Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,477,942	G→A	A32A (GCG→GCA)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,477,942	0	G	A	100.0%	130.0 / NA	34	A32A (GCG→GCA)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/15);  total (19/15)

TGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATG  >  NC_000913/2477794‑2478083                                                                                                                                                     |                                                                                                                                              tGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACt                                                                                                                                             >  2:104056/1‑151 (MQ=255)       gttTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCt                                                                                                                                       <  2:5537/151‑1 (MQ=255)                  cAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGcttct                                                                                                                            >  1:231411/1‑151 (MQ=255)                       tcatcaGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCg                                                                                                                         >  1:357002/1‑149 (MQ=255)                         atcaGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTggg                                                                                                                     <  2:336609/151‑1 (MQ=255)                           caGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGa                                                                                                                    <  2:354013/150‑1 (MQ=255)                               ttAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACgat                                                                                                               >  1:377659/1‑151 (MQ=255)                                tAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACgatg                                                                                                              >  1:201925/1‑151 (MQ=255)                                  aTCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACgatg                                                                                                              >  2:211109/1‑149 (MQ=255)                                    cacaGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATgg                                                                                                          >  1:428333/1‑151 (MQ=255)                                             aGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGc                                                                                                 >  1:27935/1‑151 (MQ=255)                                                 gagaTATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACt                                                                                             <  2:15161/151‑1 (MQ=255)                                                 gagaTATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACt                                                                                             >  2:214598/1‑151 (MQ=255)                                                   gaTATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTgg                                                                                           >  2:244865/1‑151 (MQ=255)                                                     tatGCACTCTCAAATCTGGGTTGTGAGCCCGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGa                                                                                          <  1:244870/150‑1 (MQ=255)                                                        gCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATg                                                                                      <  1:350190/151‑1 (MQ=255)                                                               cAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTaaa                                                                                 <  1:516328/149‑1 (MQ=255)                                                                      gggTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTg                                                                         >  1:98783/1‑150 (MQ=255)                                                                           gtgAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTg                                                                   <  2:202209/151‑1 (MQ=255)                                                                              aGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGa                                                                 >  1:478155/1‑150 (MQ=255)                                                                                cACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAAtt                                                              >  1:37128/1‑151 (MQ=255)                                                                                 aCGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTg                                                             <  2:318417/151‑1 (MQ=255)                                                                                   gctgctTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGc                                                           >  2:557850/1‑151 (MQ=255)                                                                                    ctgctTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCg                                                          >  2:190159/1‑151 (MQ=255)                                                                                                                  aCCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCg                                   >  1:541123/1‑144 (MQ=255)                                                                                                                  aCCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCg                                   <  2:541108/144‑1 (MQ=255)                                                                                                                           aaGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGc                    >  1:542864/1‑150 (MQ=255)                                                                                                                            aGTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCAc                  <  2:264758/151‑1 (MQ=255)                                                                                                                             gTTCAAATTCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACg                 >  1:415058/1‑151 (MQ=255)                                                                                                                                     tCCACCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTgg          <  1:135857/150‑1 (MQ=255)                                                                                                                                        aCCCGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGaaa      <  1:184838/151‑1 (MQ=255)                                                                                                                                           cGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAat   <  1:437955/151‑1 (MQ=255)                                                                                                                                           cGTTTCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAat   <  1:424922/151‑1 (MQ=255)                                                                                                                                             tttCTGGCACTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAatg  >  2:324983/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                              TGCGTTGTTTTTTTGTCCAATATCATCAGGTTAATCACAGGGGAAGGTGAGATATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTGACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATG  >  NC_000913/2477794‑2478083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑