Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,325,637	T→C	R294R (CGT→CGC)	atoE →	short chain fatty acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,325,637	0	T	C	100.0%	140.8 / NA	41	R294R (CGT→CGC)	atoE	short chain fatty acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/20);  total (21/20)

TCTGACGTTGATCATCGGCGCACTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGTGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGACACCTTCCCG  >  NC_000913/2325490‑2325784                                                                                                                                                    |                                                                                                                                                    tCTGACGTTGATCATCGGCGCACTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATgcgcgc                                                                                                                                                   <  2:286914/150‑1 (MQ=255)    gACGTTGATCATCGGCGCACTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATc                                                                                                                                               <  2:502745/151‑1 (MQ=255)          gATCATCGGCGCACTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGcgcg                                                                                                                                         <  2:49812/151‑1 (MQ=255)               tcGGCGCACTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGgcagc                                                                                                                                    >  1:317300/1‑151 (MQ=255)                      aCTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGt                                                                                                                             <  2:223252/151‑1 (MQ=255)                        taggTATCGCTTACCTTGCGATGAACTTCAGCGAACATGGCTTCAACACCACCATCGATACAGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAATCGCCACCGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGt                                                                                                                             >  2:431370/3‑149 (MQ=255)                                     ccTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTg                                                                                                              <  1:644519/151‑1 (MQ=255)                                     ccTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTg                                                                                                              >  1:413312/1‑151 (MQ=255)                                         gCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGc                                                                                                          <  2:391804/151‑1 (MQ=255)                                           gATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCa                                                                                                         >  1:11639/1‑150 (MQ=255)                                                   tCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCt                                                                                                 <  1:286752/150‑1 (MQ=255)                                                    cAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCtt                                                                                                <  1:619499/150‑1 (MQ=255)                                                      gCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTa                                                                                             <  2:558922/151‑1 (MQ=255)                                                              ggCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTggg                                                                                      >  1:206394/1‑150 (MQ=255)                                                                    aaCATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAAc                                                                               >  1:575023/1‑151 (MQ=255)                                                                     aCATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAAc                                                                               >  1:474168/1‑150 (MQ=255)                                                                      cATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACtg                                                                             <  1:384224/151‑1 (MQ=255)                                                                         cACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACtgatg                                                                          <  1:601393/151‑1 (MQ=255)                                                                            cATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGatgatg                                                                       >  1:55853/1‑151 (MQ=255)                                                                                aaTACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGc                                                                   >  2:457601/1‑151 (MQ=255)                                                                                    ccGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTc                                                               <  1:537181/151‑1 (MQ=255)                                                                                          aCCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCt                                                         >  2:582450/1‑151 (MQ=255)                                                                                              gATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTgg                                                       <  2:526855/149‑1 (MQ=255)                                                                                               aTGTTGATGATTGCGGGTCTGCTGCTGCATAAAACGCCAATGGCTTATATGCGCGCTGTCAGCGCGGGTGCAGGCGGTACTGCCGGTATTCTGGTGCAATTCCCCTTCGGCGCTGGGATCCAACTGATGATGGAGCATTCCGGTcg                                                         <  2:462963/146‑2 (MQ=255)                                                                                                  tttATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGAc                                                 >  1:100828/1‑151 (MQ=255)                                                                                                     aTGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTc                                               >  2:87132/1‑150 (MQ=255)                                                                                                        cttGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGAATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGACGATGGAGCATTCCGGCCTGGGCGGACTCAtt                                            >  2:386208/2‑150 (MQ=255)                                                                                                            cGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCg                                        >  2:661658/1‑150 (MQ=255)                                                                                                              ggTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAAt                                     >  1:288091/1‑151 (MQ=255)                                                                                                               gTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAAtt                                    <  1:221842/151‑1 (MQ=255)                                                                                                                  tgctgcTACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAAttctt                                 >  1:253647/1‑151 (MQ=255)                                                                                                                    ctgctACATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAAttcttc                                >  2:635557/1‑150 (MQ=255)                                                                                                                          cATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATg                         >  1:295024/1‑151 (MQ=255)                                                                                                                          cATAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATg                         >  2:184573/1‑151 (MQ=255)                                                                                                                            tAAAACGCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGtt                       >  1:581263/1‑151 (MQ=255)                                                                                                                                 cGCCAAGGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGaa                  <  2:139355/151‑1 (MQ=255)                                                                                                                                  gCCAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAAc                 <  1:506173/151‑1 (MQ=255)                                                                                                                                   ccAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACa                <  1:414814/151‑1 (MQ=255)                                                                                                                                   ccAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACa                <  2:173571/151‑1 (MQ=255)                                                                                                                                    cAATGGCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACaa               <  2:12622/151‑1 (MQ=255)                                                                                                                                        ggCTTATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGa            <  2:462522/150‑1 (MQ=255)                                                                                                                                           ttATATGCGCGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGACAcc        >  1:227997/1‑151 (MQ=255)                                                                                                                                                 gcgcgcTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGACACCTTCCCg  <  2:600280/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TCTGACGTTGATCATCGGCGCACTCGGTATCGCTTACCTTGCGATGTACTTCAGCGAACATGGCTTCAACATCACCATCAATACCGTCAACCTGATGTTTATGATTGCGGGTCTGCTGCTACATAAAACGCCAATGGCTTATATGCGTGCTATCAGCGCGGCAGCACGCAGTACTGCCGGTATTCTGGTGCAATTCCCCTTCTACGCTGGGATCCAACTGATGATGGAGCATTCCGGTCTGGGCGGACTCATTACCGAATTCTTCATCAATGTTGCGAACAAAGACACCTTCCCG  >  NC_000913/2325490‑2325784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑