Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,441,945	T→C	V61A (GTG→GCG)	mnmC →	fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,441,945	0	T	C	100.0%	189.9 / NA	52	V61A (GTG→GCG)	mnmC	fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/25);  total (27/25)

TTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTT  >  NC_000913/2441801‑2442092                                                                                                                                                 |                                                                                                                                                    ttAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAg                                                                                                                                                <  1:400018/150‑1 (MQ=255)  tAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGc                                                                                                                                               <  1:170188/150‑1 (MQ=255)         gAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGCTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCg                                                                                                                                       <  2:452582/151‑1 (MQ=255)         gAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCAATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCg                                                                                                                                       <  1:492816/151‑1 (MQ=255)                cacCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCgg                                                                                                                                >  2:291942/1‑151 (MQ=255)                 acCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGc                                                                                                                               >  2:542824/1‑151 (MQ=255)                 acCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGc                                                                                                                               <  1:190008/151‑1 (MQ=255)                      tttCCCGAGATTTTGACGATGTCTATTTTTCCAACCATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCgg                                                                                                                          >  1:59639/1‑151 (MQ=255)                       ttCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCgg                                                                                                                          >  1:365969/1‑150 (MQ=255)                           cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTaa                                                                                                                     <  1:423658/151‑1 (MQ=255)                           cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTa                                                                                                                      >  2:465553/1‑150 (MQ=255)                           cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTa                                                                                                                      >  2:26013/1‑150 (MQ=255)                            gagaTTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTaa                                                                                                                     >  2:90842/1‑150 (MQ=255)                               aTTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACtt                                                                                                                 <  2:218220/151‑1 (MQ=255)                                             tATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATggc                                                                                                   >  2:168116/1‑151 (MQ=255)                                              aTTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATggca                                                                                                  <  1:419026/151‑1 (MQ=255)                                              aTTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATggc                                                                                                   <  1:16841/150‑1 (MQ=255)                                                ttttCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATggcagg                                                                                                >  2:95856/1‑151 (MQ=255)                                                    cgaaCGATAACGGGCTGGCAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCAtt                                                                                            >  2:360707/3‑151 (MQ=255)                                                               gggCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTc                                                                                 >  2:92319/1‑151 (MQ=255)                                                                ggCTGGAAGAGACGCGTTATGTTTTTCTGGGACGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAGCTTCCTGACGCTATGGCAGGCATTTGATCAGTTTcg                                                                                <  2:61308/151‑1 (MQ=255)                                                                        gagaCGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAgcgc                                                                        <  1:180792/151‑1 (MQ=255)                                                                           aCGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATc                                                                     >  2:285425/1‑151 (MQ=255)                                                                                  aTGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAgc                                                              <  1:544625/151‑1 (MQ=255)                                                                                  aTGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAgc                                                              >  2:158890/1‑151 (MQ=255)                                                                                    gTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAgcgc                                                            >  1:591393/1‑151 (MQ=255)                                                                                     tttttCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAgcgc                                                            >  2:388256/1‑150 (MQ=255)                                                                                            gggAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACaa                                                    <  2:672089/151‑1 (MQ=255)                                                                                            gggAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACa                                                     <  1:26492/150‑1 (MQ=255)                                                                                               aGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGc                                                 <  1:381424/151‑1 (MQ=255)                                                                                               aGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGc                                                 <  1:296937/151‑1 (MQ=255)                                                                                                ggCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCt                                                <  2:371818/151‑1 (MQ=255)                                                                                                 gCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCtt                                               >  2:458813/1‑151 (MQ=255)                                                                                                  cAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCtt                                               >  2:398221/1‑150 (MQ=255)                                                                                                  cAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTa                                              <  1:348437/151‑1 (MQ=255)                                                                                                   aaCCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTAc                                             <  2:386374/151‑1 (MQ=255)                                                                                                       aaTTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACAttt                                         >  1:471227/1‑151 (MQ=255)                                                                                                        cttAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACAttt                                         >  2:162289/2‑150 (MQ=255)                                                                                                         ttAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTc                                        >  1:348131/1‑150 (MQ=255)                                                                                                           agagGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCAtt                                     >  1:637511/1‑151 (MQ=255)                                                                                                             agGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCAtt                                     >  2:180337/1‑149 (MQ=255)                                                                                                             agGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAg                                   <  1:639679/151‑1 (MQ=255)                                                                                                              ggTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGt                                  >  1:359449/1‑151 (MQ=255)                                                                                                               gTACGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGtt                                 <  2:163878/151‑1 (MQ=255)                                                                                                                 aCGCTTTCCTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGtttt                               <  1:193939/151‑1 (MQ=255)                                                                                                                        ccTGAGCATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGaaa                         <  2:402471/150‑1 (MQ=255)                                                                                                                              cATCCACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTcccc                  >  1:285218/1‑151 (MQ=255)                                                                                                                                 ccACATCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCa               <  1:435062/151‑1 (MQ=255)                                                                                                                                  cacaTCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCAc              >  2:372550/1‑151 (MQ=255)                                                                                                                                     aTCCTCTGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCg           <  2:618118/151‑1 (MQ=255)                                                                                                                                           tGTTTGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCgg      >  2:218952/1‑150 (MQ=255)                                                                                                                                              ttGCGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGAttt  >  2:588516/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                    TTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTTGATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCGTGCGGATTT  >  NC_000913/2441801‑2442092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑