Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,536,495	G→A	A248V (GCG→GTG)	pdxK ←	pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,536,495	0	G	A	100.0%	138.1 / NA	36	A248V (GCG→GTG)	pdxK	pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/15);  total (21/15)

GAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCT  >  NC_000913/2536357‑2536635                                                                                                                                           |                                                                                                                                             gAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCa                                                                                                                                  <  1:632892/151‑1 (MQ=255)  aaaaaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTc                                                                                                                                   >  1:214416/1‑149 (MQ=255)     aaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCa                                                                                                                               >  2:325245/1‑150 (MQ=255)       tGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGc                                                                                                                            >  2:86179/1‑151 (MQ=255)            cccATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtg                                                                                                                       >  2:513365/1‑151 (MQ=255)                 cGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtgatga                                                                                                                   <  2:574482/150‑1 (MQ=255)                 cGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtgatg                                                                                                                    >  2:61676/1‑149 (MQ=255)                      ccATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagctgag                                                                                                             <  2:470645/151‑1 (MQ=255)                        aTTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagctgagc                                                                                                            >  1:613039/1‑150 (MQ=255)                           tttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGcaca                                                                                                         >  2:272956/1‑150 (MQ=255)                           tttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGcaca                                                                                                         >  2:263778/1‑150 (MQ=255)                              ttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACaaaa                                                                                                      <  1:74284/150‑1 (MQ=255)                                  gCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAgg                                                                                                  <  1:514831/150‑1 (MQ=255)                                     tCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTc                                                                                                <  1:364208/149‑1 (MQ=255)                                      ccGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGcc                                                                                             >  1:205380/1‑151 (MQ=255)                                       cGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCa                                                                                            <  1:251068/151‑1 (MQ=255)                                         ccAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGt                                                                                          >  2:278565/1‑151 (MQ=255)                                                 ggcAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTc                                                                                   >  1:6872/1‑150 (MQ=255)                                                    aaaaTCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGt                                                                               <  2:135634/151‑1 (MQ=255)                                                           aTTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGtttt                                                                        <  2:18282/151‑1 (MQ=255)                                                              cATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTAc                                                                     >  2:359794/1‑151 (MQ=255)                                                                 cGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCg                                                                  <  1:492971/151‑1 (MQ=255)                                                                  gCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGt                                                                 <  2:108476/151‑1 (MQ=255)                                                                       cATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATg                                                            <  1:651368/151‑1 (MQ=255)                                                                         tgctgcTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATggg                                                          >  2:59859/1‑151 (MQ=255)                                                                                gggTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATg                                                     <  1:488703/149‑1 (MQ=255)                                                                                gggTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGa                                                    >  1:347740/1‑150 (MQ=255)                                                                                           aTCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGt                                        >  1:2860/1‑151 (MQ=255)                                                                                              aCTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCgg                                     >  1:6115/1‑151 (MQ=255)                                                                                                 tCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGc                                    >  1:314809/1‑149 (MQ=255)                                                                                                  ccAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGt                                 <  1:644054/151‑1 (MQ=255)                                                                                                        cgcgCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGaccac                           >  1:125042/1‑151 (MQ=255)                                                                                                              aCCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAAc                     <  1:626248/151‑1 (MQ=255)                                                                                                                       ggTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCAtctc            >  1:627511/1‑151 (MQ=255)                                                                                                                             cTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGAtt      >  2:589194/1‑151 (MQ=255)                                                                                                                                 aTCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTttct  >  1:637175/1‑151 (MQ=255)                                                                                                                                           |                                                                                                                                             GAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCT  >  NC_000913/2536357‑2536635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑