Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,435,409	C→T	A76A (GCG→GCA)	truA ←	tRNA pseudouridine(38‑40) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,435,409	0	C	T	100.0%	145.3 / NA	38	A76A (GCG→GCA)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/21);  total (17/21)

TGATTGTAGATGATGTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGT  >  NC_000913/2435263‑2435556                                                                                                                                                   |                                                                                                                                                    tgatTGTAGATGATGTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGCCATGAAAATCATCAGGTACAGTTTTAACACAACGCACAGAGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGACCACTCCGCGTCTTTGCGCAGTGCgg                                                                                                                                                 >  2:538738/1‑151 (MQ=255)     tGTAGCTGATGTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCCCCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGgttg                                                                                                                                              <  2:268049/150‑1 (MQ=255)            gatgTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGaa                                                                                                                                       <  1:620524/150‑1 (MQ=255)             atgTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAAt                                                                                                                                     >  2:14273/1‑151 (MQ=255)             atgTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAAt                                                                                                                                     <  1:501422/151‑1 (MQ=255)              tgTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATg                                                                                                                                    >  2:566700/1‑151 (MQ=255)               gTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGc                                                                                                                                   <  1:243525/151‑1 (MQ=255)                tAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGc                                                                                                                                   >  1:292074/1‑150 (MQ=255)                  gCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGcac                                                                                                                                 >  1:342068/1‑150 (MQ=255)                     aTAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAAcc                                                                                                                             <  1:467137/151‑1 (MQ=255)                       aaCGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTg                                                                                                                           <  1:536196/151‑1 (MQ=255)                                 cGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGACCGGTACCg                                                                                                                 >  2:222703/1‑151 (MQ=255)                                   tGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCgtg                                                                                                               >  1:361402/1‑151 (MQ=255)                                          aaaTCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTAcccc                                                                                                         >  2:648648/1‑150 (MQ=255)                                          aaaTCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCt                                                                                                        <  1:523490/151‑1 (MQ=255)                                           aaTCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTg                                                                                                       >  2:165568/1‑151 (MQ=255)                                              cGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGt                                                                                                    <  1:612101/151‑1 (MQ=255)                                              cGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGt                                                                                                    <  1:507209/151‑1 (MQ=255)                                                           catcaGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACACCTGCGTCAGTACGCCCgg                                                                                        <  1:351266/150‑1 (MQ=255)                                                              caGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCa                                                                                    <  1:124804/151‑1 (MQ=255)                                                                 gTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCaga                                                                                  >  2:242993/1‑150 (MQ=255)                                                                    cAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGAc                                                                              >  2:330534/1‑151 (MQ=255)                                                                          tAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGAt                                                                        >  1:519601/1‑151 (MQ=255)                                                                           aaCCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGTCGGTGa                                                                         <  2:370566/149‑1 (MQ=255)                                                                            aCCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATgg                                                                      >  2:659245/1‑151 (MQ=255)                                                                              ccAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGgt                                                                    <  1:575693/151‑1 (MQ=255)                                                                               cAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGgtt                                                                   <  2:314134/151‑1 (MQ=255)                                                                                 aCGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGgttcg                                                                 <  2:232643/151‑1 (MQ=255)                                                                                   gCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGgttcgtt                                                               <  2:153745/151‑1 (MQ=255)                                                                                         cGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCAc                                                         >  2:257784/1‑151 (MQ=255)                                                                                           aTGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCt                                                       <  1:350161/151‑1 (MQ=255)                                                                                                    ggTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAgcg                                              >  2:492492/1‑151 (MQ=255)                                                                                                        aaTTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCtt                                          >  2:48384/1‑151 (MQ=255)                                                                                                                      cccAAGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCt                            <  1:607178/151‑1 (MQ=255)                                                                                                                          aGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGcac                        <  1:45539/151‑1 (MQ=255)                                                                                                                          aGGTCCACGCCGCGTCTTTGCGCAGTGCGGTTGTTGCGAAATGCACAGCCTGCCCGGTACCGTGTACTCGTGCGTCAGTACGCCTGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGca                         <  1:352875/150‑1 (MQ=255)                                                                                                                                 cgccgcGTCTTTGCGCAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGCTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCgg                 >  2:408204/1‑151 (MQ=255)                                                                                                                                                cAGTGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGt  <  2:20368/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TGATTGTAGATGATGTAGCGATAACGGCGAGCCGTGGCGCTAAATCGGGCATGAAAATCATCAGGTACAGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGT  >  NC_000913/2435263‑2435556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑