Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,471,762	G→C	pseudogene (361/579 nt)	yfdL ←	pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,471,762	0	G	C	100.0%	147.5 / NA	43	pseudogene (361/579 nt)	yfdL	pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (17/26);  total (17/26)

CCTGGTTATAACCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGA  >  NC_000913/2471615‑2471907                                                                                                                                                    |                                                                                                                                                  ccTGGTTATAACCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGcccc                                                                                                                                                >  1:181917/1‑151 (MQ=255)        ataACCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTg                                                                                                                                          <  1:515040/150‑1 (MQ=255)             cGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGaataat                                                                                                                                    <  2:574925/151‑1 (MQ=255)                       tatataGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGAt                                                                                                                          >  1:118240/1‑151 (MQ=255)                       tatataGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGAt                                                                                                                          >  1:245046/1‑151 (MQ=255)                        atataGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATg                                                                                                                         <  2:249116/151‑1 (MQ=255)                              ccAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAAtt                                                                                                                   <  2:451881/151‑1 (MQ=255)                                aaCATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAAtttt                                                                                                                 <  2:20816/151‑1 (MQ=255)                                 aCATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAAttttt                                                                                                                <  2:672555/151‑1 (MQ=255)                                  cATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTg                                                                                                               <  2:547754/151‑1 (MQ=255)                                       gCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATc                                                                                                          <  1:587848/151‑1 (MQ=255)                                         aCTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATcc                                                                                                         >  1:294066/1‑150 (MQ=255)                                            gCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAgg                                                                                                      >  2:353932/1‑150 (MQ=255)                                             ccGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGa                                                                                                     >  2:2593/1‑150 (MQ=255)                                                 gAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGa                                                                                                <  2:566685/151‑1 (MQ=255)                                                        aTCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGaa                                                                                         <  2:486116/151‑1 (MQ=255)                                                            tGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGc                                                                                      >  2:144953/1‑150 (MQ=255)                                                             gAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGc                                                                                      <  1:229827/149‑1 (MQ=255)                                                                  gCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAAc                                                                               <  1:314178/151‑1 (MQ=255)                                                                       tACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTAccc                                                                          >  1:238390/1‑151 (MQ=255)                                                                        aCCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTAccc                                                                          >  2:412815/1‑150 (MQ=255)                                                                           cGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCatat                                                                      <  1:300420/151‑1 (MQ=255)                                                                            gCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATc                                                                     <  2:154483/151‑1 (MQ=255)                                                                               taCATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAgg                                                                  >  2:569993/2‑151 (MQ=255)                                                                                aCATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGt                                                                 >  2:266542/1‑151 (MQ=255)                                                                                      aTTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTg                                                            <  2:342011/150‑1 (MQ=255)                                                                                           gAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGc                                                      <  1:213477/151‑1 (MQ=255)                                                                                            caaTGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCACATCAGGTATCTGATTCGcc                                                     >  2:122998/2‑151 (MQ=255)                                                                                             aaTGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGccc                                                    >  1:138976/1‑151 (MQ=255)                                                                                               tGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCt                                                  >  2:367028/1‑151 (MQ=255)                                                                                                gCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCt                                                  >  1:46172/1‑150 (MQ=255)                                                                                                 cAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGt                                                >  2:518727/1‑151 (MQ=255)                                                                                                     cGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTccc                                             <  1:172806/150‑1 (MQ=255)                                                                                                        aaaaTAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACa                                         <  1:287099/151‑1 (MQ=255)                                                                                                          aaTAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACAtt                                       <  1:583154/151‑1 (MQ=255)                                                                                                             aGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCt                                    <  2:408917/151‑1 (MQ=255)                                                                                                               gTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCttt                                  <  1:88445/151‑1 (MQ=255)                                                                                                                 tACTGTGTCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCtttt                                 <  2:117059/150‑1 (MQ=255)                                                                                                                      tgtCCTGAGACGTCAGCGGAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGc                           >  2:524637/1‑151 (MQ=255)                                                                                                                                        gAACATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTGTAGCCGCTTCTCCCAAACCAAGGtt         <  2:458648/151‑1 (MQ=255)                                                                                                                                         aaCATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGttt        <  1:354989/151‑1 (MQ=255)                                                                                                                                           cATCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCGAACCAAGGTTTTc      <  2:262669/151‑1 (MQ=255)                                                                                                                                             tCAATTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCg     >  1:325210/1‑150 (MQ=255)                                                                                                                                               aaTTGCCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgaga  <  2:592732/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CCTGGTTATAACCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGA  >  NC_000913/2471615‑2471907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑