| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,367,405 | G→A | R112H (CGC→CAC) | arnC → | undecaprenyl phosphate‑L‑Ara4FN transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,367,405 | 0 | G | A | 85.5% | 144.0 / ‑4.4 | 55 | R112H (CGC→CAC) | arnC | undecaprenyl phosphate‑L‑Ara4FN transferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (21/26); minor base C (5/3); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGT > NC_000913/2367275‑2367550 | atTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGAt < 2:459335/150‑1 (MQ=255) aTTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTac < 1:415184/150‑1 (MQ=255) tttGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTacga < 1:171637/150‑1 (MQ=255) gCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTGACGTTACTGGGGGCTTAATTATTAAACTTTATGGCGCTTTCCAGAAACCGCCCGAAGAAAAACCCCCCCTGGTGGCGAAAGACGATGAAGGGTAAGAAGTg > 1:629018/1‑151 (MQ=255) tAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTACTTACGCTACTGGGGCCCTAATTTTTATCCTTTTTGGCCGTGTCCCGAAAACGCCCGAAAAAAAACCCCACCCTGTGGGAAAAGACGAGGAAGGGTACGAAGTgg > 2:625467/1‑149 (MQ=255) tAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGt < 2:272532/150‑1 (MQ=255) aaCCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAATCACAATTATGGCGGCTTAATTATTTACCTTTATGCCGATCTCCCGAATACCCCCGAAGAAAACCCCCACCCCGTGGCAAAAACCGATGAAGGGTACAACGTgg > 2:353537/1‑148 (MQ=255) cGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTACTCACGGTTTTGGGGGCTTTATTATTAACCTTTTTGCCGAGCTCCAGAATCCGCCCGAAAAAAAACCCCACCTCGTGGCAAAAACCGATGCAGGCTATGACGTGGGAggg > 2:471118/1‑150 (MQ=255) gCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGAc < 1:394605/151‑1 (MQ=255) cAATTACGGGCAACATTCAGCGATTATGGCGGGTTTCAGGCCCCTTATTTGCGGCTTTATTATTAACCTTTGTTACGGCCTCCAAAAACCGCCAGCAGAAAACCCCCCCCCGGGGGGAAAAAACGAAGGAGGGTACGACGTgggggg > 1:82930/1‑143 (MQ=255) aaTTACGGGCAACATTCAGCGATTATGGCGGGAGTTAGTCACCATTCTGGTGAACTAATTATTACTCTTGGTGTCGCATTCCCGAAGACCCCAGAAGAAAACCCCCCCCTCGTGGCAAAAACCGAAGAAAGTTAAAACGTGGTgggggc > 2:129773/1‑147 (MQ=255) gggCAACATTCAGCGATTATGGCGGTATTCCGTCACGTTGCTGGCGTCTTAATTATTACATTTGATGCCTATCTCCAGAGTCCGCATGAAGAAATCCCCCACCTAGTGGCAAAGTCCGGTGAAGGTTCCGGAATGGTAGGGTCTGTACGct < 2:34906/151‑2 (MQ=255) cATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAAcc < 2:502083/151‑1 (MQ=255) cATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAAcc < 2:434150/151‑1 (MQ=255) ttCAGCGATTATGGCGGGATTCAGTCACGCTACTTGCGACCTTATTATTTACCTTGATGCCGGTCTCCAGAAAACGCCAGAAGAAAAACCCCCCCCGGCGGGCAAAGACGGTGAAGGGTACGACGTGGGAGGGGCTGTACGCCCGaaac > 2:27714/1‑147 (MQ=255) cAGCGATTATGGCGGGATTCAGTCACGTTATTGGCGGCTTAATTATTATCCCTTATGCCGATCTCCAGAAACCCCCAGAAAAAAACCCCCCCCCGGGGGGAAAAACCGATGAAGGGTACGACGGGGGAGGGGCTGTACGCCCGAAACGc > 1:324591/1‑149 (MQ=255) cGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGa < 1:169027/151‑1 (MQ=255) gggATTCAGTCCCGTTACTGGCGACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAATTCCCCCCCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGt < 2:109316/149‑1 (MQ=255) ggATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTc < 1:123810/151‑1 (MQ=255) cAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTaa > 2:302271/1‑150 (MQ=255) cACGTTACTGGCGACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAAcc < 2:263990/150‑1 (MQ=255) cGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCGGGAC‑GCTGGGTTTGTAAAAACGcat > 1:116095/1‑149 (MQ=255) gTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCtt > 2:286949/1‑151 (MQ=255) tACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTc < 1:358129/150‑1 (MQ=255) aCTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCg < 1:484831/150‑1 (MQ=255) gACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAgatg > 2:543224/1‑150 (MQ=255) aCTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAgatgat > 2:491805/1‑151 (MQ=255) aCTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAgatgat > 2:659013/1‑151 (MQ=255) aCTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAgatgat < 1:373764/151‑1 (MQ=255) aCTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAgatga < 1:462440/150‑1 (MQ=255) tAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTGGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTa < 1:268734/150‑1 (MQ=255) tAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTa > 1:273910/1‑150 (MQ=255) aaTTATTCCCCTGGATGCCGATCTCCAGAAGCCGCCAGAAGAATCCCCCCCCCTGGTGGCAAAGGCCGATGAAGGTTACGACTTGGTAGGGACTTTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGAtt < 2:448399/148‑1 (MQ=255) aaTTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTaa < 2:187423/150‑1 (MQ=255) aaTTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAAc < 1:497016/151‑1 (MQ=255) tattaCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCgg > 2:247474/1‑151 (MQ=255) taCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCtt > 2:400861/1‑151 (MQ=255) cccTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTAt > 1:41561/1‑151 (MQ=255) cccTTGATGCCGACCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTAt < 2:231252/151‑1 (MQ=255) tGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTc > 1:325671/1‑149 (MQ=255) gATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCAAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAgc < 2:62544/151‑1 (MQ=255) aTGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAgcg < 1:245903/151‑1 (MQ=255) gATCTCCAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACACCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGcacc > 2:246317/1‑150 (MQ=255) ccAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGcaccac < 1:142679/147‑1 (MQ=255) ccAGAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTgg < 2:148179/150‑1 (MQ=255) gAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCaa > 2:355699/1‑150 (MQ=255) gAATCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCaa > 2:154576/1‑150 (MQ=255) aacccGCCAAAAGAAACCCCCCCCCGGGGGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACGGGCaag < 1:417188/147‑2 (MQ=255) tCCGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCg > 2:134724/1‑151 (MQ=255) ccGCCAGAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCg > 2:593879/1‑150 (MQ=255) ccGCCAAAAGAAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCg < 2:426862/150‑1 (MQ=255) agaagaAATCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGt > 2:217207/1‑151 (MQ=255) aaaTCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGTTGATTa < 2:354701/151‑1 (MQ=255) aaaTCCCCCACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGAtt > 2:24861/1‑150 (MQ=255) cccccACCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGt < 2:556989/151‑1 (MQ=255) | ATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGT > NC_000913/2367275‑2367550 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |