcc                                               <  1:481691/151‑1 (MQ=255)                                                                                         ccAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGc                                            <  1:503308/151‑1 (MQ=255)                                                                                          cAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGct                                           <  2:365632/151‑1 (MQ=255)                                                                                            gTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGctac                                         <  1:495142/151‑1 (MQ=255)                                                                                                 aTAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTATGATTCACCACCCACCGGGCCGCTTTCACACCTGCTCCTaa                                      >  1:349111/1‑149 (MQ=255)                                                                                                   aacaacATGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCt                                  >  2:6722/1‑151 (MQ=255)                                                                                                    acaacaTGCGCGATGCATTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAAGTTTCACCAGGCAAGGGGGCGCTTTTAAAACTGCTTCCAACACtt                                 >  2:131134/1‑151 (MQ=255)                                                                                                        caTGCGCGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGGTTCACCAGCCACGGGGCCGCTTTCAAACCCGCTACTAACGCTTCa                               >  2:415104/1‑149 (MQ=255)                                                                                                           gcgcGATGCCTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGc                           <  2:316412/150‑1 (MQ=255)                                                                                                                   ccTTGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCCCCCGCCACGGTGCCGCTTTCAAACCTGGTACTAACGGTTCAACGctact                       >  1:86228/1‑146 (MQ=255)                                                                                                                      tGTTCACGTAGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAg                <  1:527347/150‑1 (MQ=255)                                                                                                                               aGCGCATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGGTTTACCAGCCACGGTGCCGCCTTCAAACCTGCTACTAAAGCTTCACGGCCACTATCAAAAATAATGCaa       >  1:302209/1‑150 (MQ=255)                                                                                                                                  gcATGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGCTAGGTTTCAACAACCACGGTGCCGCCTTCAAACCTGCTACTAACGCTTCCCGGACAACATTAAAGATAATTGAAgg     >  2:160540/1‑149 (MQ=255)                                                                                                                                    aTGCGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGGGCCGCTTTTCAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAAAGCAAAGaca  >  2:365227/1‑150 (MQ=255)                                                                                                                                       |                                                                                                                                                   CCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACA  >  NC_000913/2261605‑2261885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑