Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,269,424	T→C	W191R (TGG→CGG)	lpxT →	lipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,269,424	0	T	C	97.6%	140.9 / ‑6.8	42	W191R (TGG→CGG)	lpxT	lipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (28/13);  total (29/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAAC  >  NC_000913/2269283‑2269561                                                                                                                                              |                                                                                                                                          gaTAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTg                                                                                                                                  <  1:111497/151‑1 (MQ=255)    aGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGAc                                                                                                                                >  1:453181/1‑150 (MQ=255)                 aTCACGGCATGATGCTTCTTATTTTTTCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCACTTATTATTTTTGTGGATTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTAGGtt                                                                                                                   >  1:505645/1‑150 (MQ=255)                   cACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCg                                                                                                                 <  2:31257/150‑1 (MQ=255)                    aCGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGa                                                                                                                >  1:173399/1‑150 (MQ=255)                     cGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATCATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATg                                                                                                              >  1:185440/1‑151 (MQ=255)                          tgatgCTGCTTATTTTTTCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGt                                                                                                         >  2:15375/1‑151 (MQ=255)                           gatgCTGCTTATTTTTTCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTg                                                                                                        >  1:587756/1‑151 (MQ=255)                                         tttCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGttt                                                                                          >  2:474199/1‑151 (MQ=255)                                          ttCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTg                                                                                         >  2:260469/1‑151 (MQ=255)                                              gCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATAGATCGGTTTGCCCt                                                                                     <  1:185073/151‑1 (MQ=255)                                               cATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTg                                                                                    >  2:275942/1‑151 (MQ=255)                                                  tCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGgt                                                                                 >  1:479929/1‑151 (MQ=255)                                                      gtgGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTg                                                                             <  2:344835/151‑1 (MQ=255)                                                         gCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTg                                                                          >  1:562011/1‑151 (MQ=255)                                                                ttCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCa                                                                    <  2:653367/150‑1 (MQ=255)                                                                ttCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCa                                                                    <  1:114919/150‑1 (MQ=255)                                                                     cAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGt                                                              >  1:428279/1‑151 (MQ=255)                                                                          ttGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCg                                                         >  1:513073/1‑151 (MQ=255)                                                                              aGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTa                                                     <  2:357754/151‑1 (MQ=255)                                                                                 ccTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATc                                                  >  2:58262/1‑151 (MQ=255)                                                                                    aaTCGCCCTTATTAGTTTTGTGGGTTTTGGATGTGGCAGAGTAATGATTGGGGGACGCTGGTTTACTGACATCATTGTAGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGCGTTGCTGACGCCATTAAGTGATCGATTACTCAcc                                               >  2:129470/2‑151 (MQ=255)                                                                                       cGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCttt                                            >  1:352746/1‑151 (MQ=255)                                                                                        gCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCtttt                                           >  2:559370/1‑151 (MQ=255)                                                                                          ccTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCtttttt                                         <  2:292008/151‑1 (MQ=255)                                                                                             tattatTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTg                                        <  1:312721/149‑1 (MQ=255)                                                                                              attattTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACa                                     <  2:365752/151‑1 (MQ=255)                                                                                              attattTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGAGATCGATTAATCACCTTTTTTGAc                                      >  1:281198/1‑150 (MQ=255)                                                                                                tattTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACaa                                    >  2:472203/1‑150 (MQ=255)                                                                                                 attTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAAt                                  <  1:25406/151‑1 (MQ=255)                                                                                                 attTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAAt                                  >  2:504203/1‑151 (MQ=255)                                                                                                      tgtgGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACt                              >  2:193170/1‑150 (MQ=255)                                                                                                       gtgGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTa                             <  2:334712/150‑1 (MQ=255)                                                                                                        tgGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTAcc                           >  1:308623/1‑151 (MQ=255)                                                                                                           tttttGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGG‑TTGCCCTGGGTGGTGCTGACGCCATTAAGGGATCGATTAATCAACTTTTTTGACAAATCACTACCAgg                        >  1:256981/1‑150 (MQ=255)                                                                                                              ttGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGaa                      <  2:525240/150‑1 (MQ=255)                                                                                                              ttGCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGaa                      >  1:408686/1‑150 (MQ=255)                                                                                                                gCATTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGaaaa                    >  2:193362/1‑150 (MQ=255)                                                                                                                  aTTTCCCAGAGTAATGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGAAAaaac                 >  2:325109/1‑151 (MQ=255)                                                                                                                                tGATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCaaa    >  1:390630/1‑150 (MQ=255)                                                                                                                                 gATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAAc  >  2:463090/1‑151 (MQ=255)                                                                                                                                 gATTGGCGCACACCGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAAc  >  1:672407/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                          GATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAAC  >  NC_000913/2269283‑2269561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑